Irdye tagged secondary antibodies

Modified Eagle’s medium (MEM), fetal bovine serum (FBS), L-glutamine, MitoSOX for reactive oxygen species (ROS) assay, IRDye-tagged secondary antibodies, Hoechst nuclear stain, penicillin, streptomycin, and other cell culture reagents were purchased from Invitrogen (Carlsbad, CA, USA). ATF-4, p-IRE1α, p-eIF2α, PKCδ, and p-PKCδ Y311 antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). A comprehensive list of the antibodies used in this study has been provided in Supplementary Table 1. NLRP3, ASC, and caspase-1 antibodies were purchased from AdipoGen (San Diego, CA, USA). The TrX and TXNIP antibody purchased from the Abcam (Cambridge MA). The β-actin antibody was purchased from Sigma-Aldrich (St. Louis, MO, USA). Mito-apocynin (MitoApo) was obtained from Dr. Kalyanaraman (Medical College of Wisconsin, Milwaukee, WI, USA). Protein A/G magnetic beads were purchased from Thermo Fisher Scientific (Waltham, MA). The CD11b magnetic separation kit was purchased from Stem Cell Technologies (Vancouver, Canada). The Duo-link proximity ligation assay reagents were purchased from Sigma Aldrich (St. Louis, MO). The Bradford protein assay was purchased from Bio-Rad Laboratories (Hercules, CA). The quick western kit was purchased from the LICOR (Lincoln, NE, USA).

Recombinant TWEAK was obtained from PeproTech. Modified Eagle’s medium (MEM), fetal bovine serum (FBS), L-glutamine, MitoSox, DCFDA-H2 kit for ROS assay, IRDye-tagged secondary antibodies, Hoechst nuclear stain, penicillin, streptomycin and other cell culture reagents were purchased from Invitrogen (Carlsbad, CA, USA). ELISA kits for IL-1β, IL-18, TNF-α and TWEAK were purchased from RayBiotech (Peachtree Corners, GA, USA). Rabbit anti-NLRC4, PKCδ, pPKCδ-Y311, STAT3 and pSTAT3 antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). ASC and caspase-1 antibodies were purchased from AdipoGen (San Diego, CA, USA). GFAP antibody was purchased from EMD Millipore (Burlington, MA, USA). Aurintricarboxylic acid (ATA), 1-methyl-4-phenylpyridinium (MPP⁺ iodide), MPTP-HCl, and β-actin antibody were purchased from Sigma-Aldrich (St. Louis, MO, USA). Mito-apocynin was obtained from Dr. Kalyanaraman (Medical College of Wisconsin, Milwaukee, WI, USA). MitoTracker Red CMXRos was purchased from Molecular Probes (Eugene, OR, USA). Luminex multiplex cytokine (Multiplex mouse cytokine panel) assay kit was purchased from Bio-Rad Laboratories (Hercules, CA, USA). The CD11b magnetic separation kit was purchased from Stem Cell Technologies (Vancouver, Canada). The Bradford protein assay and TidyBlot detection reagents were purchased from Bio-Rad Laboratories.