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Ham s f 12 modified essential eagle s media

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Ham's F-12 modified essential Eagle's media is a cell culture medium used to support the growth and maintenance of various cell lines. It provides the necessary nutrients and components for cell proliferation and survival in an in vitro environment.

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Lab products found in correlation

Alpha minimal essential medium, by Thermo Fisher Scientific (2 mentions) Dulbecco s modified eagle s media, by Thermo Fisher Scientific (2 mentions) Ht1080, by American Type Culture Collection (2 mentions)

Close spelling variants of this product

F-12 Ham

3 protocols using ham s f 12 modified essential eagle s media

1

Culturing Diverse Cell Lines for Research

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
The human fibrosarcoma cell line HT1080 (CCL-121) was purchased from the American Type Culture Collection, and human embryonic kidney cells expressing the large-T antigen (HEK293T) were a kind gift from Dr. Ashis Basu (University of Connecticut). Human cells were cultured in Dulbecco’s modified Eagle’s media (Life Technologies, Grand Island, NY) supplemented with 9% fetal bovine serum (Atlanta Biologics, Atlanta, GA). Chinese hamster AA8 51D1 cells were a kind gift from Professor Claudia Wiese (Colorado State University) in which the Rad51D gene was knocked out using a gene-targeting vector [55 (link)]. Rad51D is a paralog of Rad51 which contributes to the HR pathway in vertebrates [56 (link)]. These cells were cultured in alpha minimal essential medium (Life Technologies, Grand Island, NY) supplemented with 9% fetal bovine serum. Chinese hamster lung fibroblast cell line V79 (GM16136) were obtained from the Coriell Institute for Medical Research (Camden, NJ) and cultured in Ham’s F-12 modified essential Eagle’s media (Life Technologies, Grand Island, NY) supplemented with 9% fetal bovine serum. All cells were maintained in a humidified atmosphere of 5% carbon dioxide, 95 % air, at 37 °C.
Chesner L.N., Essawy M., Warner C, & Campbell C. (2020). DNA-protein crosslinks are repaired via homologous recombination in mammalian mitochondria. DNA repair, 97, 103026.
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2

Culturing Diverse Cell Lines for Research

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
The human fibrosarcoma cell line HT1080 (CCL-121) was purchased from the American Type Culture Collection, and human embryonic kidney cells expressing the large-T antigen (HEK293T) were a kind gift from Dr. Ashis Basu (University of Connecticut). Human cells were cultured in Dulbecco’s modified Eagle’s media (Life Technologies, Grand Island, NY) supplemented with 9% fetal bovine serum (Atlanta Biologics, Atlanta, GA). Chinese hamster AA8 51D1 cells were a kind gift from Professor Claudia Wiese (Colorado State University) in which the Rad51D gene was knocked out using a gene-targeting vector [55 (link)]. Rad51D is a paralog of Rad51 which contributes to the HR pathway in vertebrates [56 (link)]. These cells were cultured in alpha minimal essential medium (Life Technologies, Grand Island, NY) supplemented with 9% fetal bovine serum. Chinese hamster lung fibroblast cell line V79 (GM16136) were obtained from the Coriell Institute for Medical Research (Camden, NJ) and cultured in Ham’s F-12 modified essential Eagle’s media (Life Technologies, Grand Island, NY) supplemented with 9% fetal bovine serum. All cells were maintained in a humidified atmosphere of 5% carbon dioxide, 95 % air, at 37 °C.
Chesner L.N., Essawy M., Warner C, & Campbell C. (2020). DNA-protein crosslinks are repaired via homologous recombination in mammalian mitochondria. DNA repair, 97, 103026.
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3

Culturing Chinese Hamster Lung Fibroblasts

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Chinese hamster lung fibroblast cell lines V79 (GM16136) were obtained from the Coriell Institute for Medical Research (Camden NJ). Cells were grown to 80–90% confluence on tissue culture dishes in Ham’s F-12 modified essential Eagle’s media (Life Technologies, Grand Island, NY, USA) supplemented with 9% fetal bovine serum. Cells were maintained in a humidified atmosphere of 5% carbon dioxide, 95% air, at 37 °C.
Wickramaratne S., Banda D., Ji S., Manlove A.H., Malayappan B., Nuñez N.N., Samson L., Campbell C., David S, & Tretyakova N. (2016). Base Excision Repair of N6-Deoxyadenosine Adducts of 1,3-Butadiene. Biochemistry, 55(43), 6070-6081.
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