Quickplex sq 120 apparatus

Manufactured by Mesoscale

Sourced in United States

The QuickPlex SQ 120 is a multiplex assay platform that enables the simultaneous detection and quantification of multiple analytes in a single sample. The apparatus utilizes electrochemiluminescence technology to provide accurate and sensitive measurements.

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Lab products found in correlation

Mouse 5 plex proinflammatory panel 1 kit, by Mesoscale (3 mentions) Mouse il 1ra elisa kit, by Thermo Fisher Scientific (2 mentions) Mouse leptin kit, by Mesoscale (2 mentions) Free glycerol reagent, by Merck Group (1 mentions) Triglyceride reagent, by Merck Group (1 mentions)

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QuickPlex SQ 120 (92 citations)

3 protocols using quickplex sq 120 apparatus

Cytokine Quantification from Plasma and Tissue

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For blood analysis, plasmas were diluted twice and analysis following manufacturer’s instructions using the mouse V-PLEX Proinflammatory Panel 1 Kit (Meso Scale Discovery, # K15048D) on a QuickPlex SQ 120 apparatus (Meso Scale Discovery).
For tissue analysis, proteins were extracted from frozen organs using an ULTRA TURRAX T25 (Ika, Germany) and lysis buffer (25 mM Tris-Cl (pH 7.4), 100 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.5% Nonidet P40 and protease inhibitors (Roche Diagnostics, Meylan, France)). Protein concentration was evaluated by BCA assay (Sigma-Aldrich Chimie, Saint-Quentin Fallavier, France). 10 µg proteins were used to evaluate cytokine concentration using the same kit and apparatus as those used for blood cytokine analysis.

Colson C., Ghandour R.A., Dufies O., Rekima S., Loubat A., Munro P., Boyer L, & Pisani D.F. (2019). Diet Supplementation in ω3 Polyunsaturated Fatty Acid Favors an Anti-Inflammatory Basal Environment in Mouse Adipose Tissue. Nutrients, 11(2), 438.

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Adipose Tissue Secretome Analysis

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For blood analysis, freshly prepared plasmas were diluted twice before analysis. For tissue analysis, freshly sampled WAT and BAT were washed in PBS, weighed and incubated in free DMEM (Dulbecco’s modified Eagle medium) for 2 hours at 37°C. Media were kept for various analyses of secreted proteins.
Leptin and cytokines were assayed using “Mouse Leptin Kit” (Meso Scale Discovery, # K152BYC) and “mouse V-PLEX Proinflammatory Panel 1 Kit” (Meso Scale Discovery, # K15048D) respectively, on a QuickPlex SQ 120 apparatus (Meso Scale Discovery) following manufacturer’s instructions. IL-1RA levels were assayed using mouse IL-1RA Elisa kit (#EMIL1RN) from Thermo Fisher Scientific (Courtaboeuf, France). Glycerol and triglycerides determinations were performed using dedicated kit (Free Glycerol reagent and Triglyceride reagent, Sigma Aldrich).

Munro P., Rekima S., Loubat A., Duranton C., Pisani D.F, & Boyer L. (2021). Impact of thermogenesis induced by chronic β3-adrenergic receptor agonist treatment on inflammatory and infectious response during bacteremia in mice. PLoS ONE, 16(8), e0256768.

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Adipose Tissue Secretome Analysis

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For blood analysis, freshly prepared plasma was diluted twice before analysis. For tissue analysis, freshly sampled WAT and BAT were washed in PBS, weighed and incubated in free Dulbecco's modified Eagle's medium for 2 hours at 37°C. The media were preserved for analysis of various secreted proteins.
Leptin was assayed using a mouse leptin kit (Meso Scale Discovery, # K152BYC), and cytokines were measured using a mouse V-PLEX pro-inflammatory panel 1 kit (# K15048D) according to the manufacturer's instructions using a QuickPlex SQ 120 apparatus (Meso Scale Discovery, Rockville, Maryland, USA). IL-1RA levels were assayed using a mouse IL-1RA
ELISA kit (#EMIL1RN) from Thermo Fisher Scientific (Courtaboeuf, France). Glycerol and triglyceride determinations were performed using a dedicated kit (free glycerol reagent and triglyceride reagent, Sigma Aldrich).

Munro P., Dufies O., Rekima S., Loubat A., Duranton C., Boyer L, & Pisani D.F. (2020). Modulation of the inflammatory response to LPS by the recruitment and activation of brown and brite adipocytes in mice. American journal of physiology. Endocrinology and metabolism, 319(5).

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