The serum HMGB1, HMGB2, interleukin (IL)-6, IL-1β, interferon-γ (IFN-γ), and C-reactive protein (CRP) levels were measured by enzyme-linked immunosorbent assay (ELISA). Blood samples of fasting cubital venous (5 mL) were collected within 24 hours after admission for all cases. Samples were centrifuged at 2000 g for 15 minutes, following with ELISA tested using commercially available kits (HMGB1 MBS3803280 MyBioSource, HMGB2 MBS763599 MyBioSource, IL-6 MBS175877 MyBioSource, CRP MBS177184 MyBioSource, IL-1β MBS175901 MyBioSource, IFN-γ EK0373 BOSTER).
Il 1β mbs175901
Manufactured by MyBioSource
IL-1β (Interleukin-1 Beta) ELISA Kit MBS175901 is a quantitative sandwich enzyme-linked immunosorbent assay designed for the measurement of IL-1β levels in human, mouse, and rat samples. The kit utilizes a monoclonal antibody specific for IL-1β coated on a 96-well plate. Samples and standards are pipetted into the wells, and any IL-1β present is bound by the immobilized antibody. After washing, an enzyme-linked polyclonal antibody specific for IL-1β is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added and color develops in proportion to the amount of IL-1β bound. The intensity of the color is measured at a specific wavelength.
Automatically generated - may contain errors
3 protocols using il 1β mbs175901
1
Serum Biomarker Levels in Patients
2
Inflammatory Biomarkers in Clinical Assessment
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The serum ACSL4, tumor necrosis factor-alpha (TNF-α), interleukin (IL)-6, IL-1β and C-reactive protein (CRP) levels were measured by enzyme-linked immunosorbent assay (ELISA). Blood samples of fasting cubital venous (5 mL) were collected within 24 hours after admission for all cases. Samples were centrifuged at 2000 g for 15 minutes, following with ELISA tested using commercially available kits (ACSL4 MBS9331516 MyBioSource, TNF-α MBS824943 MyBioSource, IL-6 MBS175877 MyBioSource, CRP MBS177184 MyBioSource, IL-1β MBS175901 MyBioSource).
3
Inflammatory Biomarkers in Hospitalized Patients
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The serum NLRP1, tumor necrosis factor α (TNF-α), interleukin (IL)-6, IL-1β and C-reactive protein (CRP) levels were measured by enzyme-linked immunosorbent assay (ELISA). Blood samples of fasting cubital venous (5 mL) were collected within 24 hours after admission for all cases. Samples were centrifuged at 2000 g for 15 minutes, followed with ELISA tested using commercially available kits (NLRP1 MBS924094 MyBioSource, IL-6 MBS175877 MyBioSource, CRP MBS177184 MyBioSource, TNF-α MBS824943 MyBioSource, IL-1β MBS175901 MyBioSource). In addition, routing whole blood test was performed using an automatic biochemical analyzer (Hitachi 7600, Hitachi Corporation, Japan) and the levels of interferon-γ and IL-4 were recorded. All these inflammatory factors were measured when the patients were just hospitalized.
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