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Pw8810

Manufactured by Enzo Life Sciences
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Sourced in Switzerland

The PW8810 is a laboratory microplate washer designed for automated washing of microplates. It can accommodate a variety of microplate formats and has adjustable washing parameters to suit different applications.

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Lab products found in correlation

A11004, by Thermo Fisher Scientific (2 mentions) Proteinase and phosphatase inhibitor, by Thermo Fisher Scientific (1 mentions) Ab110413, by Abcam (1 mentions) A2228, by Merck Group (1 mentions) Z0334, by Agilent Technologies (1 mentions) Ab91526, by Abcam (1 mentions) Odyssey image system, by LI COR (1 mentions) Ma1 045, by Thermo Fisher Scientific (1 mentions) Irdye secondary antibody, by LI COR (1 mentions) A21236, by Thermo Fisher Scientific (1 mentions) A 21245, by Thermo Fisher Scientific (1 mentions) A21247, by Thermo Fisher Scientific (1 mentions) A11001, by Thermo Fisher Scientific (1 mentions) Ab2864, by Abcam (1 mentions) Acc 003, by Alomone (1 mentions) Ab4080, by Abcam (1 mentions) A2066, by Merck Group (1 mentions) Sc 126, by Santa Cruz Biotechnology (1 mentions) F7425, by Merck Group (1 mentions) F3165, by Merck Group (1 mentions)

Close spelling variants of this product

BML-PW8810 (25 citations) BML-PW8810-0100 (7 citations) BML-PW8810-0500 (4 citations) Ubiquitin (BML-PW8810 (3 citations) Anti-ubiquitin (BML-PW8810; (2 citations)

5 protocols using pw8810

1

Synchronizing T. gondii Infection in HFF Cells

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The day before the infection, type II parasites were passaged onto new HFFs to obtain parasites with a high viability. T. gondii were prepared from HFF cultures that had been freshly syringe lysed with a 25-gauge syringe. Parasites were subsequently syringe lysed twice with a 27-gauge syringe, and excess HFF cell debris was removed by centrifugation. Then, the parasites were added to the experimental cells at an MOI of 2. The cell cultures with added T. gondii were then centrifuged at 500 × g for 5 min to synchronize the infection, and the cultures were incubated at 37°C and 5% CO2 for 3 h. Samples treated with IFN-γ were subjected to 100 IU/ml human IFN-γ (285-IF; R&D Systems) for 18 h prior to infection. Upon fixation, cells were stained with Hoechst 33342 and mouse anti-ubiquitin monoclonal antibody (MAb) FK2 (PW8810; Enzo Lifesciences; RRID AB_10541840) and Alexa Fluor 568-conjugated secondary goat anti-mouse antibody (A-11004; Invitrogen; RRID AB_141371).
Yakimovich A., Huttunen M., Samolej J., Clough B., Yoshida N., Mostowy S., Frickel E.M, & Mercer J. (2020). Mimicry Embedding Facilitates Advanced Neural Network Training for Image-Based Pathogen Detection. mSphere, 5(5), e00836-20.
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2

Protein Extraction and Western Blot Analysis in Mice

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Mice of each genotype were anesthetized, and the cortex was dissected, quickly frozen at –80 °C, homogenized and sonicated in RIPA buffer with proteinase and phosphatase inhibitors (Thermo Fisher, catalog no. 89900). The protein extract was centrifuged to remove tissue debris, boiled for 15 min, subjected to 10% SDS-PAGE and transferred to a western blot membrane. The membrane was incubated with primary antibodies against PSD-95 (MA1–045, Thermo Fisher; 1:1,000), GFAP (Z0334, Dako; 1:2,000), ATP5A1 (14748, Abcam; 1:1,000), ATP5h (173006, Abcam; 1:1,000), OXPHOS (ab110413, Abcam; 1:1,000), DLP1 (61112, BD; 1:1,000), OPA1 (80471, Cell Signaling; 1:1,000), ubiquitinylated conjugation (PW8810, Enzolifescience; 1:1,000), p62 (ab91526, Abcam; 1:1,000), β-tubulin (T3592–100UG, Sigma; 1:1,000), GAPDH (10494–1-AP, Proteintech; 1:1,000) and actin (A2228, Sigma-Aldrich; 4967, Cell Signaling; 1:1,000) overnight at 4 °C and then with IRDye secondary antibodies (LI-COR Bioscience) for 1 h at room temperature and analyzed with a LI-COR Odyssey image system.
Choi S.Y., Lopez-Gonzalez R., Krishnan G., Phillips H.L., Li A.N., Seeley W.W., Yao W.D., Almeida S, & Gao F.B. (2019). C9ORF72-ALS/FTD-associated poly(GR) binds Atp5a1 and compromises mitochondrial function in vivo. Nature neuroscience, 22(6), 851-862.
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3

Antibody Profiling for Toxoplasma Infection

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Antibodies used in this study were rabbit pAb anti-p62 (#PM045, MBL; RRID:AB_1279301), mouse mAb anti-GRA2 (A1298, Biovision) mouse mAb anti-ubiquitin FK2 (PW8810, Enzo Lifesciences; RRID:AB_10541840), mouse mAb anti-SAG1 (home-made) and rat mAb anti-HA (11867423001, Sigma). Secondary antibodies used were Alexa Fluor 647-conjugated goat anti-rabbit (A-21245, Invitrogen; RRID:AB_141775), anti-rat (A-21247, Invitrogen; RRID:AB_141778) or anti-mouse (A-21236, Invitrogen; RRID:AB_141725), Alexa Fluor 488-conjugated goat anti-mouse (A-11001, Invitrogen; RRID:AB_2534069) and Alexa Fluor 568-conjugated goat anti-mouse (A-11004, Invitrogen; RRID:AB_141371).
Fisch D., Yakimovich A., Clough B., Wright J., Bunyan M., Howell M., Mercer J, & Frickel E. (2019). Defining host–pathogen interactions employing an artificial intelligence workflow. eLife, 8, e40560.
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4

Antibody Detection and Quantification Protocol

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Antibody against Cav1.2 (ACC003; Alomone, Jerusalem, Israel) was used at a dilution of 1/200. Antibody against β2b (ab54920; Abcam, Cambridge, UK) was used at a dilution of 1/200. Antibody against α2δ-1 (ab2864; Abcam, Cambridge, UK) was used at a dilution of 1/1,000. FK2 antibody was used at the dilution of 1/500 (PW8810, Enzo Life Sciences, Lausanne, Switzerland). USP2 antibody used at a dilution of 1/500 was a gift from Prof. O. Staub (Department of Pharmacology and Toxicology, Lausanne, Switzerland). Monoclonal antibody raised against actin was purchased from Sigma-Aldrich (Sigma-Aldrich Chemie, Postfach, Switzerland) and used at a dilution of 1/1,000. Antibody raised against S-tag was purchased from Abcam (ab18588; Abcam, Cambridge, UK) and used at a dilution of 1/200.
Rougier J.S., Albesa M., Syam N., Halet G., Abriel H, & Viard P. (2014). Ubiquitin-specific protease USP2-45 acts as a molecular switch to promote α2δ-1-induced downregulation of Cav1.2 channels. Pflugers Archiv, 467(9), 1919-1929.
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5

Antibody Use in Protein Analysis

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Antibodies used in this study were: mouse anti-β-actin (ab6276, Abcam), rabbit anti-actin (A2066, Sigma-Aldrich), anti-USP7 (ab4080, Abcam), anti-S18 phosphorylated USP7 (ABC225, Merck-Millipore), anti-S18 non-phosphorylated USP7 (ABC226, Merck-Millipore), anti-USP11 (A301-613A, Bethyl Laboratories), anti-TRIM27 (12205-1-AP, Proteintech, or NBP2-46214, Novus Biologicals), anti-TCEAL4 (SAB1400709, Sigma-Aldrich, or PA567536, Invitrogen, Thermo Fisher Scientific), anti-TCEAL1 (sc-393621, Santacruz), anti-p53 (sc-126, Santacruz), anti-FLAG (F3165 or F7425, Sigma-Aldrich), anti-Ub (PW8810, Enzo Life Sciences) and anti-SUMO1 (#4930, Cell Signaling, or 33-2400, Invitrogen). Polyclonal affinity-purified sheep anti-GFP was a gift from Ian Prior (Liverpool, UK).
Querques F., Darling S., Cheetham‐Wilkinson I.J., Kim R.Q., Hapangama D.K., Sixma T.K., & Coulson J.M. (2021). S18-phosphorylation of USP7 regulates interaction with TCEAL4 that defines specific complexes and potentially distinct functions.
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