Mip 3β ccl19

Manufactured by Thermo Fisher Scientific

Sourced in United Kingdom, United States

MIP-3β (CCL19) is a chemokine that plays a role in the migration and homing of lymphocytes. It is involved in the recruitment of dendritic cells and lymphocytes to sites of inflammation and lymphoid tissues.

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Lab products found in correlation

Gateway cloning technology, by Thermo Fisher Scientific (2 mentions) Zstk474, by Selleck Chemicals (2 mentions) Cd4 fitc, by Cytek Biosciences (1 mentions) Cd25 apc, by Miltenyi Biotec (1 mentions) Cd8 pecy7, by Cytek Biosciences (1 mentions) Ghost red 780 viability dye, by Cytek Biosciences (1 mentions) Cd4 percp cy5, by Miltenyi Biotec (1 mentions) 7 aminoactinomycin d 7 aad, by BD (1 mentions) Live dead fixable yellow dead cell stain, by Thermo Fisher Scientific (1 mentions) Cd69 fitc, by BD (1 mentions) Ccr7 pe, by BioLegend (1 mentions) Tcrβ apc cy7, by BioLegend (1 mentions) Cd11c bv421, by BD (1 mentions) Cd3 fitc, by Cytek Biosciences (1 mentions) Cd4 pe cy7, by Cytek Biosciences (1 mentions) Cd11c fitc, by Miltenyi Biotec (1 mentions) Sdf 1α cxcl12, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

CCL19 (54 citations) MIP-3β (2 citations) Murine MIP-3β (CCL19) (2 citations)

4 protocols using mip 3β ccl19

Characterization of Akt and PI3K Inhibitors

Cited 1 time

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OVA_323-339 peptide was synthesised by Southampton Polypeptides. IC87114 was synthesised as described previously.^{14 (link), 60 (link)} Akt inhibitor VIII (Akti ½) was purchased from Merck Millipore (Billerica, MA, USA) and used at 2 μm. ZSTK-474 was purchased from Selleck (Houston, TX, USA) and used at 100 nM. The different glutathione S-transferase (GST) fusion proteins containing the CRIB (Cdc42/Rac1 interactive binding) domain of PAK (GST-PAK-CRIB), the Rho-binding domain (RBD) of Rhotekin (GST-Rhotekin-RBD) or the Ras-binding domain (RBD) of RalGDS (GST-Ral-RBD) were a gift from Heidi Welch (Babraham Institute). HA-Rap1V12 was cloned into MIGR-1-IRES-GFP using the Gateway cloning technology from Life technologies (Grand Island, NY, USA). The human p110δ retroviral construct was described previously.^{61 (link)} The recombinant murine MIP-3β (CCL19) was obtained from Peprotech (London, UK).

Garçon F, & Okkenhaug K. (2016). PI3Kδ promotes CD4+ T-cell interactions with antigen-presenting cells by increasing LFA-1 binding to ICAM-1. Immunology and Cell Biology, 94(5), 486-495.

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Flow Cytometry Analysis of Immune Cells

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Antibodies and reagents used for flow cytometry were as follows: CD11c-BV421 (585452), MHCII-fluorescein isothiocyanate (FITC) (553623), 7-aminoactinomycin D (7AAD) (51-68981E), CD69-FITC (553236), CD11c-phycoerythrin (PE) (557401), and anti-mouse CD16/CD32 (Fc block) (553142) provided by Becton Dickinson (BD); CD40-allophycocyanin (APC) (20-8050-U025), CD44-V450 (75-0441-U025), CD4-PECy7 (60-0041-U100), CD45.1-PerCPCy5 (65-0453-U500), CD3-FITC (35-0031-U500), CD8-PECy7 (60-0081), and Ghost Red 780 Viability Dye (13-0865-T100) from Tonbo Biosciences; CD4-FITC (130-109-498), CD62L-PerCP-Vio700 (130-107-072), CD25-APC (130-109-052), CD4-PerCPCy5.5 (130-109-497), and CD11c-FITC (130-102-466) provided by Miltenyi; CCR7-PE (120105) and TCRβ-APCCy7 (109220) from BioLegend; and AbC RH capture beads (A10389) and LIVE/DEAD Fixable Yellow Dead Cell Stain (L34968) from Invitrogen. In addition, cell dyes 5-(and-6)-(((4-chloromethyl)benzoyl)amino)tetramethylrhodamine (CMTMR) (C2927) and carboxyfluorescein diacetate succinimidyl ester (CFSE) (C34554) were obtained from Thermo Fisher Scientific, and recombinant murine chemokine MIP-3β (CCL19) (250-27B) was provided by Peprotech.

Alcaraz-Serna A., Bustos-Morán E., Fernández-Delgado I., Calzada-Fraile D., Torralba D., Marina-Zárate E., Lorenzo-Vivas E., Vázquez E., de Alburquerque J.B., Ruef N., Gómez M.J., Sánchez-Cabo F., Dopazo A., Stein J.V., Ramiro A, & Sánchez-Madrid F. (2021). Immune synapse instructs epigenomic and transcriptomic functional reprogramming in dendritic cells. Science Advances, 7(6), eabb9965.

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Detailed Protein Reagent Synthesis and Usage

Cited 1 time

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OVA_323-339 peptide was synthesized by Southampton Polypeptides. IC87114 was synthesized as described previously^{14 (link), 60 (link)}. Akt inhibitor VIII (Akti ½) was purchased from Merck Millipore (Billerica, MA) and used at 2 μM. ZSTK-474 was purchased from Selleck (Houston, TX) and used at 100 nM. The different GST fusion proteins containing the Cdc42/Rac1 interactive binding (CRIB) domain of PAK (GST-PAK-CRIB), the Rho binding domain (RBD) of Rhotekin (GST-Rhotekin-RBD) or the Ras binding domain (RBD) of RalGDS (GST-Ral-RBD) were a gift from Heidi Welch (Babraham Institute). HA-Rap1V12 was cloned into MIGR-1-IRES-GFP using the Gateway cloning technology from Life technologies (Grand Island, NY). The human p110δ retroviral construct was described previously^{61 (link)}. The recombinant murine MIP-3β (CCL19) was obtained from Peprotech (London, UK).

Garçon F, & Okkenhaug K. (2016). PI3Kδ promotes CD4+ T-cell interactions with antigen-presenting cells by increasing LFA-1 binding to ICAM-1. Immunology and Cell Biology, 94(5), 486-495.

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Transwell Assay for DC Migration

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Migration was assessed in vitro using a transwell system (24-well, pore size 5 μm polycarbonate inserts; Corning Costar, USA). 1.5 × 10⁵ DCs from RA patients were seeded in the upper chamber and AIM-V medium alone or supplemented with 250 ng/ml of SDF-1α/CXCL12 or MIP-3β/CCL19 (PeproTech, Rocky Hill, CT, USA) was added to the lower chamber. After 4-h incubation at 37°C and 5% CO₂, DCs in the lower chamber were counted by flow cytometry. DCs migration is expressed as “migration index” (migration toward chemokines/migration toward medium).

García-González P.A., Schinnerling K., Sepúlveda-Gutiérrez A., Maggi J., Hoyos L., Morales R.A., Mehdi A.M., Nel H.J., Soto L., Pesce B., Molina M.C., Cuchacovich M., Larrondo M.L., Neira Ó., Catalán D.F., Hilkens C.M., Thomas R., Verdugo R.A, & Aguillón J.C. (2016). Treatment with Dexamethasone and Monophosphoryl Lipid A Removes Disease-Associated Transcriptional Signatures in Monocyte-Derived Dendritic Cells from Rheumatoid Arthritis Patients and Confers Tolerogenic Features. Frontiers in Immunology, 7, 458.

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