Cell culture plates and flasks (T-75 mm) were obtained from Corning Incorporated (Corning, NY, USA); Kaighn’s modification of Ham’s F-12K medium was purchased from ATCC (Manassas, VA, USA). Fetal bovine serum (FBS), and penicillin/streptomycin antibiotic solution were both purchased from Sigma-Aldrich, Inc. (St. Louis, MO, USA). PBS, trypsin, trypsin neutralizing solution (TNS), and trypan blue cell viability dye were purchased from Lonza Inc. (Walkersville, MD, USA). The 0.45 and 0.22 µm filter units (with syringe) were purchased from Millipore (Carrigtwohill, Co. Cork, Ireland). The lipid peroxidation kit was purchased from AbCam. The [3-(4,5-dimethyl-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] (MTS assay kit was purchased from Promega Corp (specifically the CellTiter 96 AQueous One Solution Cell Proliferation Assay; Madison, WI, USA). The comet assay and annexin V assay kits were both purchased from Trevigen Inc. (Gaithersburg, MD, USA).
Trypan blue cell viability dye
Manufactured by Lonza
Sourced in United States
Trypan blue is a dye used to assess cell viability. It is a basic dye that selectively stains dead cells blue, allowing them to be easily distinguished from viable cells under a microscope.
Automatically generated - may contain errors
Lab products found in correlation
Lipid peroxidation kit, by Abcam (2 mentions)
Cell culture plates and flasks t 75 mm, by Corning (2 mentions)
Kaighn s modification of ham s f 12k medium, by American Type Culture Collection (2 mentions)
3 4 5 dimethyl 2 yl 5 3 carboxymethoxyphenyl 2 4 sulfophenyl 2h tetrazolium mts assay kit, by Promega (2 mentions)
Celltiter 96 aqueous one solution cell proliferation assay, by Promega (2 mentions)
Fetal bovine serum (fbs), by Merck Group (2 mentions)
Phosphate buffered saline (pbs), by Lonza (2 mentions)
Trypsin neutralizing solution, by Lonza (2 mentions)
Penicillin streptomycin antibiotic solution, by Merck Group (2 mentions)
Trypsin, by Lonza (2 mentions)
2 protocols using trypan blue cell viability dye
1
Comprehensive Cell Culture Workflow
2
Cell Culture and Assay Protocols
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