Rabbit anti bmal1

Manufactured by Cell Signaling Technology

The Rabbit anti-Bmal1 is a primary antibody product that targets the Bmal1 protein. Bmal1 is a key component of the circadian clock, playing a crucial role in the regulation of gene expression in a circadian manner. This antibody can be used to detect and study the Bmal1 protein in various research applications.

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Lab products found in correlation

Rabbit anti cleaved parp, by Cell Signaling Technology (1 mentions) Mouse anti α tubulin, by Merck Group (1 mentions) Rabbit anti phospho chk1, by Cell Signaling Technology (1 mentions) Rabbit anti p21 waf1 cip1, by Cell Signaling Technology (1 mentions) Rabbit anti histone h2ax, by Cell Signaling Technology (1 mentions) Rabbit anti keratin 10, by BioLegend (1 mentions) Rabbit anti phospho p53 ser15, by Cell Signaling Technology (1 mentions) Mouse anti p53 sc 126, by Santa Cruz Biotechnology (1 mentions) Hrp conjugated secondary antibody, by Santa Cruz Biotechnology (1 mentions) Amersham imager 600, by GE Healthcare (1 mentions) Ab76149, by Abcam (1 mentions) Sc 133132, by Santa Cruz Biotechnology (1 mentions) M20005, by Abmart (1 mentions)

2 protocols using rabbit anti bmal1

Western Blot Analysis of Cell Signaling

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Whole cell lysates for Western Blot were prepared with the SDS-sample buffer. Proteins were separated by SDS-PAGE and transferred onto the PVDF membrane (Millipore/Fisher Scientific, Pittsburg, PA), Darmstadt, Germany). Antibodies for immunoblotting from Cell Signaling Technology (Dancers, MA) would include rabbit anti-Bmal1 (#14020), rabbit anti-CLOCK (#5157), rabbit anti-phospho-p53 (Ser15) [#9284], rabbit anti-p21 Waf1/Cip1 (#2947), rabbit anti-Histone H2AX (#2595), rabbit anti-cleaved PARP (#9541), and rabbit anti-phospho-CHK1 (Ser 345) (#2348). Other antibodies include mouse anti-p53 (sc-126; Santa Cruz Biotechnology Inc., Santa Cruz, CA), rabbit anti-Keratin 10 (Poly19054; Biolegend, San Diego, CA), mouse anti-phospho-Histone H2AX (Ser139) (05–636; Millipore/Fisher Scientific), and mouse anti-α-tubulin (T9026; Sigma-Aldrich, St. Louis, MO). HRP-conjugated secondary antibodies were from Santa Cruz Biotechnology Inc. Chemiluminescence images were acquired using an Amersham Imager 600 from GE Healthcare Life Sciences (Pittsburgh, PA) or iBright FL1000 (Invitrogen-Life Technology/Fisher Scientific, Pittsburgh, PA). The level of target proteins was quantified by densitometry scanning with the ImageJ software and normalized to the amount of α-tubulin.

Sun Y., Wang P., Li H, & Dai J. (2018). BMAL1 and CLOCK proteins in regulating UVB-induced apoptosis and DNA damage responses in human keratinocytes. Journal of cellular physiology, 233(12), 9563-9574.

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Investigating Eukaryotic Protein Regulation

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The primary antibodies used are: mouse anti-eIF2α (sc-133132 Santa Cruz Biotechnology, 1:1000 for western blot); rabbit anti-phospho-eIF2α (9721 Cell Signaling Technology, 1:1000 for western blot); rabbit anti-BMAL1(14020 Cell Signaling Technology, 1:1000 for western blot); mouse anti-GAPDH (60004 Proteintech, 1:1000 for western blot); mouse anti-β-tubulin (M20005, Abmart; 1:3000 for western blot); rabbit anti-PABP1 (ab2060, Abcam, 1:100 for immunofluorescence), rabbit anti-YB1 (ab76149, Abcam, 1:200 for immunofluorescence); and rabbit anti-cleaved-caspase-3 (9661 Cell Signaling Technology, 1:200 for immunofluorescence). The drug used was sodium arsenite (S7400-100G, Sigma, 100 mg/kg for mouse and 20 or 50 μM for cells).

Wang R., Jiang X., Bao P., Qin M, & Xu J. (2019). Circadian control of stress granules by oscillating EIF2α. Cell Death & Disease, 10(3), 215.

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