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Il 25

Manufactured by Qiagen
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IL-25 is a lab equipment product manufactured by Qiagen. It is a protein that functions as a cytokine, playing a role in immune system regulation. The core function of IL-25 is to stimulate the production of other cytokines and promote inflammatory responses. Further details on its intended use are not available.

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Lab products found in correlation

Il 13, by Qiagen (2 mentions) Il 33, by Qiagen (2 mentions) Interleukin 4 (il 4), by Qiagen (2 mentions) Il 10, by Qiagen (2 mentions) Sybr green chemistry, by Thermo Fisher Scientific (2 mentions) Abi 7500 fast real time pcr system, by Thermo Fisher Scientific (2 mentions) Il 17a, by Qiagen (2 mentions) Quantitech primer assay, by Qiagen (2 mentions) Trizol, by Thermo Fisher Scientific (2 mentions) Superscript reverse transcriptase, by Thermo Fisher Scientific (2 mentions)

2 protocols using il 25

1

Quantitative RT-PCR Analysis of Lung Cytokines

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA from sections of lung tissue was isolated by homogenization in TRIzol (Invitrogen) followed by phenol-chloroform extraction and isopropanol precipitation. cDNA was generated per standard protocol with Superscript reverse transcriptase (Invitrogen) and used as input for real-time PCR. Real-time PCR data were analyzed using the ΔΔCT method using SYBR Green chemistry (Applied Biosystems), with βNA fro serving as the endogenous housekeeping gene. All reactions were run on an ABI 7500 Fast Real-Time PCR System (Applied Biosystems). Samples were normalized to naïve controls. The following QuantiTech primer assays from Qiagen were used: Areg (QT00112217), IL-4 (QT00160678), IL-5 (QT00099715), IL-10 (QT00106169), IL-13 (QT00099554), IL-17a (QT00103278), IL-25 (QT00134645), IL-33 (QT00135170), Mcpt8 (QT00131565), Muc5ac (QT01161104), Nmb (QT00105945), Nmbr1 (QT00312494), Nmu (QT00133091), Nmur1 (QT00174006), and Tslp (QT00198261).
Inclan-Rico J.M., Ponessa J.J., Valero-Pacheco N., Hernandez C.M., Sy C.B., Lemenze A.D., Beaulieu A.M, & Siracusa M.C. (2020). Basophils prime group 2 innate lymphoid cells for neuropeptide-mediated inhibition. Nature immunology, 21(10), 1181-1193.
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2

Quantitative RT-PCR Analysis of Lung Cytokines

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA from sections of lung tissue was isolated by homogenization in TRIzol (Invitrogen) followed by phenol-chloroform extraction and isopropanol precipitation. cDNA was generated per standard protocol with Superscript reverse transcriptase (Invitrogen) and used as input for real-time PCR. Real-time PCR data were analyzed using the ΔΔCT method using SYBR Green chemistry (Applied Biosystems), with βNA fro serving as the endogenous housekeeping gene. All reactions were run on an ABI 7500 Fast Real-Time PCR System (Applied Biosystems). Samples were normalized to naïve controls. The following QuantiTech primer assays from Qiagen were used: Areg (QT00112217), IL-4 (QT00160678), IL-5 (QT00099715), IL-10 (QT00106169), IL-13 (QT00099554), IL-17a (QT00103278), IL-25 (QT00134645), IL-33 (QT00135170), Mcpt8 (QT00131565), Muc5ac (QT01161104), Nmb (QT00105945), Nmbr1 (QT00312494), Nmu (QT00133091), Nmur1 (QT00174006), and Tslp (QT00198261).
Inclan-Rico J.M., Ponessa J.J., Valero-Pacheco N., Hernandez C.M., Sy C.B., Lemenze A.D., Beaulieu A.M, & Siracusa M.C. (2020). Basophils prime group 2 innate lymphoid cells for neuropeptide-mediated inhibition. Nature immunology, 21(10), 1181-1193.
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