6 gingerol

Manufactured by Merck Group

Sourced in United States, Germany, India, United Kingdom

6-gingerol is a chemical compound found in ginger root. It is used as a reference standard in laboratory analysis and research applications. The core function of 6-gingerol is to serve as a marker compound for the identification and quantification of ginger in various samples.

Automatically generated - may contain errors

Lab products found in correlation

6 shogaol, by Merck Group (9 mentions) 8 gingerol, by Merck Group (4 mentions) Dimethyl sulfoxide (dmso), by Merck Group (4 mentions) Formic acid, by Merck Group (4 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions) 10 gingerol, by Merck Group (3 mentions) Zingerone, by Merck Group (3 mentions) Eugenol, by Merck Group (2 mentions) Bovine serum albumin (bsa), by Merck Group (2 mentions) Methanol, by Merck Group (2 mentions) 6 paradol, by Merck Group (2 mentions) Naringin, by Merck Group (2 mentions) 10 shogaol, by Merck Group (2 mentions) Dimethyl sulfoxide (dmso), by Avantor (2 mentions) Tnf α, by Abcam (2 mentions) Dimethyl sulfoxide (dmso), by Carl Roth (2 mentions) Trypsin edta, by Thermo Fisher Scientific (2 mentions) Pe conjugated anti ifn γ, by BD (1 mentions) Fitc conjugated anti cd4, by BD (1 mentions) Fluorescein isothiocyanate fitc conjugated anti cd11b, by BD (1 mentions)

42 protocols using 6 gingerol

Osteoblast-like MG63 Cell Culturing and 6-Gingerol Treatment

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human osteoblast-like MG63 cells (ATCC CRL-1427) were obtained from American Type
Culture Collection (USA) and maintained in Dulbecco's modified Eagle's medium (DMEM;
Gibco BRL, USA) supplemented with 10% fetal bovine serum (FBS; Gibco BRL) and 100
µg/mL penicillin-streptomycin at 37°C in a humidified atmosphere containing 5%
CO₂. Cells were seeded on six-well culture plates at an initial density
of 1×10⁵ cells/mL and grown to approximately 80% confluence. For treatment
with 6-gingerol, MG-63 cells were pretreated with 1% FBS DMEM for 16 h, and then
treated with TNF-α (10 ng/mL) followed by exposure to 6-gingerol (Sigma, USA) with
serial concentrations (0-50 µM) in serum-free DMEM for 24 or 48 h. After the
6-gingerol treatment, the cells were washed with phosphate-buffered saline (PBS; 25
mM sodium phosphate, 150 mM NaCl, pH 7.2) and collected for the following
analyses.

Fan J.Z., Yang X, & Bi Z.G. (2015). The effects of 6-gingerol on proliferation, differentiation, and maturation of osteoblast-like MG-63 cells. Brazilian Journal of Medical and Biological Research, 48(7), 637-643.

+ Open protocol

+ Expand

Ginger Compound Modulates Immune Responses

Check if the same lab product or an alternative is used in the 5 most similar protocols

6-Gingerol (Sigma-Aldrich, St Louis, MO) was dissolved to 3.5 μg/ml of 50% ethanol and diluted with drinking water, as required (the final ethanol concentration was less than 0.01%). The following antibodies were purchased from BD Pharmingen, San Diego, CA: phycoerythrin- (PE-) conjugated anti-Ly6G, fluorescein isothiocyanate- (FITC-) conjugated anti-CD11b, PE-conjugated anti-CXCR2, FITC-conjugated anti-CD4, FITC-conjugated anti-CD8, PE-conjugated anti-IFN-γ, PE-conjugated anti-CD4, PE-conjugated anti-CD8, and FITC-conjugated antiannexin V antibodies. P. aeruginosa (ATCC27853) was obtained from the American Type Culture Collection (ATCC). For the M1 and M2 macrophage analysis, cells were separately stained with monoclonal antibodies specific for F4/80-PE from eBioscience; F4/80-FITC and CD206-PerCP/Cy5.5 from BioLegend, San Diego, CA; and CD80-FITC and iNOS-FITC from BD Transduction Laboratories, San Diego, CA. Bacteria were grown in brain heart infusion (BHI) media (Difco) at 37°C for 18 h, and aliquots were frozen at −80°C.

Ju S.A., Nguyen Q.T., Nguyen T.H., Suh J.H., An W.G., Callaway Z., Joe Y., Chung H.T, & Kim B.S. (2021). Pretreatment with 6-Gingerol Ameliorates Sepsis-Induced Immune Dysfunction by Regulating the Cytokine Balance and Reducing Lymphocyte Apoptosis. Oxidative Medicine and Cellular Longevity, 2021, 5427153.

+ Open protocol

+ Expand

Streptozotocin-Induced Diabetic Model: Antioxidant and Anti-inflammatory Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The diabetes inducing drug, Streptozotocin was purchased from Abcam (ab 142155, Cambridge, UK), 6-gingerol (G1045) was purchased Sigma Aldrich, St. Louis, MO, USA. Antioxidant enzymes like superoxide dismutase (SOD) (ab 65354), Glutathione-S-transferase (GST) (ab 65326), catalase (CAT) (ab 83464) and Trichrome Stain Kit (Connective Tissue Stain) (ab150686) were bought from Abcam, Cambridge, UK. ELISA kits for the assay of inflammation by C-reactive protein (ab 108827), TNF-α (ab 46070), IL-1β (ab 100768), and IL-6 (ab 100772) were procured from Abcam, Cambridge, UK. Primary antibodies (TNF-α) and Goat Anti-Mouse IgG H&L (HRP) (abcam, Cambridge, UK) used in this study were purchased from Abcam, Cambridge, UK. All supplementary chemicals used in this study were of high purity grade obtained from commercial sources.

Almatroodi S.A., Alnuqaydan A.M., Babiker A.Y., Almogbel M.A., Khan A.A, & Husain Rahmani A. (2021). 6-Gingerol, a Bioactive Compound of Ginger Attenuates Renal Damage in Streptozotocin-Induced Diabetic Rats by Regulating the Oxidative Stress and Inflammation. Pharmaceutics, 13(3), 317.

+ Open protocol

+ Expand

Fingerprinting of Endocannabinoid System

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fingerprinting eCS was performed as described elsewhere [20 (link)], with the mobile phase composed of 0.1% formic acid (A) and acetonitrile (B) in water. The conditions of solvent gradient elution were 20% B in 0–3 min, 20% B in 5 min, 30% B in 12 min, 35% B in 16 min, 60% B in 20 min, 80% B in 30 min, 80% B in 34 min, 60% B in 37 min, 20% B in 40 min. Fifteen μL of eCS was run at the flow rate of 0.5 mL/min and 37 °C. All the chemicals were detected at wavelengths of 254 ~ 360 nm. The retention time of each chemical was compared to those of standard chemicals for identification. Chemical standards, such as chlorogenic acid, rosmarinic acid, Eugenol, 6-Gingerol, and aristolochic acid I, were obtained from Sigma-Aldrich (Seoul, Korea).

Han J.W., Kim K.H., Kwun M.J., Choi J.Y., Kim S.J., Jeong S.I., Lee B.J., Kim K.I., Won R., Jung J.H., Jung H.J, & Joo M. (2019). Suppression of lung inflammation by the ethanol extract of Chung-Sang and the possible role of Nrf2. BMC Complementary and Alternative Medicine, 19, 15.

+ Open protocol

+ Expand

DPPH-Based Antioxidant Activity Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antioxidant activity of the extracts were estimated using DPPH in vitro method Mensor et al. [18] (link). Test samples were dissolved separately in methanol to get test solution of 1 mg/mL. Series of extract/ pure components (6-gingerol and 6-shogaol; purchased from (Sigma-Aldrich GmbH, Germany) solutions of different concentrations (100, 200, 400, 600, 800 and 1000 µg/mL) were prepared by diluting with methanol. Assays were performed in 96-well, microtiter plates. 140 µL of 0.6 × 10⁻⁶ mol/L DPPH were added to each well containing 70 µL of sample. The mixture was shaken gently and left to stand for 30 min in dark at room temperature. The absorbance was measured spectrophotometrically at 517 nm using Cecil-Elect Spectrophotometer. Blank was done in the same way using methanol and sample without DPPH and control was done in the same way but using DPPH and methanol without sample. Ascorbic acid was used as reference antioxidant compound. Every analyse is done in triplicate. The ability to scavenge DPPH radical was calculated by the following equation:

DPPH radical scavenging activity (%) = 100 - [({Abs}_{sample} - {Abs}_{blank}) \times 100] / ({Abs}_{control})]

where

Abs_sample is the absorbance of DPPH radical + sample;

Abs_blank is the absorbance of sample + methanol;

Abs_control is the absorbance of DPPH radical + methanol.

Ali A.M., El-Nour M.E, & Yagi S.M. (2018). Total phenolic and flavonoid contents and antioxidant activity of ginger (Zingiber officinale Rosc.) rhizome, callus and callus treated with some elicitors. Journal of Genetic Engineering & Biotechnology, 16(2), 677-682.

+ Open protocol

+ Expand

Vascular Reactivity Assessment Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

6-Gingerol, Nω-nitro-l-arginine methyl ester hydrochloride (l-NAME), methylene blue (MB), tetraethylammonium chloride (TEA), indomethacin (INDO), aminoguanidine (AG), ribose, bovine serum albumin (BSA), acetylcholine (ACh), and phenylephrine (PE) were purchased from Sigma-Aldrich Co., St Louis, MO, USA, and 4-amino-5-methylamino-2′,7′-difluorofluorescein (DAF-FM) diacetate was purchased from Molecular Probes, NY, USA. All chemicals were dissolved in ultrapure deionized water except for 6-Gingerol, DAF-FM diacetate, and INDO, which were dissolved in dimethyl sulfoxide (DMSO). The final DMSO concentration did not exceed 0.1% that has no effect on vascular reactivity according to our preliminary studies.

Ghareib S.A., El-Bassossy H.M., Elberry A.A., Azhar A., Watson M.L, & Banjar Z.M. (2015). 6-Gingerol alleviates exaggerated vasoconstriction in diabetic rat aorta through direct vasodilation and nitric oxide generation. Drug Design, Development and Therapy, 9, 6019-6026.

+ Open protocol

+ Expand

Apoptosis Induction in Meningioma Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human meningioma cell lines (IOMM-Lee, CH157MN) were obtained from Elmar Kirchesa (Institute of Neuropathology, Otto-von-Guericke-University, Germany) and Jerry Jeff Jaboin (Washington University of Medicine, USA) [22 (link)–25 (link)]. Human neuron culture was purchased from ScienceCell Research Laboratories and maintained under the conditions provided by ScienceCell Research Laboratories. Standard laboratory techniques were used to grow and treat these cells. Drug stock solutions were prepared in dimethyl sulfoxide (DMSO). An equal amount of DMSO (0.01 %) was added to untreated control cells. Dose-response studies were conducted to determine the suitable concentration of the drugs used in the experiments [26 (link), 27 (link)]. Cells were treated with 25 μM Limonin (LKT Laboratories), 25 μM Tangeritin (LKT Laboratories), 25 μM 6-Gingerol (Sigma-Aldrich), 25 μM Zerumbone (LKT Laboratories), 25 μM Ganoderic Acid A (ChromaDex), 25 μM Ganoderic Acid DM (ChemFaces), and 25 μM hydroxyurea (Sigma) for 24 h for induction of apoptosis. After treatments, cells were used for determination of the mechanisms of apoptosis.

Das A., Miller R., Lee P., Holden C.A., Lindhorst S.M., Jaboin J., Vandergrift WA I.I.I., Banik N.L., Giglio P., Varma A.K., Raizer J.J, & Patel S.J. (2015). A novel component from citrus, ginger, and mushroom family exhibits antitumor activity on human meningioma cells through suppressing the Wnt/β-catenin signaling pathway. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 36(9), 7027-7034.

+ Open protocol

+ Expand

Preparation of 6-Gingerol Stock Solution

Check if the same lab product or an alternative is used in the 5 most similar protocols

6-gingerol was obtained from Sigma-Aldrich (St. Louis, MO, USA. catalog # G1046). It was dissolved in DMSO in a stock of 100 mmol/L and was diluted in cell culture media for experimental uses.

Lin S, & Lu P. (2024). Ginger Root Bioactive Compounds Specifically Inhibits Growth of Colon Cancer Cells in Culture. Nutrition and Metabolic Insights, 17, 11786388241231163.

+ Open protocol

+ Expand

Fluorescent Lipophilic Dyes and Immune Cell Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

The fluorescent lipophilic dyes, 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiL), 3,3′-dioctadecyloxacarbocyanine perchlorate (DiO) and 1,1′-dioctadecyl-3,3′,3′-tetramethylindotricarbocyanine iodide (DiR), were purchased from Promokine (Heidelberg, Germany); DC-Chol/DOPE Blend was from Avanti Polar Lipids (Alabaster, AL, USA); phalloidin-FITC, O-dianisidine dihydrochloride, myeloperoxidase from human leukocytes, type VIII collagenase, DNase I, (6)-gingerol and (6)-shogaol standards were purchased from Sigma (St. Louis, MO, USA). Rabbit anti-mouse E-cadherin antibody was from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-mouse CD326 (EpCAM) PE-Cy7, anti-mouse CD11b eFluo 450; anti-mouse CD11c APC, and anti-mouse F4/80 antigen PE-Cy7 were purchased from eBioscience (San Diego, CA, USA). Duoset enzyme-linked immunosorbent assay (ELISA) kits were purchased from R&D Systems (Minneapolis, MN, USA).

Zhang M., Viennois E., Prasad M., Zhang Y., Wang L., Zhang Z., Han M.K., Xiao B., Xu C., Srinivasan S, & Merlin D. (2016). Edible Ginger-Derived Nanoparticles: A Novel Therapeutic Approach for the Prevention and Treatment of Inflammatory Bowel Disease and Colitis-Associated Cancer. Biomaterials, 101, 321-340.

+ Open protocol

+ Expand

Comprehensive Phytochemical Protocol Inventory

Check if the same lab product or an alternative is used in the 5 most similar protocols

Indigo, Aliso B acetate, gentiopicroside, peimine, perillaldehyde, wedelolactone, bergapten, picroside I, berberine, cinnamaldehyde, ephedrine, p-synephrine, 6-shogao, and 6-gingerol were purchased from Sigma-Aldrich. Phellodendrine was purchased from Abcam. Baicalein, baicalin, wogonin, wogonoside, and Ephedra sinica were kindly provided by Y.L. Leu (Chang Gung University, Taiwan). Scutellaria baicalensis (precursor of Baicalein, baicalin, wogonin, wogonoside) and Ephedra sinica were supplied and authenticated by Department of Pharmacy Services, Chang Gung Memorial Hospital at Taoyuan, Taiwan. See Supplementary Information for more details on compound preparation.

Lin Y.H., Luck H., Khan S., Schneeberger P.H., Tsai S., Clemente-Casares X., Lei H., Leu Y.L., Chan Y.T., Chen H.Y., Yang S.H., Coburn B., Winer S, & Winer D.A. (2019). Aryl hydrocarbon receptor agonist indigo protects against obesity-related insulin resistance through modulation of intestinal and metabolic tissue immunity. International Journal of Obesity (2005), 43(12), 2407-2421.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!