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Accell system

Manufactured by Horizon Discovery
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The Accell System is a high-throughput screening platform used for gene expression analysis and cell-based assays. It employs a lentiviral delivery method to introduce genetic material into target cells. The system allows for rapid and efficient screening of multiple genetic targets or conditions simultaneously.

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Lab products found in correlation

Accell non targeting control sirna, by Horizon Discovery (1 mentions)

2 protocols using accell system

1

Efficient c-KIT Knockdown by siRNA

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siRNA for c-KIT (cat No. E-003150-00-0005) and Accell Non-Targeting Control siRNA (cat No. D-001910-01-0005) were purchased from Dharmacon. The cells were transfected with siRNA using the Accell System (Dharmacon), cultured for 48 h, and then used in the western blot analysis. To investigate for efficiency of transfection, we used the Accell Green Non-targeting Control siRNA. The efficiency of transfection was over 95% in the cells. More than 98% of the c-KIT proteins were gone by siRNA.
Heo S.K., Noh E.K., Kim J.Y., Jeong Y.K., Jo J.C., Choi Y., Koh S., Baek J.H., Min Y.J, & Kim H. (2017). Targeting c-KIT (CD117) by dasatinib and radotinib promotes acute myeloid leukemia cell death. Scientific Reports, 7, 15278.
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2

Silencing STAT3 in U266B1 Cells

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SiRNA for STAT3 (cat No. E-003544-00-50) and cyclophilin B control siRNA (cat No. D-001910-01-50) were purchased from Dharmacon. U266B1 cells were transfected with siRNA using the Accell SYSTEM (Dharmacon), cultured for 48 h, and then used to measure the diverse proteins by western blotting. To examine for efficiency of transfection, we used Accell Green Non-targeting siRNA. Efficiency of transfection was over the 95% in the U266B1 cells.
Heo S.K., Noh E.K., Seo H.J., Lee Y.J., Koh S., Min Y.J., Choi Y, & Jo J.C. (2022). Radotinib inhibits multiple myeloma cell proliferation via suppression of STAT3 signaling. PLoS ONE, 17(5), e0265958.
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