Primary antibodies were purchased from the respective vendors: rabbit anti-BCL9L (A303-152A; Bethyl), rabbit anti-IPO11 (A304-811A; Bethyl), rabbit ERK1/2 (#9102; Cell Signaling), rabbit βcatenin for Western blot (#9587; Cell Signaling), rabbit Lamin B1 (#16048; Abcam), mouse anti–β-tubulin clone TUB 2.1, rabbit anti-Flag (2368; Sigma-Aldrich), mouse anti-HA (clone 16B12; Covance), mouse myc antibody (#9E10; Abcam), and GST antibody (B-326, MA4-004). Secondary antibodies were conjugated to horseradish peroxidase (Jackson ImmunoResearch Laboratories Inc).
Mouse anti β tubulin clone tub 2
Manufactured by Merck Group
Mouse anti–β-tubulin clone TUB 2.1 is a monoclonal antibody that specifically binds to the β-tubulin subunit of microtubules. It can be used for the detection and localization of β-tubulin in various applications.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit erk1 2, by Cell Signaling Technology (1 mentions)
Rabbit lamin b1, by Abcam (1 mentions)
Rabbit anti flag, by Merck Group (1 mentions)
Mouse anti ha clone 16b12, by Fortrea (1 mentions)
Ob cadherin, by Cell Signaling Technology (1 mentions)
Goat anti rabbit, by Agilent Technologies (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
Goat anti mouse, by Agilent Technologies (1 mentions)
P cadherin, by Cell Signaling Technology (1 mentions)
Mouse anti syndecan 1 clone b a38, by Abcam (1 mentions)
Keratin 10, by Fortrea (1 mentions)
Mouse anti ha 11 clone 16b12, by Fortrea (1 mentions)
N cadherin, by Abcam (1 mentions)
2 protocols using mouse anti β tubulin clone tub 2
1
Antibody Identification and Validation
2
Immunofluorescence Antibody Protocol
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Monoclonal antibodies used in this study included rabbit anti-αSMA antibody (clone E184; Abcam), mouse anti–α-actinin (clone BM75.2; Sigma-Aldrich), mouse anti-HA.11 (clone 16B12; Covance), mouse anti–β-tubulin (clone TUB 2.1; Sigma-Aldrich), mouse anti-paxillin (clone 5H11; EMD Millipore), mouse anti–syndecan-1 (clone B-A38; Abcam), rat anti–syndecan-4 (clone KY/8.2; BD), mouse anti-p120 catenin (clone 6H11; Invitrogen), rat anti–E-cadherin (clone DECMA-1; Abcam), and rabbit anti-NFAT3 (clone 23E6; Cell Signaling Technology). Polyclonal antibodies used were rabbit antibodies against syndecan-4 (Abcam and LSBio), OB-cadherin (Cell Signaling Technology), N-cadherin (Abcam), P-cadherin (Cell Signaling Technology), keratin-10 (Covance), TRPC4 (Sigma-Aldrich), and TRPC7 (Sigma-Aldrich and EMD Millipore). Secondary antibodies included goat anti–rabbit, goat anti–mouse, and rabbit anti–rat IgG HRP-conjugated antibodies from Dako. Donkey and goat anti–mouse, goat anti–rabbit, and donkey and goat anti–rat IgG conjugated to Alexa Fluor 488, 568, or 647 were obtained from Molecular Probes. DAPI and Alexa Fluor 568– and 647–conjugated phalloidin were obtained from Molecular Probes.
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