Alt and ast assay kit

The toxicity to the liver, kidneys, heart, and testis was examined by H&E staining. Furthermore, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were measured with the ALT and AST Assay Kit (Nanjing Jiancheng Bioengineering, Nanjing, China). The creatinine (Cr), lactate dehydrogenase (LDH), and creatine kinase MB (CK-MB) levels were measured with a Hitachi automatic biochemical analyzer 7600 (Shiga, Japan).

The levels of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured utilizing ALT and AST assay kits (Nanjing Jiancheng, Nanjing, China) to evaluate liver function and liver injury.

Whole blood was harvested and serum was prepared as previously described [30 (link)]. Liver tissues of septic mice were homogenized with PBS (10 mg tissues/100 μl of PBS), and the supernatants of homogenates were collected according to the manufacturer's instructions. Serum alanine transaminase (ALT) and aspartate transaminase (AST) levels were measured using commercial ALT and AST assay kits (Jiancheng, Nanjing, China) to reflect liver injury. Increased serum levels of inflammatory cytokines are critical pathological factors and valuable indicators of sepsis [31 (link)]. The levels of inflammatory factors including IL-6, IL-10, IL-1β, and TNF-α in serum and liver homogenates were determined with ELISA kits (eBioscience, USA) to indicate systemic inflammation.

1) Hepatic enzymatic changes: Serum alanine aminotransferase (ALT) and serum aspartate aminotransferase (AST) were measured using ALT and AST assay kits (Lot number 20180628, Nanjing Jiancheng Bioengineering Institute, Nanjing, China). 2) Glucose metabolism: Following 12 h of fasting, approximately 3 μL of blood was collected from the tail vein, and fasting blood glucose (FBG) was measured using a Roche blood glucose meter (Roche diagnostic GmbH, Germany). Fasting insulin (FINS) levels in the serum of mice from each group were measured using an enzyme-linked immunosorbent assay (ELISA) kit (Ultra Sensitive Mouse Insulin ELISA Kit, lot number: 90080, Crystal Chem, USA). The homeostatic model assessment-insulin resistance (HOMA-IR) index was calculated with the following formula: FBG (mM) x FIN (IU/L)/22.5. 4) Liver triglyceride (TG) content was measured using a kit (Lot number: 2018080029, Dong’ou Diagnostic Products Co. Ltd., Zhejiang, China). 5) Liver hydroxyproline (HYP) content was measured using an HYP assay kit (lot number: 20180630, Nanjing Jiancheng Bioengineering Institute, Nanjing, China).

Serum samples were obtained from blood after 30 min centrifugation at 4 °C. The levels of Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were assessed by ALT and AST assay kits (Nanjing Jiancheng, Nanjing, China).

Serum samples were collected and analyzed for alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels with ALT and AST assay kits according to the manufacturer’s instructions (Nanjing Jiancheng).

To determine the MPO levels, 10% tissue homogenates were prepared using MPO buffer and detected by MPO assay kit according to the manufacturer’s instructions (Nanjing Jiancheng Bioengineering Institute, Nanjing, China). To investigate liver injury, the serum ALT and AST were determined using ALT and AST assay kits according to the manufacturer’s instructions (Nanjing Jiancheng Bioengineering Institute, Nanjing, China).