Ng and Pt were grown in f/2 14x or RAS wastewater added with 14-fold concentrated NaNO3 (14N) using a Multicultivator MC 1000 OD (Photon System Instruments, Drásov, Check Republic) in flasks containing 80 mL of liquid culture at 22°C with a constant light intensity of 100 µmol photons m−2 s−1 and with air bubbling. The cultures were grown in triplicates until the stationary phase was reached (i.e., 18 days).
Multi cultivator mc 1000 od
Manufactured by Photon Systems Instruments
Sourced in Czechia
The Multi-Cultivator MC 1000-OD is a laboratory equipment designed for the cultivation of microorganisms. It provides a controlled environment for the growth of various types of cells or organisms. The device features independent temperature and lighting control for each cultivation chamber.
Automatically generated - may contain errors
Lab products found in correlation
9 protocols using multi cultivator mc 1000 od
1
Growth of Ng and Pt in Wastewater
Check if the same lab product or an alternative is used in the 5 most similar protocols
Ng and Pt were grown in f/2 14x or RAS wastewater added with 14-fold concentrated NaNO3 (14N) using a Multicultivator MC 1000 OD (Photon System Instruments, Drásov, Check Republic) in flasks containing 80 mL of liquid culture at 22°C with a constant light intensity of 100 µmol photons m−2 s−1 and with air bubbling. The cultures were grown in triplicates until the stationary phase was reached (i.e., 18 days).
+ Expand
Ng and Pt were grown in f/2 14x or RAS wastewater added with 14-fold concentrated NaNO3 (14N) using a Multicultivator MC 1000 OD (Photon System Instruments, Drásov, Check Republic) in flasks containing 80 mL of liquid culture at 22°C with a constant light intensity of 100 µmol photons m−2 s−1 and with air bubbling. The cultures were grown in triplicates until the stationary phase was reached (i.e., 18 days).
2
Characterizing C. reinhardtii Growth under Acetate
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Strains WT and iclC were described previously ([2 (link),18 (link)] and are both derived from the 137 C strain of C. reinhardtii [19 (link)]. They were first adapted for 48 h in Tris-Phosphate medium [4 ], adjusted to pH 7.0 with HCl, with different acetate concentrations (17, 31, 44, 57, and 87 mM sodium acetate) in flasks or in small photobioreactors (Multi-Cultivator MC 1000-OD, Photon Systems Instruments). Experimental cultivations were started with an initial cell concentration around 2 × 105 cells per mL, at 26 ± 1 °C, under moderate light (50 µmol.m−2.s−1), and followed for 145 h. Cells were counted using a Coulter counter (http://www.beckmancoulter.com ). The number of divisions per day was calculated according to [20 (link)]. Cell size (maximal diameter, estimated on 100 cells) was determined under a microscope, using the Imaging Software, NIS Elements, version 4, for Nikon (Nikon Instruments Inc., Melville, NY, USA).
3
Algae Stress Response Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cultures were transferred from standard growth conditions at 12 °C and 100 µmol photons m−2 s−1 to 2, 12, 22 and 32 (±1) °C for one week at 100 and 500 µmol photons m−2 s−1 (n = 8) in a light:dark cycle of 16:8 in a Multi-Cultivator MC 1000-OD (Photon Systems Instruments, Brno, Czech Republic). Additionally, algae were cultured at 2 °C in darkness for one week. Sampling was performed initially (1 h), short-term (16 h) and long-term (1 week), replicates n = 8. An external cooling aggregate was mounted to the MC for the treatments at 2 and 12 °C. Exposures to UV-radiation (UVR) were performed short-term (16 h) at all mentioned temperatures for high and low light (500 and 100 µmol photons m−2 s−1) exposure in a sun simulator (SonSi, iSiTECGmbH, Bremerhaven, Germany). For a solar-like spectrum including UVR (UVA 8.8 Wm−2, UVB 0.61 W m−2), the cultures were illuminated with a 400 W Metallogen lamp (Philips MSR 400 HR, Germany), as described in literature29 (link),30 (link). After exposure, samples from all treatments were collected. Additionally, samples from standard culture conditions and from one day, one week and one month old cultures (n = 3) were taken. Samples were centrifuged in 2 mL reaction tubes (Sarstedt, Nümbrecht, Germany) for 10 s, the liquid phase was discarded. The tubes were initially flash-frozen in liquid nitrogen and stored at −80 °C until RNA extraction.
4
Cultivation of P. tricornutum microalga
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The green microalga P. tricornutum was maintained at 16 °C on F/2 agar plates containing tetracycline (20 µg/mL) to eliminate bacterial contamination. Once new colonies appeared on the plate, they were streaked on new agar plates without antibiotic. Initially, the microalga was grown photoautotrophically in a photobioreactor (Multi-Cultivator MC 1000-OD; Photon Systems Instruments, Drasov, Czech Republic) on F/2 marine water enrichment solution and sodium nitrate as inorganic nitrogen source under 14/10-h light/dark regimen (intensity of 100 μmol/m2/s1) at 20 ± 1 °C. Salinity and pH were adjusted to 28 ppt and 8.0 prior to sterilization, respectively, and aeration was provided by air bubbling at atmospheric pressure. The F/2 marine water enrichment solution had the following composition (mg per liter): NaNO3, 75; NaH2PO4, 4.411; FeCl3. 6H2O, 3.15; CuSO4.5H2O, 0.01; ZnSO4.7H2O, 0.022; MnCl2.4H2O, 0.18; Na2MoO4.2H2O, 0.006; CoCl2.6H2O, 0.01; biotin, 0.005; EDTA disodium.2H2O, 4.36; thiamine.HCl, 0.1; vitamin B12, 0.005 mg. The culture conditions that were used in this study, such as light intensity, salinity, temperature, and pH, were optimized earlier for the maximum biomass and lipid accumulation by Qiao et al. (2016) [44 (link)].
5
Inducible Protein Expression in Se7942
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Se7942 was cultured in BG11 medium in a Multi-cultivator MC 1000-OD (Photon Systems Instruments, Drasov, Czech Republic) with cool white light (60 µmol photons m−2 s−1) at 30 °C and 3% CO2. From solid BG11 agar medium, individual colonies were inoculated into 60 ml of BG11 and grown for 5–7 days to be used as inocula. Cultures were inoculated at initial OD720 = 0.05 in 60 ml of fresh BG11 medium (without antibiotics). At OD720 0.5–0.6, the temperature was decreased from 30 °C to 22 °C, IPTG (Ptrc promoter) or NiSO4 (PnrsB promoter) was added to 1 mM or 5 µM final concentrations, respectively. Samples were taken 24 h post-induction.
6
Optimization of Algal Growth Conditions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chlamydomonas sp. MACC-216 was grown in TAP-N5 and TAP-N10 media under various combinations of light colors (or wavelengths) and light intensities. In total, 12 light conditions made up of combinations of three light colors (blue, red, and white) and three light intensities (50 µmol m−2 s−1, 100 µmol m−2 s−1, and 250 µmol m−2 s−1) were selected to cultivate Chlamydomonas sp. MACC-216 (Table 1 ). Chlamydomonas sp. MACC-216 was grown under each light condition in Multi-Cultivator MC-1000-OD (Photon Systems Instruments) with a continuous light source for 70 h. The absorbance was automatically measured at 720 nm at a gap of 10 h each.
For SWW, only four light conditions were selected namely, Blue 250, Blue 125 + Red 125, Red 250, and White 250 (Table 1 ) and the absorbance was measured at 720 nm similarly as mentioned above. Furthermore, Chlorella sp. MACC-38 and Chlorella sp. MACC-360 were also grown under the above-mentioned conditions to examine their growth and nitrate removal capacity in SWW.
For SWW, only four light conditions were selected namely, Blue 250, Blue 125 + Red 125, Red 250, and White 250 (
7
Exploring Iron Deficiency in Cyanobacteria
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Synechococcus elongatus PCC7942 (Syn7942) was cultivated in Multi-Cultivator MC 1000-OD (Photon Systems Instruments) with BG11 or iron-free BG11 medium at 30°C. The culture was bubbled with air and illuminated with constant cool white LEDs (Moderate light (ML): 40 µmol photons⋅m -2 ⋅s -1 ; high light (HL): 300 µmol photons⋅m -2 ⋅s -1 ) [27] (link)[28] (link)[29] . Iron-free BG11 was prepared as described 87 (link) . Iron-deficiency treatment of cells was performed as reported previously 88 (link) with a slight modification. Cells from the mid-logarithmic growth phase iron-replete cultures were pelleted at room temperature, resuspended, and washed sequentially four times with iron-free BG11. The cultures were then resuspended in iron-free BG-11 to OD 750 = 0.2 and grown under ML.
The isiA gene (Synpcc7942_1542) was deleted by replacing the isiA gene with spectinomycin resistance cassette following the Redirect strategy 89 based on homologous recombination 90 (link) . Primers used in this work were listed in Supplementary Table 3. To grow the mutant cells, BG-11 medium was supplemented with spectinomycin at 25 µg⋅mL -1 . Segregation of the mutation was confirmed by PCR (Supplementary Fig. 9).
The isiA gene (Synpcc7942_1542) was deleted by replacing the isiA gene with spectinomycin resistance cassette following the Redirect strategy 89 based on homologous recombination 90 (link) . Primers used in this work were listed in Supplementary Table 3. To grow the mutant cells, BG-11 medium was supplemented with spectinomycin at 25 µg⋅mL -1 . Segregation of the mutation was confirmed by PCR (Supplementary Fig. 9).
8
Aerobic and Anoxic Growth Curves
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Aerobic growth curves were obtained using Multi-Cultivator MC 1000-OD (Photon Systems Instruments). Cells were inoculated to 0.1 A680nm and cultivated under 27 °C, 180 µmol photons m−2 s−1 light with constant air bubbling. Cell density was measured as A680nm in 10-min intervals. Anoxic growth was measured in 1-min intervals using Hamilton Dencytee sensor (880 nm) attached to the BlueSens bioreactor.
9
Norflurazon Response in Microalgae Cultures
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
WT and mutant (M1 and M2) cultures, grown to mid-log phase, were diluted to a chlorophyll concentration of 2 mg L−1 and cultivated in cylindrical glass columns in a Multi-Cultivator MC 1000-OD (Photon Systems Instruments, Czech Republic) with different norflurazon concentrations (0, 5 and 10 μM) at 22 °C, a light intensity of 50 μmol photons m−2 s−1, and bubbling with filtered air enriched with 2% CO2. Chlorophyll and cell density were recorded in situ, every 10 minutes for 93 h, via optical density measurements, at 680 nm and 720 nm, respectively. The experiment was repeated three times, and the average values are reported. At the end of the experiment, cells were observed microscopically under a bright field and were also stained with Aniline Blue to assess cellular damage.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!