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Ab203680

Manufactured by Abcam
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Sourced in United States

Ab203680 is a laboratory instrument used for DNA and protein analysis. It is a piece of equipment designed to perform specific tasks in a laboratory setting.

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Lab products found in correlation

Ab8245, by Abcam (1 mentions) Ab8536, by Abcam (1 mentions) Alexa fluor 568 donkey anti goat, by Thermo Fisher Scientific (1 mentions) Hrp conjugated second antibody, by Thermo Fisher Scientific (1 mentions) Mouse anti cd34, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 conjugated donkey anti rabbit, by Thermo Fisher Scientific (1 mentions) Ab1316, by Abcam (1 mentions) Ab38449, by Abcam (1 mentions) Ab214430, by Abcam (1 mentions) Ab8805, by Abcam (1 mentions) Ab205718, by Abcam (1 mentions) Ab182651, by Abcam (1 mentions) Ab182858, by Abcam (1 mentions) Ab53154, by Abcam (1 mentions) Ecl chemiluminescence reagent, by Beyotime (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Ripa lysis buffer, by Beyotime (1 mentions) Ab8227, by Abcam (1 mentions) Ab16502, by Abcam (1 mentions) Ab86299, by Abcam (1 mentions)

3 protocols using ab203680

1

Western Blot Analysis of AGGF1 and GAPDH

Cited 1 time
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The WB assays were performed as described previously [13 (link)–15 (link)]. Primary antibodies specific to AGGF1 (1:500, # ab203680) and GAPDH (1:1000, # ab8245) were purchased from Abcam (Cambridge, UK).
Zhang X., Sun H., Chen W, & He X. (2019). Elevated expression of AGGF1 predicts poor prognosis and promotes the metastasis of colorectal cancer. BMC Cancer, 19, 1252.
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2

Immunohistochemistry and Immunofluorescence of AGGF1, VEGF, and CD34

Cited 2 times
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Immunohistochemistry and immunofluorescence were performed as described in our previous study [19 (link), 20 (link)]. Primary antibodies were rabbit anti-AGGF1 (ab203680, 1:200), mouse anti-VEGF (ab1316, 1:150) and mouse anti-CD34 (ab8536, 1:400, all from Abcam, USA). The HRP-conjugated second antibody was from Invitrogen, Carlsbad, CA and the diaminobenzidine was from Beijing Zhongshan Golden Bridge Biotech, China. As for immunofluorescence, the primary antibodies cocktail and the mixture of secondary antibodies were rabbit anti-AGGF1 (1:150), mouse anti-CD34 (1:200) and Alexa Fluor 488-conjugated donkey anti-rabbit, Alexa Fluor 568 donkey anti-goat (all from Invitrogen), respectively.
For the negative control, the primary antibody was carried out with phosphate-buffered saline (PBS).
Tu J., Ying X., Zhang D., Weng Q., Mao W., Chen L., Wu X., Tu C., Ji J, & Huang Y. (2017). High expression of angiogenic factor AGGF1 is an independent prognostic factor for hepatocellular carcinoma. Oncotarget, 8(67), 111623-111630.
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3

Hippocampal Protein Extraction and Western Blot

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Briefly, RIPA lysis buffer was used to extract the total protein from hippocampus tissues (Beyotime, Shanghai, China). The BCA kit (CoWin Biotechnology) was used to determine the concentration of protein, which was then electrophoresed by SDS-PAGE. Proteins were next Physiology International transferred to PVDF membranes (Millipore, Boston, MA, USA) followed by immersion in 5% non-fat milk for 1 h. The membranes were then incubated with different specific primary antibodies at 4 8C for 12 h. The antibodies were against AGGF1 (ab203680, 1:1200; Abcam), Cleaved Caspase-3 (ab214430, 1:5000; Abcam), Bax (ab53154, 1:3000; Abcam), Bcl-2 (ab182858, 1:1200; Abcam), p-PI3K (ab182651, 1:3000; Abcam), PI3K (ab ab154598, 1:3000; Abcam), p-AKT (ab38449, 1:3000; Abcam), AKT (ab8805, 1:2200; Abcam), p-NF-kB p65 (ab86299, 1:2200; Abcam), NF-kB p65 (ab16502, 1:2200; Abcam), and b-actin (ab8227, 1:1,200; Abcam). The membranes were next incubated with HRP-conjugated secondary antibody (goat anti-rabbit IgG, ab205718, 1:1800; Abcam) and the blots were visualized using ECL chemiluminescence reagent (Beyotime). b-actin was used as a normalized standard. ImageJ software (NIH, version 1.8.0) was employed for the semiquantitative analysis of protein expression.
Wu X., Zhang X., Zhao L, & Jiang S. (2022). Neuroprotective effect of AGGF1 against isoflurane-induced cognitive dysfunction in aged rats through activating the PI3K/AKT signaling pathways. Physiology international.
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