Human lncRNA Microarray V3.0 was manufactured by Arraystar Inc (MD, USA) and covered more than 40,000 lncRNAs and 20,000 mRNAs in the human genome. The microarray hybridization, collection of expression data and analysis of microarray data were performed by KangChen Bio-tech, Shanghai, China.
Microarray v3
Manufactured by Arraystar
Sourced in United States
Microarray V3.0 is a high-throughput gene expression analysis platform developed by Arraystar. It is designed to detect and quantify the expression of thousands of genes simultaneously in a single experiment. The core function of the Microarray V3.0 is to provide researchers with a comprehensive and efficient tool for transcriptome analysis.
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Lab products found in correlation
Trizol rna extraction kit, by Thermo Fisher Scientific (1 mentions)
Flash rna labeling kit, by Arraystar (1 mentions)
Mrna only eukaryotic mrna isolation kit, by Illumina (1 mentions)
Agilent feature extraction software version 11.0.1.1, by Agilent Technologies (1 mentions)
Agilent dna microarray scanner part number g2505c, by Agilent Technologies (1 mentions)
3 protocols using microarray v3
1
Human lncRNA Microarray Protocol
2
Transcriptome Analysis of Choroidal Neovascularization
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Tissues of RPE-choroid-sclera complexes were collected from 4 eyes to be pooled as one sample, and a total of 6 samples (3 CNV samples and 3 control samples) were analyzed. Total RNA was isolated by using Trizol RNA extraction kit (Invitrogen, Carlsbad, CA, USA). The microarray analysis was performed by mouse lncRNA Microarray V3.0 (Arraystar, Rockville, MD, USA) as described 20 (link). The raw data of microarray has been uploaded to the Gene Expression Omnibus database (http://www.ncbi.nlm.nih.gov/geo/ ) for public access with the accession number GSE129743. Altered lncRNAs and mRNAs expression were identified by a Volcano Plot filtering [fold change (FC) ≥1.5] and P<0.05.
3
Transcriptomic Analysis of TNBC Cell Lines
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MDA-MB-231 and Hs578T cells were treated with 100 n M of DHT for 48 hours. The total RNA was extracted from treated or untreated TNBC cells and mRNA was purified from total RNA after removal of rRNA (mRNA-ONLY™ Eukaryotic mRNA Isolation Kit, Epicentre). Then, each sample was amplified and transcribed into fluorescent cRNA along the entire length of the transcripts without 3' bias utilizing a random priming method (Arraystar Flash RNA Labeling Kit, Arraystar). The labeled cRNA was then hybridized onto the Microarray v3.0 (ArrayStar). After the washing steps, the Agilent DNA Microarray Scanner (part number G2505C) was used to scan the arrays. Acquired array images were analyzed by Agilent Feature Extraction software (version 11.0.1.1). Quantile normalization and subsequent data processing were performed using The Agilent GeneSpring GX v12.1 software package (Agilent Technologies). The threshold we used to screen dysregulated mRNAs is fold-change ≥ 2.0 and a P-value ≤ 0.05.
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