Cells were transfected with sh-NC, sh-MALAT1, and a MALAT1 vector, which were purchased from GenePharma (Shanghai, China), to modulate the expression of MALAT1 in the absence or presence of LPS. The sequence of sh-MALAT1 was 5′-AAGGATGTCAGCGCACTAAAT-3′. Lipofectamine 3000 (Invitrogen, USA) was used for cell transfection according to the manufacturer’s protocols. ML385 and GSK-126 were used to inhibit Nrf2 or EZH2, respectively. The effect of MALAT1-transfected microglia on neurons was investigated using a Transwell co-culture system. Briefly, an N2a neuronal cell was added to the lower chamber of the 96-well plate, and the BV2 cells with different treatments were cultured on the Transwell inserts (pore size 0.4 μm; Corning, USA) in the 96-well plate.
Sh malat1
Manufactured by Thermo Fisher Scientific
Sourced in United States
Sh-MALAT1 is a laboratory equipment product offered by Thermo Fisher Scientific. It is designed to detect and quantify the expression of the MALAT1 gene, which is a long non-coding RNA involved in various cellular processes. The core function of Sh-MALAT1 is to provide researchers with a tool to study the expression and regulation of the MALAT1 gene in their experiments.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions)
Sh malat1, by GenePharma (1 mentions)
Malat1 vector, by GenePharma (1 mentions)
Sh nc, by GenePharma (1 mentions)
Transwell insert, by Corning (1 mentions)
Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions)
Inhibitor nc, by Thermo Fisher Scientific (1 mentions)
Mir 206 inhibitor, by GenePharma (1 mentions)
Sh nc, by Thermo Fisher Scientific (1 mentions)
Mir 206 inhibitor, by Thermo Fisher Scientific (1 mentions)
Mir 206 mimic, by GenePharma (1 mentions)
Mir nc, by Thermo Fisher Scientific (1 mentions)
Inhibitor nc, by GenePharma (1 mentions)
Mir nc, by GenePharma (1 mentions)
3 protocols using sh malat1
1
Modulating MALAT1 Expression in Microglia
2
Overexpression and Knockdown of Key Regulators
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ZNF217 cDNA was synthesized and cloned into pcDNA3.1 plasmid for overexpression and pcDNA3.1 plasmid contains a scrambled sequence was used as negative control (NC). Sh-MALAT1, sh-NC, pcDNA3.1, pcDNA-ZNF217, miR-141-3p mimics (sense 5′-UAACACUGUCUGGUAAAGAUGG-3′ and antisense 5′-AUCUUUACCAGACAGUGUUAUU-3′), mimics NC (5′- UUCUCCGAACGUGUCACGUTT-3′), miR-141-3p inhibitor (5′-CCAUCUUUACCAGACAGUGUUA-3′) and inhibitor NC (5′-CAGUACUUUUGUGUAGUACAA-3′) were all constructed or synthesized by GenePharma (Shanghai, China). The pGMLV-SC5 RNAi lentivirus plasmid (GeneChem) was used to knock down MALAT1 expression in mice model. In brief, HFF1 cells were plated overnight to reach 50% confluence before transfection. Sh-MALAT1, miR-141-3p mimics, miR-141-3p inhibitor, pcDNA-ZNF217 and their controls were transfected into HFF-1 cells with lipofectamine 2000 reagent (Invitrogen, Carlsbad, USA) according to the manufacturer's instructions.
3
Regulation of MALAT1, miR-206, ARNT, and PPARα in HepG2 cells
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The shRNA MALAT1 (sh-MALAT1), sh-negative control (sh-NC), miR-206 inhibitor, inhibitor NC, miR-206 mimics, miR-NC, sh-ARNT and sh-PPARα were obtained from GenePharma (Shanghai, China). HepG2 cells transfected with sh-MALAT1, sh-NC, miR-206 inhibitor, inhibitor NC, miR-206 mimics, miR-NC, sh-ARNT or sh-PPARα by using Lipofectamine 3000 (Invitrogen).
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