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Clear strategy screens 1

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The Clear Strategy screens #1 are designed for laboratory use. The core function of these screens is to provide a clear and unobstructed viewing surface for various applications within the research environment.

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Lab products found in correlation

Crystal screen ht, by Hampton Research (2 mentions) Wizard precipitant synergy block 2, by Molecular Dimensions (2 mentions)

2 protocols using clear strategy screens 1

1

Protein Complex Crystallization Conditions

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Complexes of iv1 scFv/iGL-VRC01 Fab, iv4-HL Fab/iGL-VRC01 Fab, iv9 Fab/iGL-VRC01 Fab and iv12 Fab/iGL-12A21Fab were concentrated to ~4.5, ~9, ~10, and ~10 mg/mL, respectively (Table S3). Complexes were screened using commercially available screens: Rigaku Wizard Precipitant Synergy block #2, Molecular Dimensions Proplex HT-96, Clear Strategy screens #1, and Hampton Research Crystal Screen HT; using vapor diffusion method. An NT8 dispensing robot, and Rock Imager was used to set up and screen crystallization conditions. Crystallization conditions were as follows: 0.1 M HEPES pH 7.0, PEG 8000 for iv1 scFv/iGL-VRC01 Fab; 0.1 M Tris pH 7.5, 8, %w/v PEG 20K, 8%w/v PEG MME 550, 0.2 M KSCN for iv4-HL Fab/iGL-VRC01 Fab; 0.08% Sodium Acetate pH 3.5, 24% PEG 4000, 0.16M Ammonium Acetate for iv9 Fab/iGL-VRC01 Fab and 0.1M KCL, 0.1 M Tris pH8, and 15%w/v PEG MME 2 K for iv12 Fab/iGL-12A21 Fab.
Seydoux E., Wan Y.H., Feng J., Wall A., Aljedani S., Homad L.J., MacCamy A.J., Weidle C., Gray M.D., Brumage L., Taylor J.J., Pancera M., Stamatatos L, & McGuire A.T. (2021). Development of a VRC01-class germline targeting immunogen derived from anti-idiotypic antibodies. Cell reports, 35(5), 109084.
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2

Protein Complex Crystallization Conditions

Check if the same lab product or an alternative is used in the 5 most similar protocols
Complexes of iv1 scFv/iGL-VRC01 Fab, iv4-HL Fab/iGL-VRC01 Fab, iv9 Fab/iGL-VRC01 Fab and iv12 Fab/iGL-12A21Fab were concentrated to ~4.5, ~9, ~10, and ~10 mg/mL, respectively (Table S3). Complexes were screened using commercially available screens: Rigaku Wizard Precipitant Synergy block #2, Molecular Dimensions Proplex HT-96, Clear Strategy screens #1, and Hampton Research Crystal Screen HT; using vapor diffusion method. An NT8 dispensing robot, and Rock Imager was used to set up and screen crystallization conditions. Crystallization conditions were as follows: 0.1 M HEPES pH 7.0, PEG 8000 for iv1 scFv/iGL-VRC01 Fab; 0.1 M Tris pH 7.5, 8, %w/v PEG 20K, 8%w/v PEG MME 550, 0.2 M KSCN for iv4-HL Fab/iGL-VRC01 Fab; 0.08% Sodium Acetate pH 3.5, 24% PEG 4000, 0.16M Ammonium Acetate for iv9 Fab/iGL-VRC01 Fab and 0.1M KCL, 0.1 M Tris pH8, and 15%w/v PEG MME 2 K for iv12 Fab/iGL-12A21 Fab.
Seydoux E., Wan Y.H., Feng J., Wall A., Aljedani S., Homad L.J., MacCamy A.J., Weidle C., Gray M.D., Brumage L., Taylor J.J., Pancera M., Stamatatos L, & McGuire A.T. (2021). Development of a VRC01-class germline targeting immunogen derived from anti-idiotypic antibodies. Cell reports, 35(5), 109084.
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