Atp detection kit

The ATP levels were measured in the BeWo cells exposed to buffer or MeHg-Cys (100, 250, 500 µM) for 30 min at 37 °C. The concentration of MeHg-Cys was increased in order to see detectable changes. The ATP levels were measured using an ATP detection kit from Cayman Chemical according to the manufacturer’s instructions.

The supply of 5-hydroxyindolin-2-one (5-HI) was purchased from ChemFaces (Wuhan, China). Fura 2-AM (2-acetoxymethyl) and fibrinogen (Alexa Fluor 488 conjugated) were purchased from Invitrogen (Eugene, OR, USA). A serotonin detection kit was purchased from Labor Diagnostika Nord GmbH and Co. (Nordhorn, Germany). Physiological agonists (collagen, U46619, and thrombin) were obtained from Chrono-Log Co. (Havertown, PA, USA). Cell Signaling (Beverly, MA, USA) supplied anti-phospho-p38^MAPK, anti-phospho-inositol-3-phosphate receptor type I (Ser¹⁷⁵⁶), anti-phospho-ERK (1/2), anti-phospho-VASP (Ser¹⁵⁷), anti-phospho-cPLA₂ (Ser⁵⁰⁵), anti-phosphor-PI3K (Tyr⁴⁵⁸), anti-phospho-Akt (Ser⁴⁷³), anti-β-actin, and anti-rabbit secondary antibodies. The thromboxane B₂, cAMP, and ATP detection kit were purchased from Cayman Chemical (Ann Arbor, MI, USA).