3T3-L1 cell (BCRC Number: 60159) was obtained from the Bioresource Collection and Research Center (BCRC, Food Industry Research and Development Institute, Hsinchu, Taiwan). Oil red O solution, 3-isobutyl-1-methylxanthine, dexamethasone, insulin, betanin, rutin and kaempferol were obtained from Sigma (St. Louis, MO, USA). The dye MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) was purchased from PanReac AppliChem (Darmstadt, Germany). Dulbecco’s modified Eagle’s medium (DMEM), Trypsin-EDTA, penicillin-streptomycin, bovine calf serum, and fetal bovine serum were purchased from Gibco (Grand Island, NY, USA). Anti-PPARγ and anti-β-actin were purchased from Cell Signaling Technology (Danvers, MA, USA). All other chemicals were reagent grade.
3t3 l1 cell
Manufactured by RIKEN BioResource Center
The 3T3-L1 cell is a fibroblast-like cell line derived from mouse embryonic 3T3 cells. The cells are capable of differentiating into adipocytes, making them a commonly used model for the study of adipogenesis and related metabolic processes.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Bovine calf serum (bcs), by Thermo Fisher Scientific (2 mentions)
Methyl thiazolyl tetrazolium (mtt), by ITW Reagents (2 mentions)
Oil red o solution, by Merck Group (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
3 isobutyl 1 methylxanthine, by Merck Group (2 mentions)
Insulin, by Merck Group (2 mentions)
Dexamethasone (dex), by Merck Group (2 mentions)
Betanin, by Merck Group (1 mentions)
Anti pparγ, by Cell Signaling Technology (1 mentions)
Anti β actin, by Cell Signaling Technology (1 mentions)
Kaempferol, by Merck Group (1 mentions)
Rutin, by Merck Group (1 mentions)
Chlorogenic acid, by Thermo Fisher Scientific (1 mentions)
Anti α actin, by Cell Signaling Technology (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Anti ppar, by Cell Signaling Technology (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
2 protocols using 3t3 l1 cell
1
3T3-L1 Cell Adipogenesis Assay
2
Adipogenesis of 3T3-L1 Cells
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3T3-L1 cell (BCRC Number: 60159) was obtained from the Bioresource Collection and Research Center (BCRC, Food Industry Research and Development Institute, Hsinchu, Taiwan). Chlorogenic acid was obtained from the Alfa Aesar (Heysham, Lancashire United Kingdom). Oil red O solution, 3-isobutyl-1-methylxanthine, dexamethasone, and insulin were obtained from Sigma (St. Louis, MO, USA). The dye MTT (3-[4, 5dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide) was purchased from PanReac AppliChem (Darmstadt, Germany). Dulbecco's modified Eagle's medium (DMEM), Trypsin-EDTA, penicillin-streptomycin, bovine calf serum, and fetal bovine serum were purchased from Gibco (Grand Island, NY, USA). Anti-PPAR and anti-αactin were purchased from Cell Signaling Technology (Danvers, MA, USA). All other chemicals were reagent grade.
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