Cotinine (COT), trans-3′-hydroxycotinine (3-HCOT), cotinine-d3 (COT-d3), trans-3′-hydroxycotinine-d3 (3-HCOT-d3), sodium dodecyl sulfate (SDS) and DSC-18 solid-phase extraction (SPE) powder were purchased from Sigma-Aldrich (St. Louis, MO, USA). Acetonitrile, methanol and water were of liquid chromatography–mass spectrometry (LC–MS) grade and purchased from Fisher Scientific (Fair Lawn, NJ, USA). Sodium hydroxide, magnesium sulfate, sodium chloride and sodium acetate were acquired from Daejung Chemical & Metals Co. (Siheung, Republic of Korea). Primary secondary amine (PSA) bulk sorbent was purchased from Agilent Technologies (Santa Clara, CA, USA). All other reagents were of analytical grade.
Primary secondary amine bulk sorbent
Manufactured by Agilent Technologies
Sourced in United States, Netherlands
Primary secondary amine (PSA) bulk sorbent is a solid phase extraction (SPE) material used for sample preparation and cleanup. It functions as a mixed-mode sorbent, capable of retaining both polar and nonpolar analytes through a combination of hydrophilic and hydrophobic interactions.
Automatically generated - may contain errors
Lab products found in correlation
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Daejung Chemicals (1 mentions)
Sodium acetate, by Daejung Chemicals (1 mentions)
Magnesium sulfate, by Daejung Chemicals (1 mentions)
Sodium chloride (nacl), by Daejung Chemicals (1 mentions)
Sulphuric acid, by Merck Group (1 mentions)
Magnesium sulphate, by Merck Group (1 mentions)
Sigma 4k10 centrifuge, by Merck Group (1 mentions)
Aflatoxins b1, by Merck Group (1 mentions)
N heptane, by Merck Group (1 mentions)
Tween 20, by Merck Group (1 mentions)
Keyhole limpet hemocyanin (klh), by Merck Group (1 mentions)
Chloroform, by Merck Group (1 mentions)
Sodium borohydride, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Dimethylformamide dmf, by Merck Group (1 mentions)
2 protocols using primary secondary amine bulk sorbent
1
Quantitative Bioanalysis of Nicotine Metabolites
2
Quantifying Aflatoxins in Food Samples
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STG, aflatoxins (B1, B2, G1, G2 and M1), acetonitrile (ACN), methanol (MeOH), acetone, chloroform, n-heptane, sulphuric acid, disodium hydrogen phosphate, sodium dihydrogen phosphate, potassium dihydrogen phosphate, sodium carbonate, sodium bicarbonate, sodium chloride, magnesium sulphate, Tween 20, bovine serum albumin (BSA), keyhole limpet hemocyanin (KLH), horseradish peroxidase (HRP), sodium borohydride and dimethylformamide (DMF) were purchased from Sigma-Aldrich (Dorset, UK & Zwijndrecht, the Netherlands). Primary-secondary amine (PSA) bulk sorbent was purchased from Agilent (Amstelveen, the Netherlands). Substrate 3,3',5,5'-tetramethylbenzidine (TMB) for HRP enzyme was obtained from Neogen (Lansing, USA).
Samples were blended using IKA A11 Basic laboratory mill (IKA, Staufen, Germany) and centrifuged in Sigma 4K10 centrifuge (Sigma, Osterode am Harz, Germany). Microtiter plates were read on BioTek EL808 type ELISA plate reader (BioTek, Bad Friedrichshall, Germany).
Samples were blended using IKA A11 Basic laboratory mill (IKA, Staufen, Germany) and centrifuged in Sigma 4K10 centrifuge (Sigma, Osterode am Harz, Germany). Microtiter plates were read on BioTek EL808 type ELISA plate reader (BioTek, Bad Friedrichshall, Germany).
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