Snu886

Manufactured by Korean Cell Line Bank

The SNU886 is a laboratory equipment product. It is designed for the cultivation and maintenance of cell lines. The core function of the SNU886 is to provide a controlled environment for cell growth and proliferation.

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5 protocols using snu886

Comparative Cell Line Characterization

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C3A, HepG2, SNU398, PLC/PRF/5, Hep3B2.1-7, SKHep1, SNU475, SNU387, SNU423 and SNU449 cell lines were purchased from the American Type Culture Collection (ATCC). NCI-H684, SNU761, SNU886 and SNU878 were purchased from the Korean Cell Line Bank (KCLB). JHH4, JHH6 and HuH-7 cells were purchased form the JRCB Cell Bank. FOCUS cells were obtained from the Laboratory of J. Wands, Brown University (He et al., 1984 (link)). All cells were grown at 37°C under 5% CO2, 95% ambient atmosphere. Fifteen cryofrozen cell stocks were generated from the original vial from the cell bank (Passage 3). Experiments were performed with cells at <10 passages from the original vial. All cell media used were supplemented with 100x penicillin-streptomycin-glutamine (Thermo Fisher Scientific, Waltham, MA). FOCUS and HuH-7 cells were grown in Dulbecco’s minimum essential medium (DMEM) supplemented with 10% FBS (VWR Life Science, Seradigm). C3A, HepG2, SNU398, PLC/PRF/5, Hep3B2.1-7, SKHep1, SNU475, SNU387, SNU423 and SNU449 were grown in the ATCC-recommended medium. JHH4 cells were grown in Eagle’s minimum essential medium (MEM), JHH6 cells in William’s E medium and NCI-H684, SNU761, SNU886 and SNU878 in RPMI 1640 medium all supplemented with 10% FBS. Cells were harvested when reaching 90% confluency.

Golkowski M., Lau H.T., Chan M., Kenerson H., Vidadala V.N., Shoemaker A., Maly D.J., Yeung R.S., Gujral T.S, & Ong S.E. (2020). Pharmacoproteomics identifies kinase pathways that drive the epithelial-mesenchymal transition and drug resistance in hepatocellular carcinoma. Cell systems, 11(2), 196-207.e7.

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Comprehensive Cancer Cell Line Profiling

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A549, BT-20, BT-474, BT-549, CAMA-1, DMS-53, DU4475, HCC38, HCC70, HCC202, HCC1143, HCC1187, HCC1395, HCC1569, HCC1806, HCC1937, HCC1954, HCC2218, HCT-116, Hs578T, Jeko-1, MCF-7, MDA-MB-134-VI, MDA-MB-157, MDA-MB-175-VII, MDA-MB-231, MDA-MB-361, MDA-MB-415, MDA-MB-436, MDA-MB-453, MDA-MB-468, MiaPaCa2, SK-BR-3, NCI-H441, SK-MEL-28, T-47D, U2OS, and ZR-75-1 were obtained from the American Type Culture Collection (ATCC) and cultured according to vendor recommendations. EFM-19 were obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ) and SNU-886 were from the Korean Cell Line Bank (KCLB).
Abemaciclib, palbociclib, ribociclib, PIM447, BYL719, LY2090314, everolimus, DYRK1Bi AZ cpd 33 [32 (link)], dinaciclib, GSK2334470, abemaciclib metabolites M2 and M20 [28 (link)], and additional CDK4/6i (see Figure 2C [33 ],) were synthesized by Lilly Research Laboratories. AZD1208 (S7104) and additional palbociclib (S1579, see Supplementary Figure 1A) were purchased from Selleck Chemicals.

Litchfield L.M., Boehnke K., Brahmachary M., Mur C., Bi C., Stephens J.R., Sauder J.M., Gutiérrez S.M., McNulty A.M., Ye X.S., Wu W., Lallena M.J., Gong X., Merzoug F.F., Jansen V.M, & Buchanan S.G. (2020). Combined inhibition of PIM and CDK4/6 suppresses both mTOR signaling and Rb phosphorylation and potentiates PI3K inhibition in cancer cells. Oncotarget, 11(17), 1478-1492.

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Culture of Hepatocellular Carcinoma Cell Lines

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Six HCC cell lines, HepG2, SNU-449, SNU-475, SNU-761, SNU-878 and SNU-886, were purchased from the Korean Cell-Line Bank (Seoul, Korea) and were maintained in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum at 37 °C under 5% CO₂. The THLE-2 cell line, a human normal liver cell line, was purchased from ATCC (Manassas, VA, USA) and maintained in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum, 25 mM HEPES buffer and 100 U ml⁻¹ penicillin.

Xu H., Choe C., Shin S.H., Park S.W., Kim H.S., Jung S.H., Yim S.H., Kim T.M, & Chung Y.J. (2014). Silencing of KIF14 interferes with cell cycle progression and cytokinesis by blocking the p27Kip1 ubiquitination pathway in hepatocellular carcinoma. Experimental & Molecular Medicine, 46(5), e97-.

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Cell Line Characterization and Compound Screening

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The THLE2, THLE3, Huh7, SK‐HEP‐1 and PLC/PRC/5 cells were got from American Type Culture Collection, HLE, HLF and Huh1 cells were got from Japanese Collection of Research Bioresources, SNU886, SNU878, SNU761 and SNU739 cells were got from Korean Cell Line Bank, and MHCC‐97H cells were got from the National Collection of Authenticated Cell Cultures (Shanghai, China). Cell lines were determined using MycoBlue Mycoplasma Detection Kit (Vazyme, D101‐01) and confirmed to be free of mycoplasma contamination. All cells were cultured in corresponding medium with 10% fetal bovine serum (FBS; Gbico). The medium were listed as follow: Dulbecco's modified Eagle's medium (DMEM) for MHCC‐97H, Huh1, Huh7, HLE and HLF; Roswell Park Memorial Institute (RPMI) 1640 for SNU761, SNU886, SNU878 and SNU739; Eagle's Minimum Essential Medium (EMEM) for SK‐HEP‐1 and PLC/PRC/5; BEGM (Lonza; CC‐3170) for THLE2 and THLE3. The PLK1 inhibitors BI2536 (S1109) and NMS‐P937 (S7255) as well as the Smad3 inhibitor SIS3 (S7959) were got from Selleck Chemicals. Blasticidin S was got from Beyotime. Puromycin and doxycline (Dox) was purchased from ThermoFisher.

Tang Q., Hu G., Sang Y., Chen Y., Wei G., Zhu M., Chen M., Li S., Liu R, & Peng Z. (2024). Therapeutic targeting of PLK1 in TERT promoter‐mutant hepatocellular carcinoma. Clinical and Translational Medicine, 14(5), e1703.

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Liver Cancer Cell Line Cultivation

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HepG2, Hep3B, SNU398, and SNU423 cells were obtained from the American Type Culture Collection (Manassas, VA). HLE, HLF, Huh1, Huh7, JHH2, JHH4, JHH5, JHH6, and JHH7 cells were purchased from the Japanese Collection of Research Bioresources Cell Bank in Japan. SNU368 and SNU886 were obtained from the Korean Cell Line Bank in Korea. All cells were tested for mycoplasma before use. The cell lines were cultured in the recommended medium supplemented with 10% heat-inactivated fetal bovine serum (FBS) and penicillin-streptomycin in a 5% CO₂ humidified incubator at 37°C.

Kim Y., Jo M., Schmidt J., Luo X., Prakash T.P., Zhou T., Klein S., Xiao X., Post N., Yin Z, & MacLeod A.R. (2019). Enhanced Potency of GalNAc-Conjugated Antisense Oligonucleotides in Hepatocellular Cancer Models. Molecular Therapy, 27(9), 1547-1557.

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