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Goat anti synaptophysin

Manufactured by Santa Cruz Biotechnology
Sourced in Germany

Goat anti-synaptophysin is a primary antibody that binds to the synaptophysin protein. Synaptophysin is a membrane glycoprotein found in presynaptic vesicles and is commonly used as a marker for synapses.

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2 protocols using goat anti synaptophysin

1

Antibody Use for Neuronal Protein Analysis

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Rabbit and rat antibodies to VAPB and PTPIP51 were as described [9 (link)]. Rabbit anti-PTPIP51 and chicken anti-MAP 2 were from Gentex. Rabbit anti-translocase of the outer mitochondrial membrane protein-20 (TOM20), mouse anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and goat anti-synaptophysin were from Santa Cruz Biotechnology. Mouse anti-β-Tubulin Isotype III antibody and mouse anti-β-Actin were from Sigma. Mouse anti-post synaptic density protein-95 (PSD95) was from Millipore, mouse anti-protein disulphide isomerase (PDI) was from Thermo Fisher Scientific and mouse anti-phosphorylated neurofilament heavy chain (NFH) (antibody SMI31) was from Sternberger Monoclonals Inc. Species specific goat and donkey anti-mouse, −rabbit and -chicken Igs coupled to AlexaFluor-488, − 594 or − 647 were from Invitrogen, Jackson ImmunoResearch, ThermoFisher or Abcam. FM 4–64 was from Invitrogen, DL-2-amino-5-phosphonovaleric acid (AP5) was from Cayman chemical company and 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) was from Santa Cruz.
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2

Antibody Panel for Synaptic Proteins

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The primary antibodies used were anti-3-nitrotyrosine (A21285, Invitrogen, Waltham, USA), goat anti-4HNE (H6275-02, US Biological, Life Sciences, Salem, USA), mouse anti-Nrf2 (A-10) (sc-365949, Santa Cruz Biotechnology, Dallas, USA), mouse anti-PGC1α (4A8; sc-517380, Santa Cruz Biotechnology), mouse anti-Syt1/2 (H-9) (sc-393392, Santa Cruz Biotechnology), mouse anti-Syp (D-4; sc-17750, Santa Cruz Biotechnology), goat anti-pSyn Ia/b (Ser 553; sc-12913, Santa Cruz Biotechnology), mouse monoclonal anti-PSD95 (clon K28/43, UC Davis/NIH NeuroMab Facility, Davis, USA), mouse anti-GluN2A (E-4, sc-515148, Santa Cruz Biotechnology), mouse anti-GluN2B (A-8; sc-365597, Santa Cruz Biotechnology), mouse anti-GluA2 (clone L21/32; UC Davis/NIH NeuroMab Facility), goat anti-synaptophysin (sc-7568, Santa Cruz Biotechnology), and rabbit polyclonal anti-Arc (156 003, Synaptic Systems, Goettingen, Germany). Anti-Actin (sc-47778, Santa Cruz Biotechnology) or GAPDH (sc-25778, Santa Cruz Biotechnology) were used as the loading control.
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