Ckx41 optical microscope

Manufactured by Olympus

Sourced in Japan

The CKX41 optical microscope is a high-quality, versatile instrument designed for various laboratory applications. It features a sturdy metal frame, a Brightfield illumination system, and a range of magnification levels from 40x to 400x. The CKX41 is equipped with a professional-grade optics system, ensuring clear, high-resolution images for precise observation and analysis.

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Lab products found in correlation

Quantum supporting xcapture software, by PerkinElmer (2 mentions) Quantum gx microct imaging system, by PerkinElmer (2 mentions) Masson trichrome, by Solarbio (2 mentions) Hematoxylin and eosin staining kit, by Beyotime (1 mentions) Transwell chamber, by Corning (1 mentions) Crystal violet solution, by Sangon (1 mentions) Ab85051, by Abcam (1 mentions) Ab64238, by Abcam (1 mentions) Ab205718, by Abcam (1 mentions) Microtome, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

CKX41 (1569 citations) Optical microscope (1271 citations) CKX41 microscope (390 citations) CKX41 inverted optical microscope (2 citations)

9 protocols using ckx41 optical microscope

Histopathological Evaluation of Organ Toxicity

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Healthy
rabbits were
used as the control group. The livers, heads, and kidneys of all animals
were pathologically examined shortly after they were sacrificed. The
samples were fixed in 10% (w/v) formalin (Beijing Yili, China) and
embedded in paraffin. Each specimen was sectioned (5 μm thick),
and deparaffinized sections were stained with the hematoxylin–eosin
(HE) method. Then, the samples were visualized and photographs were
taken through an Olympus CKX41 optical microscope (Japan) to evaluate
systemic toxicity.

Xi Z., Wu Y., Xiang S., Sun C., Wang Y., Yu H., Fu Y., Wang X., Yan J., Zhao D., Wang Y, & Zhang N. (2020). Corrosion Resistance and Biocompatibility Assessment of a Biodegradable Hydrothermal-Coated Mg–Zn–Ca Alloy: An in Vitro and in Vivo Study. ACS Omega, 5(9), 4548-4557.

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Osteogenic Differentiation of hUC-MSCs

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This study was approved by the Animal Ethical and Welfare Committee of Central South University. The hUC‐MSCs transfected with pCDH‐linc02349 or pCDH‐control vector were induced in osteogenic medium at 37°C for 7 days. Then, the cells were harvested, and 2 × 10⁶ cells were incubated with Bio‐Oss Collagen scaffolds (5 × 5 × 1.75 mm³) (Geistlich) scaffolds for 1 hour, which were transplanted subcutaneously in right back of BALB/c nude mice(5 weeks old, male, n = 5 per group). After 8 weeks, the Bio‐Oss Collagen were obtained and fixed in 4% paraformaldehyde. The implants were analysed via Quantum GX micro‐CT Imaging System (PerkinElmer), with the Quantum Supporting Xcapture Software (PerkinElmer) used to analyse the BV/TV. After that, implants were decalcified in 10% EDTA for 14 days, embedded with paraffin and sliced into sections (4 μm thickness). The sections were stained with H&E (Boster) and Masson Trichrome (Solarbio). In addition, IHC staining was performed with anti‐OPN. The images were taken using CKX41 optical microscope (Olympus).

Cao L., Liu W., Zhong Y., Zhang Y., Gao D., He T., Liu Y., Zou Z., Mo Y., Peng S, & Shuai C. (2020). Linc02349 promotes osteogenesis of human umbilical cord‐derived stem cells by acting as a competing endogenous RNA for miR‐25‐3p and miR‐33b‐5p. Cell Proliferation, 53(5), e12814.

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Histological Analysis of Liver Tissues

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Liver tissues were removed immediately and fixed in 10% neutral formalin solution for at least 24 h, then embedded in paraffin wax and sectioned (4 µm thickness) for histopathological evaluation. Liver sections were stained with hematoxylin and eosin (H&E) using the Hematoxylin and Eosin Staining kit (C0105; Beyotime Institute of Biotechnology, Haimen, China), then the sections were analyzed by light microscopy (CKX41 Optical Microscope; Olympus).

CHEN L.Y., YANG B., ZHOU L., REN F., DUAN Z.P, & MA Y.J. (2015). Promotion of mitochondrial energy metabolism during hepatocyte apoptosis in a rat model of acute liver failure. Molecular Medicine Reports, 12(4), 5035-5041.

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Evaluating Bone Regeneration in Mice

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After 8 weeks of osteogenic differentiation in vivo, all nude mice were sacrificed, and the Bio-Oss Collagen was stripped from the subcutaneous skin and fixed in 4% PFA. The specimens were analyzed using Quantum GX microCT Imaging System (PerkinElmer, CA, USA). BV/TV and BMD were measured using Quantum Supporting Xcapture Software (PerkinElmer, CA, USA). Then the specimens were decalcified in 10% EDTA solution for 14 days, followed by embedding with paraffin and sectioning. The sections (4.0 μm) were stained with H and E (Boster, USA) and Masson Trichrome (Solarbio, Beijing, China). In addition, IHC staining was performed with anti-OCN and anti-OSX to evaluate the expression of osteogenic markers. The images were taken using CKX41 Optical microscope (Olympus, Japan).

He S., Yang S., Zhang Y., Li X., Gao D., Zhong Y., Cao L., Ma H., Liu Y., Li G., Peng S, & Shuai C. (2019). LncRNA ODIR1 inhibits osteogenic differentiation of hUC-MSCs through the FBXO25/H2BK120ub/H3K4me3/OSX axis. Cell Death & Disease, 10(12), 947.

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Transwell Invasion Assay for miR-29c and HBP1

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HK1, HNE1, and CNE2 cells were transfected with miR-29c mimics or siHBP1 as described above. 5.0 × 10⁴ cells were resuspended in 200 μl 1640 medium without FBS and seeded into matrigel-covered Transwell Chamber (Costar, USA) (upper chamber), meanwhile, 700 μl 1640 medium containing 15% FBS was added to the lower chamber. We terminated the culture when cells were invaded through the membrane and migrate into the lower side of the chamber membrane, then the cells which invaded into the lower side were fixed and dyed with crystal violet solution (0.1%, Sangon Biotech Co, Ltd, Shanghai, China) for 5 min, the cells on the upper side were wiped off using cotton swabs and the lower side of the membrane was washed with appropriate PBS solution and photographs were taken under CKX41 optical microscope (Olympus, Japan). Photographs with five fields of every group were taken and the cells were counted using Image-Pro Plus software.

He S., Yang S., Niu M., Zhong Y., Dan G.a.o., Zhang Y., Ma H., Xiong W., Zhou M., Zhou Y., Xiang B., Li G., Shuai C, & Peng S. (2018). HMG-box transcription factor 1: a positive regulator of the G1/S transition through the Cyclin-CDK-CDKI molecular network in nasopharyngeal carcinoma. Cell Death & Disease, 9(2), 100.

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Immunohistochemical Staining of BTG2

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Tissue samples were sectioned into 5 μm-thick slices, deparaffinized in xylene, and rehydrated in a series of graded alcohols. Antigen retrieval was performed by immersing the slides in sodium citrate. Endogenous peroxidase was blocked by a 10-min incubation with 3% H
₂O
₂. Next, the slices were incubated with primary antibody anti-BTG2 (ab85051; Abcam, Cambridge, UK) overnight at 4°C, washed three times with PBS, and incubated with horseradish peroxidase (HRP)-conjugated secondary antibody (ab205718; Abcam) for 30 min. Finally, immunostaining was performed using a diaminobenzidine (DAB) substrate kit (ab64238; Abcam). The sections were observed with a CKX41 optical microscope (Olympus, Tokyo, Japan).

Huang H., Li X., Zhang X., Li Z., Han D., Gao W., Liu L., Peng C., Zhu H, & Yu X. (2022). DSCR9/miR-21-5p axis inhibits pancreatic cancer proliferation and resistance to gemcitabine via BTG2 signaling: DSCR9/miR-21-5p/BTG2 regulates pancreatic cancer. Acta Biochimica et Biophysica Sinica, 54(12), 1775-1788.

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Immunohistochemical Analysis of ClC-5 in Osteosarcoma

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Immunohistochemistry for ClC-5 was performed on 4-μm paraffin-embedded sections from human osteosarcoma tissues using the streptavidin-biotin-peroxidase complex system according to the supplier’s instructions (DAKO Japan, Tokyo, Japan). The sections were heated for 30 min at 65°C, dewaxed in xylene, and rehydrated by 100, 95, 70, and 50% alcohols at room temperature for 1 min. The sections were blocked by 10% goat serum and incubated with ClC-5 antibody overnight at 4°C, followed by incubation with biotinylated secondary antibody. The expression of ClC-5 was visualized with the streptavidin-peroxidase reaction using 3,3’-diaminobenzidine under CKX41 optical microscope (Olympus, Japan).

Peng F., Cai W., Li J, & Li H. (2021). ClC-5 Downregulation Induces Osteosarcoma Cell Apoptosis by Promoting Bax and tBid Complex Formation. Frontiers in Oncology, 10, 556908.

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Immunohistochemical Analysis of Tumor Tissues

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Tumor tissues were harvested from mice and fixed overnight at 4 °C in 4% PFA. After washing three times with PBS, the tissues were transferred to a 30% sucrose solution and incubated overnight at 4 °C for cryoprotection. The tissues were then mounted in optimal cutting temperature (OCT) embedding compound (Thermo Scientific, USA) and frozen at -80 °C. Tissue slices (6 μm thick) were cut using a microtome (Thermo Scientific, USA) and mounted on glass slides. The slices were stained with biotinylated anti-Ki67, anti-CD31, and anti-ASNS antibodies, and visualized using an HRP/DAB (ABC) detection IHC kit. Immunohistochemistry images were obtained under a CKX41 optical microscope (Olympus, Japan). Signal intensities were quantified using ImageJ software (NIH, USA).

Zhang Y., Akhil V., Seo H.S., Park H.R., Kim S.H., You S.H., Liu Z., Kim S.Y., Sultonova R.D., Min J.J, & Hong Y. (2024). The combination of calreticulin-targeting L-ASNase and anti-PD-L1 antibody modulates the tumor immune microenvironment to synergistically enhance the antitumor efficacy of radiotherapy. Theranostics, 14(3), 1195-1211.

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Wound Healing Assay for Cell Migration

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Wound healing assay was performed by following the protocol provided in the literature [41 (link)]. MDA-MB-231 cells were cultured in a 24-well plate and treated with siCXCR4 or siMOCK complexed with either PEI-GO or Lipofectamine 2000 for 48 h. The cell monolayer in each well was scraped with a p200 pipet tip to create a gap. After washing with culture medium to remove cell debris, the cells were allowed to migrate for 24 h, followed by observation under an Olympus CKX41 optical microscope.

Huang Y.P., Hung C.M., Hsu Y.C., Zhong C.Y., Wang W.R., Chang C.C, & Lee M.J. (2016). Suppression of Breast Cancer Cell Migration by Small Interfering RNA Delivered by Polyethylenimine-Functionalized Graphene Oxide. Nanoscale Research Letters, 11, 247.

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