For all experiments, male mice of strain DBA/1 purchased from Janvier Labs were used. Young adults from an age of 8 weeks up to a maximum age of 30 weeks were used for the experiments. The in-house experiments using DBA/1 were approved by the responsible animal ethics committee of the state of Saxony-Anhalt, Germany (Landesverwaltungsamt Sachsen-Anhalt, Department of Consumer Protection and Veterinary Affairs, Halle (Saale), Saxony-Anhalt, Germany) under the following approval number: 42502-2-1472 MLU. We confirm that all animal handling procedures were carried out in accordance with directive 2010/63/EU of the European Parliament and of the Council on the protection of animals used for scientific purposes, the German Animal Protection Act and recommendations from the Federation of European Laboratory Animal Science Associations (FELASA).
Throughout the experiment, the mice were housed in individually ventilated cages (IVC, type II long, Tecniplast) with 5 mice per cage. The standardized housing conditions (room temperature: 22 °C ± 2 °C, humidity: 50% ± 20%) were controlled by a central ventilation system. The regulation of the day-night-rhythm (12 h day/12 h night) took place via a timer. The animals were given standard mouse food (ssniff: mouse breeding) and tap water with drinking water quality ad libitum.
Throughout the experiment, the mice were housed in individually ventilated cages (IVC, type II long, Tecniplast) with 5 mice per cage. The standardized housing conditions (room temperature: 22 °C ± 2 °C, humidity: 50% ± 20%) were controlled by a central ventilation system. The regulation of the day-night-rhythm (12 h day/12 h night) took place via a timer. The animals were given standard mouse food (ssniff: mouse breeding) and tap water with drinking water quality ad libitum.