Imatinib glivec

Manufactured by Novartis

Sourced in Switzerland

Imatinib (Glivec) is a tyrosine kinase inhibitor used in the treatment of certain types of cancer, including chronic myeloid leukemia and gastrointestinal stromal tumors. It functions by inhibiting the activity of specific enzymes that promote the growth and division of cancer cells.

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Lab products found in correlation

Dulbecco s modified eagle medium, by Thermo Fisher Scientific (2 mentions) Antibiotics antimycotic, by Thermo Fisher Scientific (1 mentions) X vivo 15 chemically defined serum free hematopoietic cell medium, by Lonza (1 mentions) Antibiotics antimycotics, by Biowest (1 mentions) Fetal bovine serum (fbs), by Biowest (1 mentions) L glutamine, by Lonza (1 mentions) Secondary goat antirabbit irdye 680 conjugated antibody, by LI COR (1 mentions) Tgf β1, by Abcam (1 mentions) Bay11 7082, by Merck Group (1 mentions)

3 protocols using imatinib glivec

Cultivation of Leukemic and Fibroblast Cell Lines

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The ETV6-ABL1-positive B-cell precursor leukemic cell line ALL-VG^{32 (link)} was grown in X-VIVO 15 Chemically Defined Serum-free Hematopoietic Cell Medium (Lonza, Basel, Switzerland) with 3 mM L-Glutamine (Lonza) and antibiotics/antimycotics (Thermo Fisher Scientific, Waltham, MA, USA). The established TKI-resistant ALL-VG cell line was cultivated in the same medium supplemented with 1.5 μM imatinib (Glivec; Novartis, Basel, Switzerland). The HEK293T (human embryonic kidney carcinoma) and NIH3T3 (human embryonic fibroblasts) cell lines were grown in Dulbecco’s Modified Eagle Medium (Thermo Fisher Scientific) with 10% fetal bovine serum (Biowest, Nuaillé, France) and antibiotics/antimycotics.

Zimmermannova O., Doktorova E., Stuchly J., Kanderova V., Kuzilkova D., Strnad H., Starkova J., Alberich-Jorda M., Falkenburg J.H., Trka J., Petrak J., Zuna J, & Zaliova M. (2017). An activating mutation of GNB1 is associated with resistance to tyrosine kinase inhibitors in ETV6-ABL1-positive leukemia. Oncogene, 36(43), 5985-5994.

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Antibody Characterization for Cell Signaling Analysis

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Rabbit polyclonal antibody against phospho-PDGFRβ (catalog n. sc-21902) and goat polyclonal antibody against PDGFRβ (catalog n. sc-1627) were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Rabbit monoclonal antibody (EP1255Y) against MMP9, Rabbit polyclonal antibody against NG2 (catalog num. ab129051), rabbit monoclonal antibody (E184) against α smooth muscle Actin (α-SMA, catalog n. ab32575), Rabbit polyclonal antibody against transforming growth factor beta-1 proprotein (TGF-β1, catalog n. ab66043), and rabbit monoclonal (EPR3776) against vimentin (catalog n. ab92547) were purchased from Abcam (Cambridge, UK). Rabbit polyclonal antibody against fibroblast-specific protein 1 (S100A4) (catalog n. ABF32) was purchased from Millipore (Darmstadt, Germany). Mouse monoclonal antibody (BA3R) against β–actin (catalog n. MA5-15739) was purchased from Invitrogen, Thermo Fisher Scientific (Waltham, MA, USA). Secondary goat antirabbit IRDye 680 conjugated antibody (catalog n. 926-32221) and secondary goat antimouse IRDye 800 conjugated antibody (926-32210) were purchased from LI-COR (Lincoln, NE, USA). Imatinib (Glivec) was obtained from Novartis (Basel, Switzerland). Media, antibiotics, and other reagents for cell culture were obtained from Invitrogen, Thermo Fisher Scientific.

Anfuso C.D., Longo A., Distefano A., Amorini A.M., Salmeri M., Zanghì G., Giallongo C., Giurdanella G, & Lupo G. (2020). Uveal Melanoma Cells Elicit Retinal Pericyte Phenotypical and Biochemical Changes in an in Vitro Model of Coculture. International Journal of Molecular Sciences, 21(15), 5557.

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Imatinib and BAY11-7082 Preparation

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Imatinib (Glivec), obtained from Novartis, was dissolved in DMSO, and diluted to appropriate concentrations. BAY11–7082, obtained from Sigma, was dissolved in DMSO and stored at −20°C.

Lai S., Wang G., Cao X., Luo X., Wang G., Xia X., Hu J, & Wang J. (2015). KIT over-expression by p55PIK-PI3K leads to Imatinib-resistance in patients with gastrointestinal stromal tumors. Oncotarget, 7(2), 1367-1379.

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