A deposition system (Balzers Med010) was used to evaporate a homogeneous layer of carbon. The samples were collected over 400 mesh Cu grids. The grids were then negatively stained with a solution of 1% (w/w) uranyl acetate. Micrographs were recorded on an FEI Tecnai Sphera microscope operating at 200 kV and equipped with a LaB6 electron gun, using the standard cryotransfer holders developed by Gatan, Inc. For image processing, micrographs were digitized in a Zeiss SCAI scanner with different sampling windows.
Lab6 electron gun
Manufactured by Ametek
The LaB6 electron gun is a core component in electron microscope systems. It serves as the source of electron beam, providing a high-intensity, stable, and coherent electron beam for imaging and analysis applications.
Automatically generated - may contain errors
2 protocols using lab6 electron gun
1
Evaporated Carbon Layer Characterization
2
Peptide Fibrillization and Electron Microscopy
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Stock solutions
of 1 mM peptides were allowed to fibrillize in water overnight at
room temperature, diluted in phosphate-buffered saline (PBS) to 0.2
mM, and applied to 200 mesh copper grids with carbon support film
(Quantifoil). The grids were negatively stained with 2% uranyl acetate,
and imaged on a JEM1400 TEM (JEOL) equipped with LaB6 electron
gun and Ultrascan 1000 camera (Gatan).
of 1 mM peptides were allowed to fibrillize in water overnight at
room temperature, diluted in phosphate-buffered saline (PBS) to 0.2
mM, and applied to 200 mesh copper grids with carbon support film
(Quantifoil). The grids were negatively stained with 2% uranyl acetate,
and imaged on a JEM1400 TEM (JEOL) equipped with LaB6 electron
gun and Ultrascan 1000 camera (Gatan).
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