Androsterone

Manufactured by Steraloids

Sourced in United States

Androsterone is a naturally occurring steroid compound. It is a metabolite of the hormone testosterone. Androsterone serves as a building block for the synthesis of other important biological molecules.

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Lab products found in correlation

Progesterone, by Steraloids (1 mentions) Androstenedione, by Steraloids (1 mentions) Acetone, by Thermo Fisher Scientific (1 mentions) Ammonium hydroxide, by Thermo Fisher Scientific (1 mentions) Isopropanol, by Thermo Fisher Scientific (1 mentions) Nicotinamide adenine dinucleotide nad, by Roche (1 mentions) Nicotinamide adenine dinucleotide phosphate nadp, by Roche (1 mentions) Rotenone, by Merck Group (1 mentions) 1 acenaphthenol, by Merck Group (1 mentions) Etiocholanolone, by Steraloids (1 mentions) Dehydroepiandrosterone, by Steraloids (1 mentions) Epitestosterone, by Steraloids (1 mentions) Testosterone, by Steraloids (1 mentions) Testosterone d3, by Steraloids (1 mentions)

4 protocols using androsterone

Quantitative Steroid Profiling by LC-MS/MS

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Each sample was directly assayed; the following extraction procedure was applied to each specimen. Each sample aliquot (300 μl) was placed in a 2.0 ml autosampler vial and spiked with 150 μl of internal standard solution, i.e., androsteneione-d7 and testosterone-d3. Detection and quantitation of all analytes was accomplished using selective reaction monitoring (SRM).
Androstenedione, androsterone, progesterone and the deuterated derivative of androsteneione-d7 were obtained from Steraloids (Newport, RI), whereas testosterone-d3 was obtained from Cerillient (Round Rock, TX). Acetonitrile and methanol were HPLC grade and obtained from Burdick and Jackson (Muskesgon, MI). Acetone, isopropanol, and ammonium hydroxide were Optima grade and obtained from Fisher (St. Louis, MO). Formic acid was ACS grade and obtained from EMD (Gibbstown, NJ).

Ortega I., Villanueva J.A., Wong D.H., Cress A.B., Sokalska A., Stanley S.D, & Duleba A.J. (2014). Resveratrol potentiates effects of simvastatin on inhibition of rat ovarian theca-interstitial cells steroidogenesis. Journal of Ovarian Research, 7, 21.

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Synthesis and Characterization of Nitro-Aromatic Compounds

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3-NBA, N-OH-3-ABA, and 3-ABA were synthesized as
described previously.^{31 (link),57 (link),58 (link)} The synthesis of 3-NOBA was performed from 3-ABA
following the experimental protocol reported by Fifer et al. for the synthesis
of similar nitroso-PAHs.^{59 (link)} The
purity and identity of these compounds were verified by UV spectroscopy, high
resolution mass spectrometry, and high-field ^{1 (link)} H NMR spectroscopy. All other chemicals were of the
highest grade available, and all solvents were of HPLC grade.
(S)-(+)-1,2,3,4-Tetrahydro-1-naphthol
(S-tetralol), flavin adenine dinucleotide (FAD), dithiothreitol
(DTT), 1-acenaphthenol, dicoumarol (Dic), allopurinol, rotenone, and
D-glucose-6-phosphate (G6P) were purchased from Millipore-Sigma (St. Louis, MO).
Nicotinamide adenine dinucleotide (NAD⁺) and nicotinamide adenine
dinucleotide phosphate (NADP⁺) were purchased from Roche Diagnostics
(Indianapolis, IN). Glucose-6-phosphate dehydrogenase (G6PD) was purchased from
Worthington Biochemical Corporation (Lakewood, NJ). Androsterone was purchased
from Steraloids (Wilton, NH); 2,6-dichloroindophenol (DCPIP) and salicylic acid
were purchased from Acros Organics (Geel, Belgium). Flufenamic acid (FA),
ursodeoxycholate, and indomethacin were purchased from ICN Biomedicals, Inc.
(Aurora, Ohio).

Murray J.R., Mesaros C.A., Arlt V.M., Seidel A., Blair I.A, & Penning T.M. (2018). Role of Human Aldo-Keto Reductases in the Metabolic Activation of the Carcinogenic Air Pollutant 3-Nitrobenzanthrone. Chemical research in toxicology, 31(11), 1277-1288.

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Validation of Steroid Hormone Assay

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The EIA kit for T (Cayman Chemical Company, MI, USA) was applied according to the manufacturer’s instructions. 5α-dihydrotestosterone (DHT) and 5β-dihydrotestosterone, androstenediol, 5α-androstanedione, androsterone (ADT), eticholanolone and DHEA were purchased from Steraloids (Newport, RI, USA). All tested metabolites were dissolved in ethanol, diluted in the buffer provided with the kit and measured at three different concentrations (150 pg/mL, 75 pg/mL and 37.5 pg/mL) in duplicates. Cross-reactivity was calculated in percentage of the recovery rate of the corresponding metabolite.

Engeli R.T., Fürstenberger C., Kratschmar D.V, & Odermatt A. (2018). Currently available murine Leydig cell lines can be applied to study early steps of steroidogenesis but not testosterone synthesis. Heliyon, 4(2), e00527.

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Steroid Quantification Protocol

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Testosterone, epiTestosterone, androsterone, etiocholanolone, 5α-androstane-3α,17β-diol, 5βandrostane-3α,17β-diol, dehydroepiandrosterone, 5β-androstanedione, 4-androstenedione and Testosterone-d3 were purchased from Steraloids Inc. (Newport, RI, USA); 5α-dihydroTestosterone was provided by LGC Promochem SRL (Milan, Italy). Isopropyl alcohol, methanol, methyl tertbutyl ether (TBME), ethyl acetate, 17α-methylTestosterone, dithioerythritol and N-Methyl-N-

Alladio E., Caruso R., Gerace E., Amante E., Salomone A, & Vincenti M. (2016). Application of multivariate statistics to the Steroidal Module of the Athlete Biological Passport: A proof of concept study. Analytica chimica acta, 922.

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