Uv 2450 2550 spectrophotometer

The amino contents of CMCS and GA-g-CMCS were determined based on a reported method with slight modification [54 (link)]. CMCS and GA-g-CMCS were separately dissolved in deionized water to a concentration of 1 mg/mL. A total of 2 mL of ninhydrin solution (50 mg/mL, ninhydrin in dimethylformamide) and 0.5 mL of acetate buffer (0.2 M, pH 5.5) were added to 0.5 mL of the sample solution. After reacting in boiling water for 30 min, the samples were cooled to room temperature. The absorbance at 574 nm was measured to compare changes in amino content of CMCS and GA-g-CMCS using a UV-2450/2550 spectrophotometer (Shimadzu, Kyoto, Japan).

UV/Visible spectra were recorded on a Shimadzu UV2450-2550 spectrophotometer (Shimadzu, Kyoto, Japan) in 1 cm cuvettes. The infrared spectrum was obtained from KBr plates using a Nicolet AVATAR 360 FTIR spectrophotometer (Nicolet, Madison, WI, USA). ¹H-NMR spectrum was recorded on a Bruker DPX 400 spectrometer (Bruker, Karlsruhe, Germany). Elemental analysis was performed on a Vario EL cube CHNs analyzer (Elementar, Frankfort, Germany). X-ray crystallography was carried out with a SMART CCD (Bruker, Karlsruhe, Germany). ESI mass spectra were recorded on a PE-SCIEX API 365 triple quadruple mass spectrometer (AB Sciex, Boston, MA, USA).