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Atp detection kit

Manufactured by Cayman Chemical
Sourced in United States

The ATP detection kit is a laboratory tool designed to measure the levels of adenosine triphosphate (ATP) in a sample. ATP is a critical molecule involved in cellular energy metabolism and is present in all living organisms. The kit provides a method for the quantitative analysis of ATP, enabling researchers to study various biological processes and cellular activities.

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2 protocols using atp detection kit

1

Measuring ATP Levels in BeWo Cells

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The ATP levels were measured in the BeWo cells exposed to buffer or MeHg-Cys (100, 250, 500 µM) for 30 min at 37 °C. The concentration of MeHg-Cys was increased in order to see detectable changes. The ATP levels were measured using an ATP detection kit from Cayman Chemical according to the manufacturer’s instructions.
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2

Platelet Signaling Pathway Analysis

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The supply of 5-hydroxyindolin-2-one (5-HI) was purchased from ChemFaces (Wuhan, China). Fura 2-AM (2-acetoxymethyl) and fibrinogen (Alexa Fluor 488 conjugated) were purchased from Invitrogen (Eugene, OR, USA). A serotonin detection kit was purchased from Labor Diagnostika Nord GmbH and Co. (Nordhorn, Germany). Physiological agonists (collagen, U46619, and thrombin) were obtained from Chrono-Log Co. (Havertown, PA, USA). Cell Signaling (Beverly, MA, USA) supplied anti-phospho-p38MAPK, anti-phospho-inositol-3-phosphate receptor type I (Ser1756), anti-phospho-ERK (1/2), anti-phospho-VASP (Ser157), anti-phospho-cPLA2 (Ser505), anti-phosphor-PI3K (Tyr458), anti-phospho-Akt (Ser473), anti-β-actin, and anti-rabbit secondary antibodies. The thromboxane B2, cAMP, and ATP detection kit were purchased from Cayman Chemical (Ann Arbor, MI, USA).
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