B6 cg tg camk2a cre t29 1stl j

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B6.Cg-Tg(Camk2a-cre)T29-1Stl/J is a transgenic mouse strain. It expresses Cre recombinase under the control of the calcium/calmodulin-dependent protein kinase II alpha (Camk2a) promoter.

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19 protocols using b6 cg tg camk2a cre t29 1stl j

Genetically Modified Mouse Strains

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Mice were housed in the Center for Comparative Physiology at the Feinstein Institutes for Medical Research. C57BL/6 (B6) and B6.C-H2d/bByJ (B6.H2^d) mice were purchased from The Jackson Laboratory. RAGE KO mice were a generous gift from Kevin Tracey, MD, of the Feinstein Institutes for Medical Research. LAIR-1 cKO mice were generated by crossing B6.129P2-Lyz2^tm1(cre)Ifo/J with B6.Cg-Lair1^tm1Jco/J (The Jackson Laboratory) after backcrossing onto the B6.H2^d background. B6.Cg-Tg(Camk2a-cre)T29–1Stl/J and Hmgb1^tm1.1Rshw/J (Jackson Laboratories) were crossed with in house B6.H2^d mice to generate Camk2a-cre HMGB1^fl/fl B6.H2^d mice. All procedures using mice were conducted using protocols approved by The Feinstein Institutes of Medical Research IACUC (Protocols 2009–048 and 2022–023).

Carroll K.R., Mizrachi M., Simmons S., Toz B., Wingard J., Tehrani N., Zarfeshani A., Kello N., Kowal C., Levin J.Z., Volpe B.T, & Diamond B. (2023). Lupus autoantibodies initiate a maladaptive equilibrium sustained by HMGB1:RAGE signaling and reversed by LAIR-1:C1q signaling. Research Square.

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Transgenic Mouse Lines for Neurobiological Research

Cited 6 times

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Animal use was approved and overseen by the Harvard University Institutional Animal Care and Use Committee and the Harvard Center for Comparative Medicine. The following mouse lines were used: wild-type C57/BL6 (Jackson Laboratory, 000664); Npas4^fl/fl^{14 (link)}; Npas4-knockout^{14 (link)}; Tip60^fl/fl^{49 (link)}; Npas4–Flag-HA (this manuscript); Arnt2–Flag-HA (this manuscript), Tip60-H3F^{50 (link)}; Mre11^fl/fl^{51 (link),52 (link)}; B6;129-Gt(ROSA)26Sor<tm5(CAG-Sun1/sfGFP)Nat>/J (Jackson Laboratory, 021039)^{53 (link)}; and B6.Cg-Tg(Camk2a-cre)T29-1Stl/J (Jackson Laboratory, 005329)^{54 (link)}. Mice were housed in a temperature and humidity-controlled environment using ventilated microisolator cages. Mice were kept under a standard 12 h light–dark cycle, with food and water provided ad libitum. Male and female littermate mice were used in similar proportions and divided between control and experimental groups for all experiments conducted. No statistical methods were used to predetermine sample sizes. For biochemistry and genomic experiments, animals were collected at 4–6 weeks of age throughout the manuscript. For physiology experiments, animals were dissected and patched at postnatal day 24 (P24) to P28. For ageing experiments, animals were collected at 3-4 months, 12 months and 23–27 months of age. Details of animal age and sex are detailed within each protocol.

Pollina E.A., Gilliam D.T., Landau A.T., Lin C., Pajarillo N., Davis C.P., Harmin D.A., Yap E.L., Vogel I.R., Griffith E.C., Nagy M.A., Ling E., Duffy E.E., Sabatini B.L., Weitz C.J, & Greenberg M.E. (2023). A NPAS4–NuA4 complex couples synaptic activity to DNA repair. Nature, 614(7949), 732-741.

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Genetic Mouse Models for Neuroscience

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All experiments were approved by and performed in agreement with the guidelines of the Institutional Animal Care and Use Committee (IACUC) at University of Colorado School of Medicine. 6 - 8 week-old mice used in experiments were: CaMKII-Cre heterozygote mice (generated from WT and homozygous B6.Cg-Tg(Camk2a-cre)T29-1Stl/J, Jackson Laboratory), A2a-cre mice (Tg(Adora2a-cre) KG139Gsat; MMRRC), ChAT-IRES-Cre mice (Chat^{tim2(cre)Lowl}/J, Jackson Laboratory) and wild-type C57BL/6J mice (Jackson Laboratory). Both male and female mice were used for all experiments.

Mamaligas A.A., Barcomb K, & Ford C.P. (2019). Cholinergic Transmission at Muscarinic Synapses in the Striatum Is Driven Equally by Cortical and Thalamic Inputs. Cell reports, 28(4), 1003-1014.e3.

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Genetic Manipulation of Mouse Behavior

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Both male and female adult (3–6 months old) wildtype (C57BL/6J), DAT-cre [B6.SJL-Slc6a3tm1.1(cre)Bkmn/J, Jackson Laboratory], and CaMKIIa-cre [B6.Cg-Tg(Camk2a-cre)T29-1Stl/J, Jackson Laboratory] mice were included in this study. Mice were housed on a 12 h light/dark cycle and provided ad libitum access to water and food. Prior to stereotaxic surgical injection, mice were housed in groups (2–4 per cage). Littermates were randomly assigned to experimental groups or control groups. Following the surgical procedure, mice were subsequently single caged until all experimental procedures were finished. Mice were handled daily for 2 weeks prior to behavioral tests, which were conducted during the light cycle. All experiments were carried out in accordance with Florida State University’s laboratory animal care and use guidelines and approved by the Florida State University Animal Care and Use Committee.

Zhang J., Navarrete M., Wu Y, & Zhou Y. (2022). 14-3-3 Dysfunction in Dorsal Hippocampus CA1 (dCA1) Induces Psychomotor Behavior via a dCA1-Lateral Septum-Ventral Tegmental Area Pathway. Frontiers in Molecular Neuroscience, 15, 817227.

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Ultrasonic Vocalization in Transgenic Mice

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We used C57BL/6 male mice unless otherwise noted (at least 8 weeks old). In addition, we used several genetically modified mouse lines (Camk2, Rbp4, Thy1, Rbp4-Ai32, and Pv-CRE): (B6.Cg-Tg(Camk2a-cre)T29-1Stl/J from the Jackson Laboratory, Tg(Rbp4-cre) from the Jackson Laboratory, B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J from the Jackson Laboratory, B6;129S-Gt(ROSA)26Sortm32(CAG-COP4*H134R/EYFP)Hze/J from the Jackson Laboratory crossed with RBP4 mice as above, and B6;129P2-Pvalbtm1(cre)Arbr/J from the Jackson Laboratory. The Hebrew University Animal Care and Use Committee approved all experiments (IACUC NS-16-14216-3, “Functional identification of brain regions involved in ultrasonic vocalization in mice.”).

Gan-Or B, & London M. (2023). Cortical circuits modulate mouse social vocalizations. Science Advances, 9(39), eade6992.

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Optogenetic Manipulation of ACC Neurons

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Channelrhodopsin 2 (ChR2) was injected bilaterally as described on a CamKII promotor in the AAV9 viral serotype into transgenic mice expressing CRE in CamkII-positive neurons [B6.Cg-Tg(Camk2a-cre)T29-1Stl/J from the Jackson Laboratory]. Fiber optical cannulas (multimode fibers FT-200-URT, Thorlabs, Grünberg, Germany) with a diameter of 200 μm and a NA of 0.22 were implanted at a 10° angle into ACC at coordinates (700 lateral, 700 anterior) and covered in dental cement. After recovery and 2 weeks to allow for viral expression, mice were tested while exploring either their home cage or a fresh cage with no female present before or during the session. Light was delivered through a 100-mW laser (1.5 to 3 mW/mm² using a 473-nm laser; Changchun New Industries Optoelectronics, Changchun, China) attached to a rotary joint splitter (Part # RJ2, Thorlabs, Grünberg, Germany) to allow for free exploration. ACC neurons were activated with 10 s of stimulation with about 30 s of rest between stimulations. Stimulus trains consisted of 20-Hz pulses of 10 ms.

Gan-Or B, & London M. (2023). Cortical circuits modulate mouse social vocalizations. Science Advances, 9(39), eade6992.

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Generation of PERK cKO Mice

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All mice are housed in a barrier facility dedicated to transgenic mice at Wake Forest School of Medicine. Randomization was not used in animal studies. The facility operates in accordance with standards and policies of the US Department of Agriculture’s Animal Welfare Information Center (AWIC), and the NIH Guide for Care and Use of Laboratory Animals. The facility is kept on a 12 h light/dark cycle, with a regular feeding and cage-cleaning schedule. Mice of both sexes were used. The following mice were purchased from the Jackson Laboratory (Bar Harbor, ME): B6.Cg-Tg (Camk2a-cre)T29-1Stl/J (Camk2a-cre mice), stock No. 005359; Prkaa1tm1.1Sjm/J (loxP-flanked Prkaa1 mice), stock No. 014141; Prkaa2tm1.1Sjm/J (loxP-flanked Prkaa2 mice), stock No. 014142. Generation of PERK cKO mice was as described [27 (link)]. The genotypes were verified by polymerase chain reaction (PCR). Mice of 3–6-month old were used for all experiments. Investigators were not blind to the group allocation during the experiments.

Yang W., Zhou X., Zimmermann H.R, & Ma T. (2020). Brain-specific suppression of AMPKα2 isoform impairs cognition and hippocampal LTP by PERK-mediated eIF2α phosphorylation. Molecular psychiatry, 26(6), 1880-1897.

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Conditional Girk1 Knockout in Mice

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Animal experiments were approved by the University of Minnesota Institutional Animal Care and Use Committee. CaMKIICre [B6.Cg-Tg(Camk2a-cre)T29-1Stl/J] and GADCre (B6N.Cg-Gad2^tm2(cre)Zjh/J) lines were purchased from The Jackson Laboratory, and have been maintained by backcrossing against the C57BL/6J strain. Offspring from these crosses were used to generate the Cre(+) and Cre(−) mice used in this study. The generation of conditional CaMKIICre(+):Girk1^fl/fl mice was described previously (Marron Fernandez de Velasco et al., 2017 (link)). Unless specifically noted, males and females were used in all experiments, and groups were balanced by sex. All mice were maintained on a 12 h light/dark cycle, and were provided ad libitum access to food and water.

Tipps M., Marron Fernandez de Velasco E., Schaeffer A, & Wickman K. (2018). Inhibition of Pyramidal Neurons in the Basal Amygdala Promotes Fear Learning. eNeuro, 5(5), ENEURO.0272-18.2018.

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Genetic Mouse Models for Neuroscience

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Mamaligas A.A., Barcomb K, & Ford C.P. (2019). Cholinergic Transmission at Muscarinic Synapses in the Striatum Is Driven Equally by Cortical and Thalamic Inputs. Cell reports, 28(4), 1003-1014.e3.

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CaMKIIα-Cre Mouse Behavioral Studies

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All methods were in accordance with the guidelines of and approved by the Institutional Animal Care and Use Committee (IACUC Approval #00155) at the University of Colorado Anschutz Medical Campus. For all experiments, heterozygous CaMKIIα-Cre mice were used (Tsien et al., 1996 (link)). Animals were bred in house between homozygous CaMKIIα-Cre (B6.Cg-Tg(Camk2a-cre)T29–1Stl/J; Jackson Labs, Stock No. 005359) and C57/BL6J (Jackson Labs, Stock No. 000664). Mice were maintained on a 12 hour light:dark cycle and were provided access to food and water ad libitum. Male and female mice were used for all experiments.

Barcomb K., Olah S.S., Kennedy M.J, & Ford C.P. (2022). Properties and modulation of excitatory inputs to the locus coeruleus. The Journal of Physiology, 600(22), 4897-4916.

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