Fixed, paraffin-embedded kidney sections (4 or 10 μm) were incubated with Celestine blue to stain for nuclei, and counterstained with haematoxylin (Amber Scientific, Australia) and either 0.3% Picrosirius red solution (Polysciences Inc., USA) or eosin (Amber Scientific, Australia). Sections were imaged using a bright-field (Leica Biosystems, Germany) or polarized microscope (Olympus, Japan) and analysed for percentage collagen content by ImageJ. Changes in renal tubular structure were assessed using a four-point scoring system as follows: 0 = no damage; 1 = mild damage (<25% tubules affected); 2 = moderate damage (25–50% of tubules affected); and 3 = severe damage (>50% of tubules affected). Quantification/scoring of Picrosirius red staining and renal histopathology parameters was performed by investigators who were blinded to the in vivo treatment corresponding to each sample.
Polarized microscope
Manufactured by Olympus
Sourced in Germany
A polarized microscope is an optical instrument that uses polarized light to analyze and observe samples. It is designed to study the optical properties of materials, particularly those that exhibit birefringence or double refraction. The core function of a polarized microscope is to provide a detailed view of the internal structure and composition of transparent or translucent samples, enabling users to gather valuable information about the material's characteristics.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using polarized microscope
1
Quantifying Renal Fibrosis and Tubular Damage
2
Masson Staining for Perivascular Fibrosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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After deparaffinization and rehydration, the 5 μm cross-sections were stained with Masson staining and photographed by a polarized microscope (Olympus, America) connected to a video camera (Nikon, Japan). Magnification was set up at ×200. Perivascular fibrosis was not assessed. The collagen volume fraction (CVF) was calculated using Image Pro Plus 6.0 as the percentage of red-stained area in the section.
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