Corn oil

After radiation, p65 shifts between the cytoplasm and the nucleus were used to evaluate the activation of NF-κB. 4-Methyl-N1-(3-phenyl-propyl)-benzene-1,2-diamine (JSH-23) (MedChemExpress, Monmouth Junction NJ, USA), an inhibitor of NF-κB, which inhibits nuclear translocation of p65 without affecting IκBα degradation, can inhibit NF-κB transcriptional activity with an IC50 of 7.1 μM in lipopolysaccharide (LPS)-stimulated macrophages RAW 264.7 and is used to inhibit NF-κB activation in this study.
For one week before radiation and one week after radiation, JSH-23 was administered every other day via intraperitoneal injection for a total of seven doses. The detailed administration method was as follows: on the day of administration, 10 mg JSH-23 was dissolved in 3.6 mL corn oil (MedChemExpress) mixed with 0.4 mL DMSO (MedChemExpress), and then diluted 10 times with isotonic saline and administered to the mice via intraperitoneal injection at a dose of 5 mg/kg according to the manufacturer’s protocol.

8-week-old C57BL/6 female mice (n = 15) weighing 20 to 25 g were anesthetized with 3 mg/mL pentobarbital for bilateral ovariectomy (OVX). The mice were randomly divided into three groups (each; n = 5). The sham and vehicle group were injected with corn oil (MedChemExpress, NJ, USA) containing 10% DMSO (MedChemExpress), and the SR1 group was injected with SR1 (2.5 mg/kg) every 3 days for 42 days. Next, the isolated femur and tibia were fixed in 4% paraformaldehyde for 48 h and the micro-CT and histological experiments were carried out. The study protocol was approved by the Research Ethics Committee of the First Affiliated Hospital of Zhejiang University. All efforts were made to minimize the number and suffering of animals.