Rs 3 5 dhpg

Manufactured by Bio-Techne

Sourced in United Kingdom

The product (RS)-3,5-DHPG is a chemical compound that functions as a selective agonist for metabotropic glutamate receptor 5 (mGlu5). It can be used as a research tool to study the role of mGlu5 receptors in various biological processes.

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Lab products found in correlation

Picrotoxin, by Bio-Techne (3 mentions) Ro 677476, by Bio-Techne (2 mentions) Ifenprodil hemitartrate, by Bio-Techne (2 mentions) D apv, by Bio-Techne (2 mentions) Ly367385, by Bio-Techne (2 mentions) Picrotoxin, by Merck Group (1 mentions) Sulpiride, by Merck Group (1 mentions) β cyclodextrin, by Tokyo Chemical Industry (1 mentions) Quinpirole hydrochloride, by Bio-Techne (1 mentions) Vu0255035, by Bio-Techne (1 mentions) Am251, by Bio-Techne (1 mentions) Npec caged dopamine, by Bio-Techne (1 mentions) Riluzole, by Bio-Techne (1 mentions) Ryanodine, by Bio-Techne (1 mentions) Donkey antimouse alexa fluor 647, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 goat anti guinea pig, by Thermo Fisher Scientific (1 mentions) Kapa mouse genotyping kit, by Roche (1 mentions) Bradford assay, by Bio-Rad (1 mentions) Gf109203x, by Merck Group (1 mentions) Aminooxyacetic acid, by Merck Group (1 mentions)

Spelling variants of this product

(RS)-3,5-dihydroxyphenylglycine (DHPG) (7 citations)

7 protocols using rs 3 5 dhpg

Optogenetic Modulation of Synaptic Signaling

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AAV-shShank3/scrShank3 (AAV5, AAV1; 7.4 × 10¹³ GC/mL; VectorBioLab); AAV-CAG-DIO-tdTomato (AAV9, gift from Prof. Anthony Holtmaat), rAAV5-Ef1a-DIO-hChR2(H134R)-eYFP (5 × 10¹² virus molecules/mL UNC), Ro 677476 (4346, Tocris), D-APV (0106, Tocris), Picrotoxin (1128, Tocris), NBQX (0373, Tocris), (R,S)-3,5-DHPG (0342, Tocris), Ifenprodil hemitartrate (0545, Tocris).

Bariselli S., Tzanoulinou S., Glangetas C., Prévost-Solié C., Pucci L., Viguié J., Bezzi P., O’Connor E.C., Georges F., Lüscher C, & Bellone C. (2016). SHANK3 controls maturation of social reward circuits in the VTA. Nature neuroscience, 19(7), 926-934.

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Preparation of Neuropharmacological Compounds

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Drugs were prepared in a stock solution of water or DMSO and diluted to their final concentration in ACSF. The final concentration of DMSO was <0.1%. β-Cyclodextrin (final concentration <0.001%; TCI America) was used as a carrier for AM251 delivery. (−)-Quinpirole hydrochloride, (RS)-3,5-DHPG, DHβE, VU 0255035, NPEC-caged dopamine, and AM251 were purchased from Tocris Bioscience. (±)-Sulpiride and picrotoxin were purchased from Sigma.

Augustin S.M., Chancey J.H, & Lovinger D.M. (2018). Dual Dopaminergic Regulation of Corticostriatal Plasticity by Cholinergic Interneurons and Indirect Pathway Medium Spiny Neurons. Cell reports, 24(11), 2883-2893.

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Pharmacological Agents for Neuroscience Research

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(RS)-3,5-DHPG, LY367385, MPEP, APV, picrotoxin, ryanodine, riluzole were purchased from Tocris Bioscience. Choline chloride was from Junsei Chemical (Tokyo, Japan). 8-NH₂-cADPR was from Molecular Probes. All other drugs were purchased from Sigma-Aldrich (St Louis, MO, USA). Stock solutions of drugs were made by dissolving in deionized water or DMSO according to manufacturer’s specifications and were stored at − 20 °C. On the day of the experiments, one aliquot was thawed and used. The concentration of DMSO in solutions was maintained at 0.1%.

Yu W., Sohn J.W., Kwon J., Lee S.H., Kim S, & Ho W.K. (2018). Enhancement of dendritic persistent Na+ currents by mGluR5 leads to an advancement of spike timing with an increase in temporal precision. Molecular Brain, 11, 67.

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Radioligand Binding and Western Blotting

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[2,3-³H]D-aspartate (specific activity 11.3 Ci/mmol) and [³H]GABA (specific activity 30.0 Ci/mmol) were from Perkin Elmer (Boston, MA, United States). (±)-baclofen, LY367385, (RS)-3,5 DHPG, and CGP 52432 were purchased from Tocris Bioscience (Bristol, United Kingdom). GF109203X, aminooxyacetic acid, naphtol blue black staining, horseradish peroxidase-coupled anti-mouse and anti-rabbit secondary antibodies were from Sigma (Milan, Italy). Donkey anti-mouse AlexaFluor-647, goat anti-guinea pig AlexaFluor-488, goat anti-rabbit AlexaFluor-555 were from Life Technologies Corporation (Carlsbad, CA, United States). Mouse anti-GABA_B1 and mouse anti-GABA_B2 antibodies were from Santa Cruz Biotechnology (Dallas, TX, United States). Rabbit anti-mGlu1 antibody, used in confocal analysis, was from Abcam (Cambridge, United Kingdom) and mouse anti-mGlu1 monoclonal antibody, used in Western blotting, was from BD Biosciences (San Jose, CA, United States). Guinea pig anti-vesicular glutamate transporters type 1 antibody was from Millipore (Temecula, CA, United States). Guinea pig anti-VGAT was from AlomoneLabs (Jerusalem, Israel). Bradford assay was from Bio-Rad (Segrate, Milan, Italy). KAPA Mouse Genotyping Kits were from Kapa Biosystems (Woburn, MA, United States). ECL Advance^TM was from Amersham Biosciences (Piscataway, NJ, United States).

Vergassola M., Olivero G., Cisani F., Usai C., Bossi S., Puliti A, & Pittaluga A. (2018). Presynaptic mGlu1 Receptors Control GABAB Receptors in an Antagonist-Like Manner in Mouse Cortical GABAergic and Glutamatergic Nerve Endings. Frontiers in Molecular Neuroscience, 11, 324.

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Investigating Synaptic Protein Regulation

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(RS)-3,5-DHPG and dynasore were purchased from Tocris Bioscience (Bristol, UK). AP5 (D-2-amino-5-phosphonopentanoate), MPEP, cholesterol, and methyl-β-cyclodextrin (Mβ-CD) were obtained from Sigma (St. Louis, MO, USA). We used the following antibodies: anti-FMRP (MAB2160; Millipore, Billerica, MA, USA), anti-Cav1 (ab2910, Abcam, Cambridge, UK), anti-GluA1 (ab31232; Abcam), anti-GluA2 (ab20673; Abcam), anti-pan-cadherin (C1821; Sigma), anti-ERK1/2 (sc514302; Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-pERK1/2 (sc81492; Santa Cruz Biotechnology), anti-mTOR (sc517464; Santa Cruz Biotechnology), anti-pmTOR (sc293133; Santa Cruz Biotechnology), and anti-β-actin (A5316; Sigma).

Luo L., Yang L., Zhang K., Zhou S.M., Wang Y., Yang L.K., Feng B., Liu S.B., Wu Y.M., Zhao M.G, & Yang Q. (2023). Caveolin-1-Mediated Cholesterol Accumulation Contributes to Exaggerated mGluR-Dependent Long-Term Depression and Impaired Cognition in Fmr1 Knockout Mice. Molecular Neurobiology, 60(6), 3379-3395.

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Optogenetic Modulation of Synaptic Signaling

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Reagents for Neurotransmission Studies

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Picrotoxin and MK-801 were from Abcam. Sulpiride, JNJ16259685, Clozapine N-oxide, Bafilomycin A1, (RS)-3,5-DHPG and Substance P were obtained from Tocris Bioscience. K-methylsulfate was obtained from Acros Organic. All the other chemicals were from Sigma-Aldrich.

Cai Y., Nielsen B.E., Boxer E.E., Aoto J, & Ford C.P. (2021). Loss of nigral excitation of cholinergic interneurons contributes to Parkinsonian motor impairments. Neuron, 109(7), 1137-1149.e5.

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