MAGI-CCR5 was obtained through the NIH AIDS Research Reagent Program (cat. no. 3522) from Julie Overbaugh [31] (link). The human HEK293T (ATCC, cat. no. CRL-11268) and MAGI-CCR5 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen) with 10% fetal bovine serum and penicillin/streptomycin (D-10 medium) and passaged upon confluency. DNA transfection was carried out using polyethylenimine (PEI) reagent according to the manufacturer’s instructions (Polyscience, cat. no. 23966-2). The following antibodies or sera were used in this study: β-tubulin monoclonal antibody (mAb; Covance, cat. no. NMS-410P), anti-CUL5 (H-300) rabbit polyclonal antibody (Santa Cruz Biotechnology, cat. no. sc-13014), anti-ElonginB (FL-118) rabbit polyclonal antibody (Santa Cruz Biotechnology, cat. no. sc-1144), anti-V5 mAb (Invitrogen, cat. no. R960-25), anti-CBFβ mAb (Santa Cruz Biotechnology, cat. no. sc-166142), CAp24 mAb (NIH AIDS Reagents Program, cat no. 1513) and anti-HA rabbit polyclonal antibody (Santa Cruz Biotechnology, cat. no. 71–5500).
Anti elonginb fl 118 rabbit polyclonal antibody
Manufactured by Santa Cruz Biotechnology
Anti-ElonginB (FL-118) rabbit polyclonal antibody is a laboratory reagent used for the detection and quantification of ElonginB protein. It is a specific antibody raised in rabbits against the full-length ElonginB protein.
Automatically generated - may contain errors
Lab products found in correlation
Anti cul5 h 300 rabbit polyclonal antibody, by Santa Cruz Biotechnology (2 mentions)
Anti ha rabbit polyclonal antibody, by Santa Cruz Biotechnology (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Anti v5 mab, by Thermo Fisher Scientific (1 mentions)
Monoclonal anti v5 antibody, by Thermo Fisher Scientific (1 mentions)
2 protocols using anti elonginb fl 118 rabbit polyclonal antibody
1
MAGI-CCR5 Cell Line Maintenance and Transfection
2
HEK293T and MAGI Cell Maintenance and Transfection
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The human HEK293T (ATCC, catalog no. CRL-11268) and MAGI (NIH-ARRRP, catalog no. 3522) cells from Julie Overbaugh [53 (link)] were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen) with 10% fetal bovine serum and penicillin/streptomycin (D-10 medium) and passaged upon confluence. DNA transfection with PEI reagent was carried out according to the manufacturer’s instructions (Polyscience, catalog no. 23966-2). The following antibodies or antisera were used in this study: anti-CUL5 (H-300) rabbit polyclonal antibody (Santa Cruz Biotechnology, sc-13014), anti-Vif rabbit polyclonal antibody (NIH-ARRRP, catalog no 2221), CAp24 monoclonal antibody (NIH-ARRRP, catalog no. 1513), anti-HA rabbit polyclonal antibody (Santa Cruz Biotechnology, 71-5500), anti-CBF-β monoclonal antibody (Santa Cruz Biotechnology, sc-166142), anti-ElonginB (FL-118) rabbit polyclonal antibody (Santa Cruz Biotechnology, sc-1144), anti-V5 monoclonal antibody (Invitrogen, R960-25), anti-β-tubulin monoclonal antibody (Tianjin Sanjian, catalog no. DKM9003). MG132 was purchased from Sigma (catalog no. C2211).
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