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Jkc 363

Manufactured by Bio-Techne
Sourced in United States

The JKC-363 is a laboratory instrument designed for precise sample processing and analysis. It is a compact and versatile device that can perform a range of tasks, including sample preparation, reagent handling, and data acquisition. The JKC-363 is equipped with advanced sensors and control systems to ensure accurate and reliable results.

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2 protocols using jkc 363

1

Kinase Pathway Activation Analysis

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[Nle(4), D-Phe(7)]melanocyte-stimulating hormone (NDP-MSH), JKC-363 and SB-747651A were purchased from Tocris Bioscience. PD98059 was purchased from Selleck Chemicals. Antibodies against phosphorylated ERK, MSK1 (Thr581), CREB (Ser133), histone H3 and phosphorylated histone H3 (Ser10) were obtained from Cell Signaling Technology. Antibodies specific for total ERK, MSK1 and CREB were purchased from Santa Cruz Biotechnology. pDC316 plasmids encoding EGFP or C-terminal kinase-dead (CKD) MSK1 were kind gifts of Dr. Tao Li (Department of Biology, College of Chemistry and Life Sciences, Zhejiang Normal University).
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2

Melanocortin Receptor Agonist Protocol

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[Nle 4 ,D-Phe 7 ]-α-melanocyte-stimulating hormone (NDP-MSH) was purchased from Bachem (CA, USA). Sodium palmitate was purchased from Santa Cruz Biotechnology (TX, USA).
Bovine serum albumin, Fraction V, fatty acid free was obtained from Roche (Grenzach, Germany). JKC363 was purchased from Tocris Bioscience (MO, United States). Fetal bovine serum (FBS) was obtained from Natocor (Cordoba, Argentina). Nrf2 antibody (Novus) was kindly provided by Dr. Cymeryng (CEFYBO, UBA-CONICET). DMEM/F-12, DMEM, L-Glutamine and antibiotics were purchased from Invitrogen Life Technologies (CA, USA). All other media and supplements were obtained from Sigma-Aldrich Corporation unless specified otherwise.
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