Vinblastine

Manufactured by Bio-Techne

Sourced in United States

Vinblastine is a lab equipment product manufactured by Bio-Techne. It is a plant-derived compound used for research purposes in various scientific fields.

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Lab products found in correlation

Cisplatin, by Bio-Techne (3 mentions) Docetaxel, by Bio-Techne (3 mentions) Paclitaxel, by Bio-Techne (3 mentions) Vincristine, by Bio-Techne (3 mentions) Verapamil, by Merck Group (3 mentions) Colchicine, by Bio-Techne (2 mentions) Mitoxantrone, by Bio-Techne (2 mentions) 3 4 5 dimethylthiazol yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (2 mentions) Monoclonal antibody against gapdh, by Cell Signaling Technology (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions) To901317, by Cayman Chemical (1 mentions) Ginkgolide a, by Santa Cruz Biotechnology (1 mentions) Ginkgolideb, by Santa Cruz Biotechnology (1 mentions) 3h paclitaxel 37.9 ci mmol, by Moravek Biochemicals (1 mentions) Nvp bhg712, by Novartis (1 mentions) Nigericin, by InvivoGen (1 mentions)

7 protocols using vinblastine

Quantifying Ligand Binding to PXR

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Vinblastine was obtained from Tocris Bioscience
(Minneapolis, MN, USA); GST-hPXR-LBD, LanthaScreen Tb-anti-GST antibody,
TR-FRET PXR (SXR) assay buffer, BODIPY FL Vinblastine, and 1 M DTT
(dithiothreitol) were purchased from Invitrogen (Carlsbad, CA, USA);
human glutathione S transferase protein (GST) was purchased from Abcam
(Cambridge, MA, USA); catharanthine and vindoline were purchased from
LKT Laboratories, Inc. (St. Paul, MN, USA); deacetyl Vinblastine was
purchased from Toronto Research Chemicals, Inc. (Toronto, Ontario,
Canada); dimethyl sulfoxide (DMSO) was purchased from Fisher Scientific
(Pittsburgh, PA, USA); TO901317 was purchased from Cayman Chemical
(Ann Arbor, MI, USA); SR12813 was purchased from Enzo Life Sciences
(Farmingdale, NY, USA); clotrimazole, rifampicin, 2-amino-2-(hydroxymethyl)-1,3-propanediol
(Tris), potassium chloride (KCl), 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate
hydrate (CHAPS), and bovine serum albumin (BSA) were purchased from
Sigma (St. Louis, MO, USA); hyperforin, ginkgolide A, and ginkgolide
B were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA);
and black 384-well polypropylene plates were purchased from Matrical
Bioscience (Spokane, WA, USA).

Lin W., Liu J., Jeffries C., Yang L., Lu Y., Lee R.E, & Chen T. (2014). Development of BODIPY FL Vindoline as a Novel and High-Affinity Pregnane X Receptor Fluorescent Probe. Bioconjugate Chemistry, 25(9), 1664-1677.

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Paclitaxel Resistance Mechanism Evaluation

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[³H]-Paclitaxel (37.9 Ci/mmol) was purchased from Moravek Biochemicals, Inc. (Brea, CA). Dulbecco modified Eagle medium (DMEM), fetal bovine serum (FBS), phosphate buffer saline (PBS), 10,000 IU/ml penicillin and 10,000 μg/ml streptomycin, and trypsin 0.25% were purchased from Hyclone (Waltham, MA). Monoclonal antibody against GAPDH was purchased from Cell Signaling Technologies (Beverly, MA). The polyclonal antibody PA5-23652 for ABCC10 was obtained from Thermo scientific (Rockford, IL). Monoclonal antibody 3D7G8 against EphB4 was obtained from Life technologies, Invitrogen (New York, NY). NVP-BHG712 was a gift from Novartis (Basel, Switzerland). Cepharanthine was generously donated by Kakenshoyaku Co. (Tokyo, Japan). PAK-104P was a gift from Nissan Chemical Industries (Tokyo, Japan). Paclitaxel, docetaxel, colchicine, mitoxantrone, vincristine, vinblastine and cisplatin were purchased from Tocris Bioscience (Ellisville, MO). 3-(4, 5-Dimethylthiazol-yl)-2,5-diphenyltetrazolium bromide (MTT), Dimethyl sulfoxide (DMSO) and verapamil were obtained from Sigma-Aldrich Co. (St. Louis, MO).

Kathawala R.J., Wei L., Anreddy N., Chen K., Patel A., Alqahtani S., Zhang Y.K., Wang Y.J., Sodani K., Kaddoumi A., Ashby CR J.r, & Chen Z.S. (2014). The small molecule tyrosine kinase inhibitor NVP-BHG712 antagonizes ABCC10-mediated paclitaxel resistance: a preclinical and pharmacokinetic study. Oncotarget, 6(1), 510-521.

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Signaling Pathway Modulation Protocol

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Brefeldin A and rapamycin was purchased from LC Labs. Golgicide A was from Sigma Aldrich. Gramicidin was from Molecular Probes. Nigericin, ruxolitinib, and tofacitinib were from Invivogen. Vinblastine was from Tocris. Human recombinant IFNβ and IFNγ were obtained from Millipore.

Gregory D.J., DeLoid G.M., Salmon S.L., Metzger D.W., Kramnik I, & Kobzik L. (2020). SON DNA-Binding Protein mediates macrophage autophagy and responses to intracellular infection. FEBS letters, 594(17), 2782-2799.

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Paclitaxel and Associated Reagents

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[³H]-Paclitaxel (25.7 Ci/mmol) was purchased from Moravek Biochemicals, Inc. (Brea, CA) Dulbecco modified Eagle medium (DMEM), Iscove’s DMEM, fetal bovine serum (FBS), phosphate buffer saline (PBS), 10,000 IU/ml penicillin and 10,000 μg/ml streptomycin, and trypsin 0.25% were purchased from Hyclone (Waltham, MA). Monoclonal antibody against GAPDH was purchased from Cell Signaling Technologies (Beverly, MA). Antibody D-19 against ABCC10 was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Monoclonal antibody Ab81 against c-Kit and monoclonal antibody D12E12 against phospho-c-Kit (p-c-Kit) were obtained from Cell Signaling Technologies (Beverly, MA). Masitinib was a gift from AB Science (Paris, France). Cepharanthine was generously given by Kakenshoyaku Co. (Tokyo, Japan). PAK-104P was a gift from Nissan Chemical Industries (Tokyo, Japan). Paclitaxel, docetaxel, vincristine, vinblastine and cisplatin were purchased from Tocris Bioscience (Ellisville, MO). Boron-dipyrromethene (BODIPY) FL (fluorescent) Paclitaxel was purchased from Life technologies, Invitrogen (New York, NY). 3-(4, 5-Dimethylthiazol-yl)-2,5-diphenyltetrazolium bromide (MTT), Dimethyl sulfoxide (DMSO) and verapamil were obtained from Sigma-Aldrich Co. (St. Louis, MO).

Kathawala R.J., Sodani K., Chen K., Patel A., Abuznait A.H., Anreddy N., Sun Y.L., Kaddoumi A., Ashby CR J.r, & Chen Z.S. (2014). Masitinib Antagonizes ATP-Binding Cassette Subfamily C Member 10-Mediated Paclitaxel Resistance: A Preclinical Study. Molecular cancer therapeutics, 13(3), 714-723.

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Paclitaxel Resistance Assay Protocol

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[³H]-Paclitaxel (37.9 Ci/mmol/L) was purchased from Moravek Biochemicals, Inc. DMEM, FBS, 0.25% trypsin, and penicillin/streptomycin were products of HyClone, Thermo Scientific. Antibody C-219 against ABCB1 was purchased from Signet Laboratories, Inc. Antibodies D-19 against ABCC10 and β-actin were obtained from Santa Cruz Biotechnology, Inc. Ibrutinib was a gift from Johnson & Johnson Services, Inc. Paclitaxel, docetaxel, vincristine, vinblastine, colchicine, cisplatin, and mitoxantrone were purchased from Tocris Bioscience. Cepharanthine was a gift from Kakenshoyaku Co. 3-(4, 5-Dimethylthiazol-yl)-2,5-diphenyltetrazolium bromide (MTT), DMSO, verapamil, and other chemicals were obtained from Sigma Chemical. Fumitremorgin C (FTC) was synthesized by Thomas McCloud Developmental Therapeutics Program, Natural Products Extraction Laboratory (NIH, Bethesda, MD).

Zhang H., Patel A., Wang Y.J., Zhang Y.K., Kathawala R.J., Qiu L.H., Patel B.A., Huang L.H., Shukla S., Yang D.H., Ambudkar S.V., Fu L.W, & Chen Z.S. (2017). The BTK Inhibitor Ibrutinib (PCI-32765) Overcomes Paclitaxel Resistance in ABCB1- and ABCC10-Overexpressing Cells and Tumors. Molecular cancer therapeutics, 16(6), 1021-1030.

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Inhibitor Sources for Cell Studies

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Inhibitors were purchased from the following distributors: AdooQ BioScience (Irvine, CA, USA; Aurora A inhibitor I, BI‐2536, epothilone A, MK‐1775 and VX‐680); Axon Medchem (Reston, VA, USA; SPL‐B); Focus Biomolecules (Plymouth Meeting, PA, USA; Latrunculin A); MedChemExpress (Princeton, NJ, USA; PF‐2771); Selleck Chemicals (Houston, TX, USA; GSK‐923295); Sigma‐Aldrich (Importazole, RO‐3306, and S‐trityl‐l‐cysteine); Thermo Fisher Scientific (Waltham, MA, USA; Colcemid (KaryoMAX Colcemid)); Calbiochem (San Diego, California CA, USA; Etoposide); LKT Laboratories (St. Paul, MN, USA; Vinblastine); Tocris Bioscience, (Bristol, UK; Monastrol); Wako (Cytochalasin B, daunorubicin, doxorubicin, nocodazole and paclitaxel).

Yoshizawa K., Matsura A., Shimada M., Ishida‐Ishihara S., Sato F., Yamamoto T., Yaguchi K., Kawamoto E., Kuroda T., Matsuo K., Tamaoki N., Sakai R., Shimada Y., Mishra M, & Uehara R. (2023). Tetraploidy‐linked sensitization to CENP‐E inhibition in human cells. Molecular Oncology, 17(6), 1148-1166.

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Curcumin and Cytotoxic Agents Protocol

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Curcumin (> 97% purity) was purchased from Thermo Fisher Scientific (Waltham, MA). We prepared a 100 mM stock solution of Curcumin in DMSO. Doxorubicin and Vinblastine were purchased from Tocris (Minneapolis, MN, USA), whereas Bleomycin was purchased from Tokyo Chemical Industry Co., Ltd (Portland, OR, USA). Stearic acid, lecithin, chloroform, Myrj59 and TPGS was purchased from Millipore-Sigma (St Louis, MO, USA). All general solvents and reagents were of HPLC grade or the highest grade commercially available.

Guorgui J., Wang R., Mattheolabakis G, & Mackenzie G.G. (2018). Curcumin formulated in solid lipid nanoparticles has enhanced efficacy in Hodgkin's lymphoma in mice. Archives of biochemistry and biophysics, 648.

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