All participants underwent an 8-h fasting period prior to the collection of blood samples from the antecubital vein. The same machine in a single laboratory was used to analyze various indicators in the collected samples. These indicators included fasting blood glucose, HbA1c, creatinine, and 25(OH)D levels. Serum 25(OH)D concentrations were measured using the chemiluminescent microparticle immunoassay (CMIA) using an Architect i2000SR system (Abbott, Singapore). The TNF-α, IL-6, and IL-1β concentrations in the serum were quantified using the Human Luminex® Discovery assay kit (R&D Systems, Inc., Minneapolis, MN, USA) as per the manufacturer’s instructions [20 (link)]. The estimated glomerular filtration rate (eGFR) of the kidneys was calculated using the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) equation [21 (link)].
Human luminex discovery assay kit
Manufactured by R&D Systems
Sourced in United States
The Human Luminex® Discovery assay kit is a multiplex system that allows for the simultaneous detection and quantification of multiple analytes in a single sample. The core function of this kit is to provide researchers with a powerful tool for analyzing complex biological samples, enabling a more comprehensive understanding of various biological processes.
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Lab products found in correlation
2 protocols using human luminex discovery assay kit
1
Biomarkers of Metabolic and Inflammatory Status
2
Cytokine Profiling in Cancer Cell Lines
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HT-29, Colo-205, and HCT-116 cells were seeded into 24-well plates (2.5 × 105 cells per well in 500-μL serum-free medium). Cells were treated with a single compound (vemurafenib, axitinib, or pyrvinium), combination of vemurafenib and axitinib, or combination of vemurafenib and pyrvinium for 12 hours. Culture supernatants were collected and centrifuged at 1,000 × g for 5 minutes to remove cell debris. Supernatants were assayed for VEGF-A using a VEGF-A Human ProcartaPlex Simplex Kit (Invitrogen) and for MIF, IL8, and TGFα using a Human Luminex Discovery Assay KIT (R&D Systems) following the manufacturers’ instructions. Data were normalized by cell counts relative to the control wells.
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