Hcc 1187bl

The cancer cell lines (COLO-829 ATCC CRL-1974, COLO-829BL ATCC CRL-1980, HCC-1143 ATCC CRL-2321, HCC-1143 BL ATCC CRL-2362, HCC-1187 ATCC CRL-2322 and HCC-1187BL ATCC CRL-2323) were obtained from ATCC¹⁸ . The cell lines were cultured using the recommendations from ATCC. Cultured cells were split into two aliquots for metaphase chromosome preparation and karyotype analysis. Representative images and karyotypes are reported in Supplemental Fig. S1. The number of passages is indicated in Supplemental Table 1.

Original cell line stocks for HCC38, HCC1143, HCC1187, HCC1954, HCC1937 and HCC1395 were obtained directly from the American Type Culture Collection (ATCC), cultured according to Neve et al [17 (link)], and passaged minimally prior to harvesting for the present study. For each respective cell line, normal matched DNA derived from B lymphoblastoid cell lines (HCC38 BL, HCC1143 BL, HCC1187 BL, HCC1954 BL, HCC1937 BL, HCC1395 BL) were purchased from ATCC. Breast cancer cells were harvested at approximately 75% confluency and genomic DNA was isolated using DNeasy Blood and Tissue Kit (Qiagen) according to the standard manufacturer protocol. The concentration of DNA was measured with the ND-1000 NanoDrop spectrophotometer (NanoDrop Technologies).