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Gpx cellular activity assay kit

Manufactured by Merck Group
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The Gpx Cellular activity assay Kit is a laboratory equipment designed to measure the activity of the enzyme Glutathione Peroxidase (Gpx) in cellular samples. It provides a quantitative assessment of Gpx levels, which is an important indicator of cellular antioxidant status and oxidative stress response.

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Close spelling variants of this product

Glutathione Peroxidase Cellular Activity Assay Kit (GPx, GPx Cellular Activity Assay Kit CGP-1 GPx) cellular activity kit GPx assay kit Activity Assay Kits

2 protocols using gpx cellular activity assay kit

1

Antioxidant Enzyme Activity Assays

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The activities of catalase and Gpx were measured by a Catalase- specific activity assay kit (Abcam) and a Gpx Cellular activity assay Kit (Sigma) according to the manufacturers’ protocols, respectively. MnSOD activities were measured by the nitroblue tetrazolium-bathocuproin sulfonate reduction inhibition method. Sodium cyanide (2 mM) was used to inhibit CuZnSOD activity as a previous study described (26 (link)).
Wei X., Xu Y., Xu F.F., Chaiswing L., Schnell D., Noel T., Wang C., Chen J., St Clair D.K, & St Clair W.H. (2017). RelB expression determines the differential effects of ascorbic acid in normal and cancer cells. Cancer research, 77(6), 1345-1356.
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2

Measuring Glutathione Peroxidase Activity

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Glutathione peroxidase works by reducing peroxides by oxidizing glutathione. The glutathione is then restored for further cycles of catalysis (Fig. 1). The rate-limiting step of this reaction is that in which the oxidized glutathione used to reduce the peroxide is restored via the oxidation of NADPH. NADPH absorbs at 340 nm. The selenium-dependent GPx activity was measured by using the Sigma-Aldrich GPx Cellular Activity Assay Kit (Sigma-Aldrich, St. Louis, MO, USA) following manufacturer instructions.

Reduced and oxidized states of glutathione

Qasem A., Abdel-Aty A., Abu-Suwa H, & Naser S.A. (2016). Oxidative stress due to Mycobacterium avium subspecies paratuberculosis (MAP) infection upregulates selenium-dependent GPx activity. Gut Pathogens, 8, 12.
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