Pe cy 7 anti ccr7
PE)-Cy™7-anti-CCR7 is a fluorochrome-conjugated antibody that binds to the CCR7 receptor. It is used in flow cytometry applications to identify and analyze cells expressing the CCR7 protein.
Lab products found in correlation
3 protocols using pe cy 7 anti ccr7
Quantification of Circulating Memory Tfh Cells
Characterization of Memory T Follicular Helper Cells
Multicolor Flow Cytometry of T-cell Subsets
Intracellular staining was carried out using the FoxP3 permeabilization solution kit according to the manufacturer's instructions (eBioscience, Paris, France) together with PE Ki67, PE anti-Bcl-6 (BD Pharmingen) or PE anti-FoxP3 (Biolegend), and Alexa-Fluor 488 anti-SAMHD1 (a gift from O. Schwartz) or rabbit anti-SAMHD1 (Euromedex, Souffelweyersheim, France) followed by Alexa-Fluor 647 antirabbit antibody (Invitrogen, Life Technologies, Saint Auben, France).
Dead cells were excluded using the Live/Death Vivid detection kit labeled with an aqua dye (Invitrogen). Fluorescence intensities were measured with an LSR II flow cytometer (Becton Dickinson) and analyzed using FlowJo version 7.6.5 (Tree Star Inc., Ashland, Oregon, USA).
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