The medium was partially modified by Clostridium cellulovorans medium (Sleat et al. 1984 (link)). One litter medium containing 4 g of yeast extract, 1 mg of Resazurin salt, 1 g of l -cysteine-HCl, 5 g of NaHCO3, 0.45 g of K2HPO4, 0.45 g of KH2PO4, 0.3675 g of NH4Cl, 0.9 g of NaCl, 0.1575 g of MgCl2∙6H2O, 0.12 g of CaCl2∙2H2O, 0.85 mg of MnCl2∙4H2O, 0.942 mg of CoCl2∙6H2O, 5.2 mg of Na2EDTA, 1.5 mg of FeCl2∙4H2O, 0.07 mg of ZnCl2, 0.1 mg H3BO3, 0.017 mg of CuCl2∙2H2O, 0.024 mg of NiCl2∙6H2O, 0.036 mg of Na2MoO4∙2H2O, 6.6 mg of FeSO4∙7H2O, and 0.1 g of p-aminobenzoic acid and was adjusted to pH7. C. cellulovorans 743B (ATCC 35296) was used and anaerobically cultivated in 0.5% (w/v) cellobiose (Sigma, MO, USA) at 37 °C for 19 h stationary. The MFMP was obtained from methane fermentation digested liquid on January, 2017 at Gifu in Japan. The MFMP was anaerobically cultivated in Clostridium cellulovorans medium with 0.5% (w/v) glucose (Wako) and 0.25% (w/v) cellobiose at 37 °C for 19 h stationary.
Cellobiose
Manufactured by Fujifilm
Sourced in Japan
Cellobiose is a disaccharide composed of two glucose molecules linked by a β-1,4 glycosidic bond. It is a common byproduct of cellulose hydrolysis and serves as a substrate for cellulase enzymes. Cellobiose can be used in various lab applications, such as enzyme activity assays and cellulose degradation studies.
Automatically generated - may contain errors
Lab products found in correlation
Cellobiose, by Merck Group (1 mentions)
Galactose, by Fujifilm (1 mentions)
Sucrose, by Fujifilm (1 mentions)
Lactulose, by Fujifilm (1 mentions)
Maltose monohydrate, by Fujifilm (1 mentions)
Mannose, by Fujifilm (1 mentions)
Raffinose pentahydrate, by Fujifilm (1 mentions)
Melezitose, by Merck Group (1 mentions)
Trehalose dihydrate, by Fujifilm (1 mentions)
Lactose monohydrate, by Fujifilm (1 mentions)
Laminaribiose, by Merck Group (1 mentions)
Cellotetraose, by Merck Group (1 mentions)
Sophorose, by Merck Group (1 mentions)
Cellopentaose, by Merck Group (1 mentions)
Gentiobiose, by Merck Group (1 mentions)
Cellotriose, by Merck Group (1 mentions)
Laminaritriose, by Merck Group (1 mentions)
Laminaritetraose, by Merck Group (1 mentions)
Fructose, by Fujifilm (1 mentions)
Levoglucosan, by Fujifilm (1 mentions)
5 protocols using cellobiose
1
Anaerobic Cultivation of C. cellulovorans and MFMP
2
Recombinant Cellobiose Production Protocol
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α-G1P and pET-28b vector were purchased from Sigma-Aldrich Co. LLC (MO, US). Cellobiose and other chemical reagents were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Overnight Express auto-induction medium and BugBuster reagents were purchased from Merck KgaA (Darmstadt, Germany). CDP from Clostridium (Hungateiclostridium) thermocellum strain YM4 was initially provided by Prof. Momomitsu Kitaoka of Niigata University, Japan. E. coli BL21 (DE3) competent cells were purchased from Nippon Gene (Tokyo, Japan). C-Tube-LC counter-diffusion (Otálora et al. 2009 (link)) quartz capillaries were purchased from Confocal Science Inc. (Tokyo, Japan).
3
Synthesis of Cholesterol-Based Lipid Conjugates
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Cholesteryl (3-((2-hydroxyethyl)amino)propyl)carbamate hydroiodide (HAPC-Chol) was synthesized as described previously (13 (link)). N-(2-(2-Hydroxyethylamino)ethyl)cholesteryl- 3-carboxamide (OH-Chol) and cholesteryl (2-((2-hydroxyethyl)amino)ethyl)carbamate (OH-C-Chol) were synthesized as described previously (14 (link)). 1,2-Dioleoyl- 3- trimethylammonium-propane methyl sulfate salt (DOTAP) was obtained from Avanti Polar Lipids Inc. Dimethyldioctadecylammonium bromide (DDAB, product name: DC-1-18) and 11- ((1,3- bis(dodecanoyloxy)- 2- ((dodecanoyloxy)methyl)propan- 2- yl) amino)- N,N,N- trimethyl-11- oxoundecan-1- aminium bromide (product name: TC-1-12) were obtained from Sogo Pharmaceutical Co., Ltd.. 1,2-Dioleoyl- sn- glycero- 3-phosphoethanolamine (DOPE, COATSOME ME-8181) was obtained from NOF Co., Ltd.. Glucose, fructose, galactose, mannose, sucrose, trehalose dihydrate, maltose monohydrate, lactose monohydrate, lactulose, cellobiose, and raffinose pentahydrate were obtained from Wako Pure Chemical Industries, Ltd.. Melezitose was purchased form Sigma-Aldrich Co. LLC. All other chemicals were of the highest grade available.
4
HPAEC-PAD for Enzymatic Reaction Products
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For detection of enzymatic reaction products, high-performance anion exchange column chromatography (HPAEC) with a pulsed amperometoric detector (PAD) equipped with a CarboPac PA10 guard column (4 × 50 mm) and a CarboPac PA10 analytical column (4 × 250 mm; Dionex Co.) was used. Enzymatic reaction was performed by incubation with equivalent volume of rAaBGL1 (20.0 nM) and each substrate in 20 mM sodium acetate buffer (pH 5.0) at 37°C. Reaction mixture was sampled at appropriate time, and added into equal volume of 0.2 M NaOH. Resultant mixtures were subjected to HPAEC-PAD using mobile phase of 100 mM NaOH with 10 mM sodium acetate. Glucose, cellobiose (Wako Pure Chemical Industries, Ltd.), cellotriose, cellotetraose, cellopentaose, laminaribiose, laminaritriose, laminaritetraose, laminaripentase (Megazyme), gentiobiose, sophorose (SIGMA-ALDRICH, Co.) were used as standards.
5
Characterization of Cellulose and Wood Flour
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Table 1 lists the ionic liquids that were used in this study. 5-HMF, glucose, fructose, cellobiose, cellotriose, levoglucosan, 1,6-anhydro-b-D-glucofuranose (AGF), acetonitrile, sodium sulfate, and acetic acid were purchased from Wako Pure Chemical Industries (Osaka, Japan). N,O-Bis(trimethylsilyl)trifluoroacetamide (BSTFA) and dehydrated dimethyl sulfoxide were purchased from Sigma-Aldrich (St Louis, MO, USA). Sodium chlorite and pyridine were purchased from Kanto Chemicals (Tokyo, Japan). Cellulose sample (Whatman CF1) was dried in an oven at 105 °C for 24 h before use. Wood flours from Japanese cedar (C. japonica) (90-180 lm) were extracted with ethanol/benzene (1/2, v/v) for 24 h in a Soxhlet apparatus. The extractive-free wood flour was oven-dried at 105 °C for 24 h before use.
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