Il 6 elisa kit

Manufactured by Solarbio

Sourced in China

The IL-6 ELISA kit is a quantitative sandwich enzyme immunoassay designed for the measurement of Interleukin-6 (IL-6) levels in various biological samples. The kit utilizes the principles of enzyme-linked immunosorbent assay (ELISA) technology.

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Lab products found in correlation

Il 1β elisa kit, by Solarbio (3 mentions) Tnf α elisa kit, by Solarbio (2 mentions) Ldh assay kit, by Nanjing Jiancheng (1 mentions) Il 8 elisa kit, by Solarbio (1 mentions) Rat tnf α elisa kit, by Solarbio (1 mentions) Anti rabbit igg hrp linked antibody, by Cell Signaling Technology (1 mentions) Dmem culture medium, by Thermo Fisher Scientific (1 mentions) Cell counting kit 8 cck 8, by Beyotime (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) Color prestained protein marker, by Thermo Fisher Scientific (1 mentions) F4 80, by Huabio (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Tnf α, by Abcam (1 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (1 mentions) Interleukin 6 (il 6), by Huabio (1 mentions) P nf κb, by Cell Signaling Technology (1 mentions) P ikbα, by Abcam (1 mentions) Nf κb, by Cell Signaling Technology (1 mentions) Multiskan ascent photometric plate reader, by Thermo Fisher Scientific (1 mentions)

6 protocols using il 6 elisa kit

Cytokine Secretion in HK-2 Cells

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The concentration of IL‐1β and IL‐6 in supernatants of HK‐2 cell cultures was evaluated by IL‐1β ELISA Kit (Solarbio) and IL‐6 ELISA Kit (Solarbio), respectively. Absorbance at 490 nm was measured using a Microplate Reader (Pulangxin).

Huang T., Cao Y., Wang H., Wang Q., Ji J., Sun X, & Dong Z. (2020). Circular RNA YAP1 acts as the sponge of microRNA‐21‐5p to secure HK‐2 cells from ischaemia/reperfusion‐induced injury. Journal of Cellular and Molecular Medicine, 24(8), 4707-4715.

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Serum and Liver Biomarkers Evaluation

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The levels of low-density lipids (LDL), high-density lipids (HDL), and pro-inflammatory markers (tumor necrosis factor α (TNF-α), IL-6 and IL-1β) in the rat serum, as well as glutathione (GSH) content, superoxide dismutase (SOD) activity and malondialdehyde (MDA) level in rat liver tissues, were detected by ELISA using LDL ELISA kit (M053549, MREDA), HDL ELISA kit (M053550, MREDA), TNF-α ELISA kit (SEKR-0009, Solarbio), IL-6 ELISA kit (SEKR-0005, Solarbio), IL-1β ELISA kit (SEKR-0002, Solarbio), GSH ELISA kit (F15488, XiTang Biotechnology), SOD ELISA kit (F16742, XiTang Biotechnology), and MDA ELISA kit (F16194, XiTang Biotechnology).

Xu K., Lu G., Feng Q., Chen S, & Wang Y. (2023). Hepatoprotective effect of protocatechuic acid against type 2 diabetes-induced liver injury. Pharmaceutical Biology, 61(1), 737-745.

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Cytokine and Cellular Damage Assays

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The TNF-α, IL-8, and IL-6 levels were determined with the rat TNF-α ELISA kit (Solarbio), IL-8 ELISA kit (Solarbio) and IL-6 ELISA kit (Solarbio) according to the manufacturer’s instructions respectively. LDH release was measured using the LDH assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) according to the manufacturer’s recommendations. Samples were analyzed at 450 nm wavelength by microplate reader.

Shen H., Xie K., Li M., Yang Q, & Wang X. (2022). N6-methyladenosine (m6A) methyltransferase METTL3 regulates sepsis-induced myocardial injury through IGF2BP1/HDAC4 dependent manner. Cell Death Discovery, 8, 322.

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Quantification of Cytokine Levels in Rat Samples

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Take 50 μL of pre-treated rat serum and cell supernatant, add to ELISA microtiter wells and incubate for 1 h, then react with HRP for 30 min, after three washes with PBS for 5 min each time, and add the substrate TMB to develop the color for 15 min. TMB is converted to blue color catalyzed by the HRP enzyme, and converted to the final yellow color in the presence of acid. The absorbance (OD value) was measured at 450 nm with an enzyme marker and the concentration was calculated by standard curve. IL-6 ELISA KIT (Solarbio, SEKH-0013 and SKER-0005), IL-10 ELISA KIT (Solarbio, SEKH-0018 and SKER-0006), IL-1β ELISA KIT (Solarbio, SEKH-0002 and SKER-0002), TNF-α ELISA KIT (Solarbio, SEKH-0047 and SKER-0009).

Zhang L, & Gu X. (2024). 4-hydroxysesamin protects rat with right ventricular failure due to pulmonary hypertension by inhibiting JNK/p38 MAPK signaling. Aging (Albany NY), 16(9), 8142-8154.

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Inflammatory Response Regulation via NF-κB Signaling

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The following reagents were used for examination: lipopolysaccharide (LPS) (Sigma, L3129-10MG), capsaicin (MCE, 404864–50 mg), FeCl₃•6H₂O (Aladdin, F102739-55 g), polyvinylpyrrolidone (PVP) (Aladdin, P434440-250 g), fetal bovine serum (Gibco, 30044333), DMEM culture medium (Gibco, C11995500BT), PBS (Gibco, 8123157), primary and secondary antibody dilution solution (BOSTER, 17K28C17), color pre-stained protein marker (Thermo, 26619), TNF-α (Abcam, ab66579), iNOS (Abcam, ab283655), TGF-β (Huabio, HA7211430), p-NF-κB (Cell Signaling Technology, 3033S), NF-κB (Cell Signaling Technology, 8242T), p-IKBα (Abcam, ab32518), IKBα (Abcam, ab133462), IL-6 (Huabio, R1412-2), β-actin (Cell Signaling Technology, 4970S), Pierce TM BCA protein Assay kit (Thermo Fisher Scientific, Prod # 23227), F4/80 (Huabio, RT1212), anti-rabbit IgG, HRP-linked antibody (Cell Signaling Technology, 7074S), anti-mouse IgG, HRP-linked antibody (Cell Signaling Technology, 7076S), secondary antibody (Thermo Fisher Scientific, Prod # A-31572), secondary antibody (Thermo Fisher Scientific, Prod # A-21202), SuleLumia ECL kit (Abbkine, K22020), TNF-α and iNOS ELISA kit (FineTest, EM0183, EM0272), IL-6 ELISA kit (Solarbio, SEKM-0007), Cell Counting kit-8 (CCK-8, Beyotime, C0038).

Wang R., Li Q., Wu P., Ren K., Li Y., Wang Y., Zhu H, & Lv C. (2024). Fe-Capsaicin Nanozymes Attenuate Sepsis-Induced Acute Lung Injury via NF-κB Signaling. International Journal of Nanomedicine, 19, 73-90.

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Quantitative IL-6 Detection by ELISA

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Quantitative detection of IL-6 was determined by IL-6 ELISA kit (solarbio, Beijing, China). The assay was performed in triplicates, following the manufacturer’s instructions. The plate was read by Thermo Labsystems Multiskan Ascent Photometric plate reader for 96 well plates.

Li L., Li Z., Lu C., Li J., Zhang K., Lin C., Tang X., Liu Z., Zhang Y., Han R., Wang Y., Feng M., Zhuang Y., Hu C, & He Y. (2022). Ibrutinib reverses IL-6-induced osimertinib resistance through inhibition of Laminin α5/FAK signaling. Communications Biology, 5, 155.

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