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Bioultra 8000

Manufactured by Merck Group
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The BioUltra 8000 is a high-performance laboratory centrifuge designed for a wide range of applications in the life sciences and biomedical research. It features a powerful motor, precise speed control, and a robust construction for reliable operation.

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Lab products found in correlation

Ammonium nitrate, by Thermo Fisher Scientific (1 mentions) Thioglycolic acid, by Merck Group (1 mentions) Cadmium acetate dihydrate, by Merck Group (1 mentions) Sodium ascorbate, by Merck Group (1 mentions) Magnesium sulfate, by Merck Group (1 mentions) Sodium hydroxide (naoh), by ITW Reagents (1 mentions) P toluenesulfonyl chloride, by Merck Group (1 mentions) 3 aminopropyltriethoxysilane, by Merck Group (1 mentions) Cytochrome c cyt, by Merck Group (1 mentions) Sodium tellurite, by Thermo Fisher Scientific (1 mentions) Hydrochloric acid (hcl), by Merck Group (1 mentions) Tetraethyl orthosilicate, by Merck Group (1 mentions) Chloroform, by Carlo Erba (1 mentions) Multimode optical fiber, by Thorlabs (1 mentions) Phosphate buffered saline (pbs), by Quality Biological (1 mentions) Methanol, by Thermo Fisher Scientific (1 mentions)

3 protocols using bioultra 8000

1

Synthesis and Characterization of Nanoparticles

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Thioglycolic acid (TGA, C2H4O2S, ≥99%), cadmium acetate dihydrate (Cd(CH3COO)2·2H2O, 99.5%), (3-aminopropyl)triethoxysilane (APTES, H2N(CH2)3Si(OC2H5)3, 99%), tetraethyl orthosilicate (TEOS, SiC8H20O4, 98%), ammonia (NH3, ≥99.9%), cetyltrimethylammonium bromide (CTAB, C19H42BrN, ≥98%), poly(ethylene glycol) BioUltra 3000, poly(ethylene glycol) BioUltra 6000, poly(ethylene glycol) BioUltra 8000, p-toluenesulfonyl chloride (CH3C6H4SO2Cl, ≥98%), hydrochloric acid (HCl, 37%), magnesium sulfate (MgSO4, ≥99.5%), sodium ascorbate, bovine serum albumin (BSA), lysozyme (LYS), carbonic anhydrase (CA), ovalbumin (OVA), hemoglobin (Hb), myoglobin (Myb), and cytochrome c (CYT) were purchased from Sigma-Aldrich (Lisbon, Portugal). Sodium hydroxide (NaOH, ≥98%) was produced by Panreac. Sodium tellurite (Na2TeO3, 99.5%) and ammonium nitrate (NH4NO3, 99.9%) were purchased from Alfa Aesar. Sodium borohydride (NaBH4, 99%), ethylene glycol (C2H6O2, ≥99.5%) and (3-isocyanatopropyl)triethoxysilane (IPTES, (C2H5O)3Si(CH2)3NCO, 95%) were produced by Fluka. Methanol (MeOH), ethanol (EtOH), tetrahydrofuran (THF, C4H8O, ≥99.9%), and chloroform (CHCl3) were purchased by Carlo Erba Reagents. All the reagents and solvents were of analytical reagent grade and were used as received. All cell culture reagents were purchased from Gibco (Alfagene, Lisbon, Portugal) unless stated otherwise.
Marcelo G., Ariana-Machado J., Enea M., Carmo H., Rodríguez-González B., Luis Capelo J., Lodeiro C, & Oliveira E. (2018). Toxicological Evaluation of Luminescent Silica Nanoparticles as New Drug Nanocarriers in Different Cancer Cell Lines. Materials, 11(8), 1310.
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2

Tetrode Electro-Plating for Neural Recording

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After gluing the tetrodes to the guide structure and physically connecting each tetrode wire to the electrode vias in the EIB, tetrodes' tips were cut with sharp stainless-steel scissors (14568-09, Fine Science Tools) and electroplated. The plating solution consisted of 5% gold non-cyanide solution (Sifco 5355 Gold Plating Solution, Neuralynx) with 1% Poly(ethylene glycol) (PEG) addictive (BioUltra, 8,000, Sigma-Aldrich) mixed in a 75% v/v PEG to gold ratio. This solution has been shown to promote a large surface area with low impedance [10] (link). Electrode impedance was lowered to 150-200 KOhm in successive steps of electrodeposition at -0.05 µA with the aid of NanoZ (White Matter LLC).
Machado F., Sousa N., Monteiro P, & Jacinto L. (2020). A versatile and modular tetrode-based device for single-unit recordings in rodent ex vivo and in vivo acute preparations. Journal of neuroscience methods, 341.
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3

Optical Fiber-Based Heparin Sensing

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Nile blue A and polyethylene glycol (Mn 7,000–9,000) (Bioultra 8000) were purchased from Sigma Aldrich Inc. and used as received. Phosphate buffered saline was purchased from Quality Biological and used without further purification. Methanol was purchased from Fisher and used without further purification. Whatman No. 2 filter paper circles were purchased from GE Healthcare, U.K. A multi-mode optical fiber with core diameter of 400 μm were purchased from Thorlabs, USA. Miniature Heat-Shrink Tubing was purchased from McMaster-Carr, USA. Transpore tapes were purchased from 3M. Unfractionated heparin (1000 U/ml) was purchased from McKesson Corporation, USA. Polytetrafluoroethylene (PTFE) tubing was purchased from Agilent Technologies.
Zhou J., Yim W., Zhou J., Jin Z., Xu M., Mantri Y., He T., Cheng Y., Fu L., Wu Z., Hancock T., Penny W, & Jokerst J.V. (2021). A fiber optic photoacoustic sensor for real-time heparin monitoring. Biosensors & bioelectronics, 196, 113692.
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