All subsequent assays were carried out on a Beckman Coulter fluorescence-activated cell sorter (CyAn ADP 7; Brea, CA, USA). rPT cells were seeded in 6-well plates and treated with 0, 1.25, 2.5, or 5.0 μM cadmium for 12 h when the cell fusion rate was 60–70%. Subsequently, the adherent cells were collected with the Accutase cell detachment solution by 5-min centrifugation at 1500 rpm. Each treatment group yielded at least 1.5 × 106 cells, which were washed twice with PBS and incubated with fluorescent dyes for the flow cytometric analysis.
Cyan adp 7
Manufactured by Beckman Coulter
Sourced in United States
The CyAn ADP 7 is a flow cytometer designed for high-throughput analysis of cells and particles. It features seven laser excitation sources and up to 16 detection channels, enabling comprehensive multiparameter analysis.
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Lab products found in correlation
3 protocols using cyan adp 7
1
Cadmium-Induced Cell Fusion Assay
2
Evaluating Cd-induced Apoptosis and Autophagy
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rPT cells were seeded in 6-well plates and treated with Cd and/or inducer or inhibitor for 6 or 12 h when the cell fusion rate was 60–70%. Subsequently, the adherent cells were collected with Accutase™ Cell Detachment Solution by 5-min centrifugation at 1500 rpm. Each treatment group yielded 1.5 × 106 cells, which were washed twice with ice cold PBS and incubated with fluorescent dyes for the flow cytometric analysis. The levels of apoptosis and autophagy were determined using a Beckman Coulter fluorescence-activated cell sorter (CyAn ADP 7; Brea, CA, USA).
3
Annexin V-FITC Apoptosis Assay
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For apoptosis measurements, harvested cells were stained with FITC Annexin V Apoptosis Detection Kit I (BD Pharmingen, USA) according to the manufacturer’s instructions. Harvested cells were washed with cold PBS twice and resuspended in binding buffer containing annexin V-FITC and PI. Cell populations labeled with FITC and PI were analyzed by flow cytometry (CyAn ADP7, Beckman Coulter) with Summit 4.3 software (Beckman Coulter), and showed as a percentage.
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