Immortal human keratinocytes HaCaT were accessed from Cell Resource Center, Institute of Basic Medicine, Chinese Academy of Medical Sciences. FaDu (BNCC316798), SCC-9 (BNCC250260), SCC-15 (BNCC275715), and SCC-25 (BNCC315876) were all human HNSCC cell lines and were bought from BeNa Culture Collection (BNCC). They were maintained in DMEM with 10% fetal bovine serum (FBS) and hydrocortisone (400 ng/mL). HaCaT cells were incubated in MEM-EBSS with 15% FBS. FaDu cells were maintained in Eagle's MEM (No. 30-2003) (American Type Culture Collection (ATCC)) with 10% FBS. Culture condition was an incubator with 5% CO2 at 37°C. After infusion degree of cells reached 80%, they were used for subculture or assays.
Scc 9
Manufactured by BNCC
Sourced in China
The SCC-9 is a laboratory centrifuge designed for general-purpose applications. It features a compact and durable construction, providing reliable performance for routine sample separation and preparation tasks. The SCC-9 is capable of accommodating a variety of sample tubes and volumes, making it a versatile tool for various laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Scc25, by BNCC (4 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (3 mentions)
Scc 15, by BNCC (2 mentions)
Cal27, by BNCC (2 mentions)
Hsc 3, by BNCC (2 mentions)
Scc 9, by GenePharma (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Tca8113, by BNCC (1 mentions)
Scc 4, by BNCC (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Defined keratinocyte serum free medium, by Thermo Fisher Scientific (1 mentions)
Hsc 3, by Thermo Fisher Scientific (1 mentions)
Scc25, by Thermo Fisher Scientific (1 mentions)
Cal27, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Vero ccl 81 cells, by American Type Culture Collection (1 mentions)
Cycloheximide hy 12320, by MedChemExpress (1 mentions)
6 protocols using scc 9
1
Optimized Culture of HNSCC Cell Lines
2
Transfection of Human Oral Carcinoma Cells
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Human oral keratinocytes (HOK-16B), human tongue squamous cell carcinoma (CAL-27, HSC-4 and SCC-9) cells were purchased from BeNa Culture Collection (Beijing Beina Chunglian Institute of Biotechnology) and were cultured in DMEM (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS (Gibco; Thermo Fisher Scientific, Inc.) at 37°C in a humidified atmosphere with 5% CO2.
The pcDNA3.1-cyclin A2 (CCNA2) and empty pcDNA3.1 vectors, short hairpin RNA (shRNA) targeting PKMYT1 (shRNA-PKMYT1-1, target sequence: 5′-CTATGCGGTAAAGCGTTCCAT−3′ and shRNA-PKMYT1-2, target sequence: 5′-GCTGCGTTCTGTCCTTGTCAT−3′) and a nonspecific sequence used as a negative control (NC; target sequence: 5′-CAACAAGATGAAGAGCACCAA−3′) were purchased from Shanghai GenePharma Co., Ltd. SCC-9 cells (5×105 cells/well) were seeded into 6-well plates and transfected with 10 nM pcDNA plasmid or shRNA using Lipofectamine® 3000 (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. Following 6 h of transfection, the transfection reagent in the wells were replaced with fresh DMEM supplemented with 10% FBS and cells were cultured for 2 days (all from Gibco; Thermo Fisher Scientific, Inc.).
The pcDNA3.1-cyclin A2 (CCNA2) and empty pcDNA3.1 vectors, short hairpin RNA (shRNA) targeting PKMYT1 (shRNA-PKMYT1-1, target sequence: 5′-CTATGCGGTAAAGCGTTCCAT−3′ and shRNA-PKMYT1-2, target sequence: 5′-GCTGCGTTCTGTCCTTGTCAT−3′) and a nonspecific sequence used as a negative control (NC; target sequence: 5′-CAACAAGATGAAGAGCACCAA−3′) were purchased from Shanghai GenePharma Co., Ltd. SCC-9 cells (5×105 cells/well) were seeded into 6-well plates and transfected with 10 nM pcDNA plasmid or shRNA using Lipofectamine® 3000 (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. Following 6 h of transfection, the transfection reagent in the wells were replaced with fresh DMEM supplemented with 10% FBS and cells were cultured for 2 days (all from Gibco; Thermo Fisher Scientific, Inc.).
3
Isolation and Culture of Oral Keratinocytes
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Primary normal human oral keratinocytes (NHOKs) were isolated from keratinized oral epithelial tissues of patients who suffered from flap operation to remove impacted wisdom teeth. This experiment was carried out with the written informed consents from the patients and the approval of Ethics Committee of Changhai Hospital. NHOKs were cultured in Defined Keratinocyte-serum-free medium (Thermo Fisher Scientific, Waltham, MA, USA). Human TSCC cell lines (Tca8113, SCC-2, SCC-4, SCC-9, and Cal-27) were purchased from BeNa Culture Collection (Beijing, China) and incubated in Roswell Park Memorial Institute (RPMI) 1640 medium (Thermo Fisher Scientific) supplemented with 10% FBS (Thermo Fisher Scientific). All cells were grown in a humidified incubator containing 5% CO2 at 37°C.
4
Culturing OSCC Cell Lines
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OSCC cell lines, including Cal-27, SCC-25, SCC9 and HSC-3, and human oral keratinocytes (HOK) were purchased from BeNa Culture Collection (Beijing, China). Cal-27 cells and HSC-3 cells were cultured in 90% DMEM (GIBCO, Grand Island, NY, USA) containing 10% FBS (GIBCO). SCC-25 cells were cultured in 90% EMEM (GIBCO) containing 10% FBS. SCC9 cells and HOK cells were cultured in 90% RPMI1640 (GIBCO) containing 10% FBS. These cells were maintained in a 37 incubator with 5% CO2.
5
Culturing OSCC and NHOK Cells
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OSCC cell lines (HSC3, SCC9, SCC15 and SCC25) and primary normal human oral keratinocyte (NHOK) cells were purchased from the BeNa Culture Collection (BNCC, Shanghai, China). RPMI-1640 medium containing 10% FBS (Gibco; Thermo Fisher Scientific, Inc.), 100 U/ml penicillin and 100 µg/ml streptomycin were used to culture OSCC cells and then cells were incubated at 37°C with 5% CO2 atmosphere.
6
Investigating Oncolytic HSV-1 Efficacy in OSCC Cell Lines
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OSCC lines SCC9 and SCC25 were obtained from the BeNa Culture Collection (BNCC, China), SCC7 was gifted from Prof. Bernard Roizman at the University of Chicago. All these cells were cultured in DMEM (Thermo Scientific, USA) supplemented with 10% fetal bovine serum (FBS; Thermo Scientific, USA). Vero (CCL-81) cells were purchased from the American Type Culture Collection (USA) and cultured in DMEM supplemented with 50% newborn calf serum (NBCS; Thermo Scientific, USA). All cell lines were incubated at 37°C in a humidified atmosphere of 5% CO2. Oncolytic HSV-1 recombinant virus T1012G (oHSV-1 T1012G) was previously described.14 Immunoglobulin (Ig)G (S20043008) was obtained from the Shenzhen Weiguang Biological Co., Ltd, China. Cycloheximide (HY-12320) and GSK2643943A (HY-111458) were purchased from MedChem Express (MCE, USA).
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