Licor 10x orange dye

Manufactured by Bio-Rad

The LICOR 10X orange dye is a concentrated stock solution designed for use in molecular biology applications. It is a stable, highly pigmented dye that can be used to visualize nucleic acids and proteins in gel electrophoresis. The dye is formulated to provide a consistent and reliable performance in various experimental procedures.

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Lab products found in correlation

Odyssey infrared imager, by LI COR (2 mentions) Tbe gel, by Bio-Rad (2 mentions)

2 protocols using licor 10x orange dye

EMSA Analysis of STAT1-DNA Complexes

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IRDye labeled (5′ end) sense and anti-sense GAS (sense sequence: 5′-GATGTATTTCCCAGAAAAGG-3′ found upstream of the Fc-Gamma Receptor gene^{47 (link)}), and ISRE (sense sequence: 5′-GGGAAAGGGAAACCGAAACTGAAGCC-3′, found upstream of the ISG15 gene^{48 (link)}), DNA oligonucleotides were synthesized and annealed by Integrated DNA Technologies. DNA probes (0.05 pmole dsDNA per 20 µl reaction volume) were combined ±60 pmole STAT1 dimer in a total 20 μl reaction volume (EMSA buffer: 10 mM Tris pH 7.5, 50 mM KCl, 10 mM DTT, 0.125% Tween-20, 0.05 µg poly (dI·dC), 0.025 µg salmon sperm DNA) for 10 min at room temperature. Purified TgIST proteins were added to STAT1-DNA complexes and incubated for 10 min. Samples were mixed with LICOR 10X orange dye, resolved by 5% TBE gel (Bio-Rad), and visualized using an Odyssey infrared imager (LI-COR Biosciences).

Huang Z., Liu H., Nix J., Xu R., Knoverek C.R., Bowman G.R., Amarasinghe G.K, & Sibley L.D. (2022). The intrinsically disordered protein TgIST from Toxoplasma gondii inhibits STAT1 signaling by blocking cofactor recruitment. Nature Communications, 13, 4047.

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STAT1 Protein-DNA Interaction Assay

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IRDye labeled (5' end) sense and anti-sense GAS (sense sequence: 5'-GATGTATTTCCCAGAAAAGG-3' found upstream of the Fc-Gamma Receptor gene, 47 ) and ISRE (sense sequence: 5'-GGGAAAGGGAAACCGAAACTGAAGCC-3', found upstream of the ISG15 gene, 48 ) DNA oligonucleotides were synthesized and annealed by Integrated DNA Technologies. DNA probes (0.05 pmole dsDNA per 20 µl reaction volume) were combined ± 60 pmole STAT1 dimer in a total 20 μl reaction volume (EMSA buffer: 10 mM Tris pH 7.5, 50 mM KCl, 10 mM DTT, 0.125% Tween-20, 0.05 µg poly (dI•dC), 0.025 µg salmon sperm DNA) for 10 min at room temperature. Purified TgIST proteins were added to STAT1-DNA complexes and incubated for 10 min. Samples were mixed with LICOR 10X orange dye, resolved by 5% TBE gel (Bio-Rad), and visualized using an Odyssey infrared imager (LI-COR Biosciences).

Huang Z., Liu H., Nix J.C., Knoverek C.R., Bowman G.R., Amarasinghe G.K., & Sibley L.D. (2021). Intrinsically disordered pathogen effector alters the STAT1 dimer to prevent recruitment of co-transcriptional activators CBP/p300.

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