Log In Sign up
The largest database of trusted experimental protocols

Human iga elisa kit

Manufactured by Abcam
Visit Supplier
Sourced in United States

The Human IgA ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of human immunoglobulin A (IgA) levels in various biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Human tslp elisa max deluxe sets, by BioLegend (2 mentions) Quantikine elisa kit, by R&D Systems (1 mentions) Micro mixer, by TAITEC (1 mentions) Anti mouse iga hrp antibody, by Southern Biotech (1 mentions)

Close spelling variants of this product

ELISAs

8 protocols using human iga elisa kit

1

Quantifying TSLP and IgA Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols
Serum levels of TSLP and IgA were measured using Human TSLP ELISA MAX Deluxe Sets (Biolegend, San Diego, CA, USA) and an IgA Human ELISA Kit (ab137980; Abcam) according to the manufacturers’ protocols. The absorbance was read at 450 and 550 nm using a Varioskan Flash 2.4 System (Thermo Fisher scientific, Waltham, MA, USA). The sensitivity of the assay was 2 pg/mL for TSLP and 1.5 ng/mL for IgA.
Li H., Kong D., Xu Y., Li X., Yao G., Chen K., You Q., Shi Q., Zhang L., Wang X., Yuan D., Miao S., Geng J., Jin X, & Meng H. (2017). Tripterygium Wilfordii inhibits tonsillar IgA production by downregulating IgA class switching in IgA nephropathy. Oncotarget, 8(65), 109027-109042.
+ Open protocol
+ Expand
2

Optimizing IgA Production in FDC Clusters

Check if the same lab product or an alternative is used in the 5 most similar protocols
For IgA production analysis in FDC-associated clusters, the base cell culture media was supplemented with TW at 1 μg/mL, 5 μg/mL, or 10 μg/mL for the initial 7 days of culture. After collection of supernatants, qualitative detection of TSLP and IgA in cell supernatants was performed using Human TSLP ELISA MAX Deluxe Sets (Biolegend, San Diego, CA, USA) and IgA Human ELISA Kit (ab137980; Abcam).
Li H., Kong D., Xu Y., Li X., Yao G., Chen K., You Q., Shi Q., Zhang L., Wang X., Yuan D., Miao S., Geng J., Jin X, & Meng H. (2017). Tripterygium Wilfordii inhibits tonsillar IgA production by downregulating IgA class switching in IgA nephropathy. Oncotarget, 8(65), 109027-109042.
+ Open protocol
+ Expand
3

Cytokine and IgA Quantification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
IL-8 and IL-10 were assayed using R&D Systems Quantikine ELISA kits (Minneapolis, MN, USA) as per manufacturer’s instructions. Results for IL-8 and IL-10 are expressed as picograms per milliliter (pg/mL). Total IgA was measured using the Abcam human IgA ELISA kit (Cambridge, UK) as per manufacturer’s instructions. Results for IgA are expressed as micrograms per milliliter (µg/mL).
Briefly, all samples were assayed in duplicate. Samples were thawed completely and vortexed thoroughly before being assayed. Controls and their two pasteurized samples were always run simultaneously on the same ELISA plate. Samples were diluted 1:20,000 for the IgA assays, while no dilution of samples was necessary for the cytokine assays.
Daniels B., Schmidt S., King T., Israel-Ballard K., Amundson Mansen K, & Coutsoudis A. (2017). The Effect of Simulated Flash-Heat Pasteurization on Immune Components of Human Milk. Nutrients, 9(2), 178.
+ Open protocol
+ Expand
4

Quantification of Fecal Immunoglobulin A

Check if the same lab product or an alternative is used in the 5 most similar protocols
Freeze-dried fecal samples were disrupted by vigorous shaking as stated previously. Ten milligrams (±0.5 mg) of the disrupted samples were mixed with 1 ml of 1 x PBS. The mixtures were homogenized by a micro mixer (TAITEC) at maximum speed for 5 min, followed by centrifugation at 10,000 × g for 10 min. Approximately 800 μl of supernatant was filtered through 0.22 μm syringe filters (Merck, SLGVR33RB) and stored in new 1.5 ml tubes. The amount of intestinal IgA from the filtered supernatant was measured by ELISA using a commercially available quantitative ELISA (Human IgA ELISA Kit, Abcam ab196263). The fecal samples were further diluted by Sample Diluent NS included in the ELISA kit, and the IgA concentration was measured according to the manufacturer’s manual.
Nishimoto Y., Kawai J., Mori K., Hartanto T., Komatsu K., Kudo T, & Fukuda S. (2023). Dietary supplement of mushrooms promotes SCFA production and moderately associates with IgA production: A pilot clinical study. Frontiers in Nutrition, 9, 1078060.
+ Open protocol
+ Expand
5

ELISA Quantification of IgA Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols
The quantitative measurement of human IgA antibody against S1 RBD protein in the 500 times diluted serum was accomplished using the COVID-19 S-Protein (S1RBD) Human IgA ELISA Kit (ab276185, Abcam). In brief: antibodies present in the sample are bound with wells covered with S1 RBD proteins. Next, secondary anti-IgA antibodies are added. Following washing, HRP-streptavidin addition, a TBM substrate was added and an enzymatic color reaction was measured at 450 nm on the spectrophotometer (Epoch, BioTek). Results were generated based on a four-parameter logistic (4PL) curve. A positive result (unit/ml) was considered when greater than 21.4 units/ml.
Piotrowska M., Zieliński M., Tylicki L., Biedunkiewicz B., Kubanek A., Ślizień Z., Polewska K., Tylicki P., Muchlado M., Sakowska J., Renke M., Sudoł A., Dąbrowska M., Lichodziejewska-Niemierko M., Smiatacz T., Dębska-Ślizień A, & Trzonkowski P. (2022). Local and Systemic Immunity Are Impaired in End-Stage-Renal-Disease Patients Treated With Hemodialysis, Peritoneal Dialysis and Kidney Transplant Recipients Immunized With BNT162b2 Pfizer-BioNTech SARS-CoV-2 Vaccine. Frontiers in Immunology, 13, 832924.
+ Open protocol
+ Expand
6

Quantifying IgA-Bound Gut Bacteria

Check if the same lab product or an alternative is used in the 5 most similar protocols
For human IgA, total IgA-bound bacteria (low- and high-affinity IgA) were quantified by ELISA. One million isolated fecal bacteria per individual were coated in duplicates and probed for IgA using a commercially available quantitative ELISA Assay (Human IgA ELISA Kit, Abcam). For mouse IgA, 100–200μl of mouse blood was collected and serum was isolated using Microvette CV 300 Z serum separation tubes (SARSTEDT Germany) according to the manufacturer’s protocol. Serum was stored at −80°C. Fecal pellets were collected, weighed and 500μl of sterile PBS was added. Pellets were vortexed on max speed at 4°C for 10min. The dissociated pellet was spun at 12000rpm for 10min and the fecal water supernatant was collected. Fecal water was stored at −80°C. Immunoglobulins were absorbed on high-bound Nunc MaxiSorp ELISA plates (BioLegend) and anti-IgA ELISA was performed using anti-mouse IgA-HRP antibody (Southern Biotech).
Rojas O.L., Pröbstel A.K., Porfilio E.A., Wang A.A., Charabati M., Sun T., Lee D.S., Galicia G., Ramaglia V., Ward L.A., Leung L.Y., Najafi G., Khaleghi K., Garcillán B., Li A., Besla R., Naouar I., Cao E.Y., Chiaranunt P., Burrows K., Robinson H.G., Allanach J.R., Yam J., Luck H., Campbell D.J., Allman D., Brooks D.G., Tomura M., Baumann R., Zamvil S.S., Bar-Or A., Horwitz M.S., Winer D.A., Mortha A., Mackay F., Prat A., Osborne L.C., Robbins C., Baranzini S.E, & Gommerman J.L. (2019). Recirculating Intestinal IgA-Producing Cells Regulate Neuroinflammation via IL-10. Cell, 176(3), 610-624.e18.
+ Open protocol
+ Expand
7

Quantifying Fecal IgA Levels

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
IgA Extraction of fecal IgA was performed as previously reported (20 (link)). Quantification of IgA amount in fecal samples was performed by ELISA using commercially available kits according to the manufacturer’s protocols (Human IgA ELISA Kit, Abcam ab196263).
Yamauchi Y., Masutomi H., Ishihara K., Hartanto T., Lee C.G, & Fukuda S. (2024). The differential effect of two cereal foods on gut environment: a randomized, controlled, double-blind, parallel-group study. Frontiers in Nutrition, 10, 1254712.
+ Open protocol
+ Expand
8

Quantifying IgA and IgG in Serum and CSF

Check if the same lab product or an alternative is used in the 5 most similar protocols
IgA in serum and CSF was quantified using a commercially available quantitative ELISA assay (Human IgA ELISA Kit, Abcam). IgG was measured as part of routine clinical testing with a nephelometric assay. Intrathecal immunoglobulin synthesis (Ig index) was calculated by correcting for albumin.
Pröbstel A.K., Zhou X., Baumann R., Wischnewski S., Kutza M., Rojas O.L., Sellrie K., Bischof A., Kim K., Ramesh A., Dandekar R., Greenfield A.L., Schubert R.D., Bisanz J.E., Vistnes S., Khaleghi K., Landefeld J., Kirkish G., Liesche-Starnecker F., Ramaglia V., Singh S., Tran E.B., Barba P., Zorn K., Oechtering J., Forsberg K., Shiow L.R., Henry R.G., Graves J., Cree B.A., Hauser S.L., Kuhle J., Gelfand J.M., Andersen P.M., Schlegel J., Turnbaugh P.J., Seeberger P.H., Gommerman J.L., Wilson M.R., Schirmer L, & Baranzini S.E. (2020). Gut microbiota-specific IgA+ B cells traffic to the CNS in active multiple sclerosis. Science immunology, 5(53), eabc7191.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!