Nuclei were stained with Hoechst33342 (Invitrogen). Cytochrome c was measured using the Cytochrome c-Alexa647 antibody (BioLegend). Mouse tumor cells were identified with Mouse EpCam-FITC (BioLegend). Colon tumor cells were identified using human EpCam-Alexa488 (Biolegend)
Cytochrome c alexa647 antibody
Manufactured by BioLegend
Cytochrome c-Alexa647 antibody is a fluorochrome-conjugated antibody that recognizes cytochrome c, a protein involved in the electron transport chain during cellular respiration. The Alexa Fluor 647 dye is covalently attached to the antibody, allowing for detection and quantification of cytochrome c expression using flow cytometry or other fluorescence-based applications.
Automatically generated - may contain errors
Lab products found in correlation
Hoechst 33342, by Thermo Fisher Scientific (3 mentions)
Vimentin, by BioLegend (1 mentions)
Phospho akt ser473, by Cell Signaling Technology (1 mentions)
Bcl xl, by Cell Signaling Technology (1 mentions)
Mcl 1, by Cell Signaling Technology (1 mentions)
Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions)
Bcl 2, by Cell Signaling Technology (1 mentions)
Cleaved caspase 3, by Cell Signaling Technology (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
3 protocols using cytochrome c alexa647 antibody
1
Multicolor Staining of Tumor Cells
2
Multiparametric Cell Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Nuclei were stained with Hoechst33342 (1:2000; Life Technologies) to determine a total number of cells. Cytochrome c positive cells % was measured using cytochrome c-Alexa647 antibody (#612310; 1:2000; BioLegend). A pan cytokeratin antibody (#628608; 1:1000; Biolegend) was used to identify epithelial tumor cells parent population %. BIM (C34C5) #2933S (1:1000) and Beta-Actin #4967S (1:2000) were used for Western blotting (Cell Signaling Technology).
3
Multiplex Immunofluorescence Analysis of MPM
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For DBP and HTDBP, nuclei were stained with Hoechst 33342 (1:2000; Life Technologies) to determine total number of cells. A pan-cytokeratin antibody (#628608; 1:1000; Biolegend) was used to identify epithelioid MPM tumor cells parent population and vimentin (#677809; 1:1000; Biolegend) was used to identify sarcomatoid MPM parent population. Cytochrome c positive cells % was measured using cytochrome c-Alexa647 antibody (#612310; 1:2000; Biolegend). Western blotting was performed per manufacturer specifications (BIO-RAD). For Western blotting all antibodies were from Cell signaling Technology unless otherwise stated, β-Actin (#4967S 1:2000), AKT (#4685S 1:1000), phosphoSer473-AKT (#4685S 1:1000), BIM (#2933S 1:1000), BAK (#12105S 1;1000), BAX (#2772S 1;1000), BCL-xL (#2764S 1;1000), BCL-2 (#15071S 1;1000), cleaved Caspase 3 (#9661S 1:1000), MCL-1 (#39224S 1;1000; human specific for PDX samples), MCL-1 (#94296S 1;1000), PUMA (#4976S 1;1000), PARP (#9542S 1;1000), S6 (#2317S 1;1000) and phosphoSer235/236-S6 (#4857S 1;1000).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!