Log In Sign up
The largest database of trusted experimental protocols

Anti rabbit igg pe

Manufactured by Jackson ImmunoResearch
Visit Supplier

Anti-Rabbit IgG-PE is a secondary antibody conjugated to the fluorescent dye Phycoerythrin (PE). It is used to detect and visualize rabbit primary antibodies in various immunoassays and cell-based applications.

Automatically generated - may contain errors

Lab products found in correlation

Mhcii, by Thermo Fisher Scientific (2 mentions) Fix perm buffer, by BD (2 mentions) Mouse anti inos, by Santa Cruz Biotechnology (2 mentions) Cd11b, by Thermo Fisher Scientific (2 mentions) F4 80, by Thermo Fisher Scientific (2 mentions) Lsrii flow cytometer, by BD (2 mentions) Facscalibur machine, by BD (1 mentions)

Spelling variants of this product

Rabbit anti-IgG (2 citations) Rabbit IgGs (2 citations)

5 protocols using anti rabbit igg pe

1

Measuring p-eIF2α in DSS-treated mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mice were orally gavaged with 2% DSS in 200μl volume. The LP cells were isolated and immediately fixed using the BD fixation solution for 10 minutes. Fixed cells were stained for intracellular p-eIF2α using a monoclonal antibody from Cell Signaling (3398). Cells were then stained with anti –Rabbit IgG-PE (Jackson labs, Cat No-111-116-144) at a 1:400 dilution.
Ravindran R., Loebbermann J., Nakaya H.I., Khan N., Ma H., Gama L., Machiah D.K., Lawson B., Hakimpour P., Wang Y.C., Li S., Sharma P., Kaufman R.J., Martinez J, & Pulendran B. (2016). The amino acid sensor GCN2 controls gut inflammation by inhibiting inflammasome activation. Nature, 531(7595), 523-527.
+ Open protocol
+ Expand
2

Medulloblastoma Single-Cell Immunophenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols
Medulloblastomas were dissected and dissociated. Cells were fixed and permeabilized in 4% PFA with 0.1% saponin (Sigma-Aldrich 47036) for 30 min at 4 °C. The primary antibodies used were against EED (Cell Signaling Technology Cat# 85322,) and CRE (Cell Signaling Technology Cat# 15036, RRID:AB_2798694). The secondary antibody used was anti-rabbit IgG-PE (#111-116-144, Jackson Immunoresearch Laboratory). Stained cells were analyzed with a BD FACSCalibur machine (BD Bioscience). The Flowjo software (RRID:SCR_008520) was used for data analyses.
Yi J., Kim B., Shi X., Zhan X., Lu Q.R., Xuan Z, & Wu J. (2022). PRC2 Heterogeneity Drives Tumor Growth in Medulloblastoma. Cancer research, 82(16), 2874-2886.
+ Open protocol
+ Expand
3

Measuring p-eIF2α in DSS-treated mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mice were orally gavaged with 2% DSS in 200μl volume. The LP cells were isolated and immediately fixed using the BD fixation solution for 10 minutes. Fixed cells were stained for intracellular p-eIF2α using a monoclonal antibody from Cell Signaling (3398). Cells were then stained with anti –Rabbit IgG-PE (Jackson labs, Cat No-111-116-144) at a 1:400 dilution.
Ravindran R., Loebbermann J., Nakaya H.I., Khan N., Ma H., Gama L., Machiah D.K., Lawson B., Hakimpour P., Wang Y.C., Li S., Sharma P., Kaufman R.J., Martinez J, & Pulendran B. (2016). The amino acid sensor GCN2 controls gut inflammation by inhibiting inflammasome activation. Nature, 531(7595), 523-527.
+ Open protocol
+ Expand
4

Peritoneal Immune Cell Profiling by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell subpopulations in the peritoneal lavage were identified by flow cytometry analysis via staining with fluorochrome-conjugated antibodies against the following antigens: Ly-6G, MHC II, CD11c, F4/80, CD11b, CD86 (eBioscience). For iNOS expression analysis, cells were fixed and permeabilized with Fix/Perm Buffer (BD Biosciences) and stained with mouse anti-iNOS (Santa Cruz Biotechnology) and PE-anti-rabbit IgG (Jackson ImmunoResearch Lab). Samples were acquired using a LSRII flow cytometer (BD) and analyzed with FlowJo software (TreeStar).
Vitturi D.A., Minarrieta L., Salvatore S.R., Postlethwait E.M., Fazzari M., Ferrer-Sueta G., Lancaster JR J.r., Freeman B.A, & Schopfer F.J. (2015). Convergence of Biological Nitration and Nitrosation via Symmetrical Nitrous Anhydride. Nature chemical biology, 11(7), 504-510.
+ Open protocol
+ Expand
5

Peritoneal Immune Cell Profiling by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell subpopulations in the peritoneal lavage were identified by flow cytometry analysis via staining with fluorochrome-conjugated antibodies against the following antigens: Ly-6G, MHC II, CD11c, F4/80, CD11b, CD86 (eBioscience). For iNOS expression analysis, cells were fixed and permeabilized with Fix/Perm Buffer (BD Biosciences) and stained with mouse anti-iNOS (Santa Cruz Biotechnology) and PE-anti-rabbit IgG (Jackson ImmunoResearch Lab). Samples were acquired using a LSRII flow cytometer (BD) and analyzed with FlowJo software (TreeStar).
Vitturi D.A., Minarrieta L., Salvatore S.R., Postlethwait E.M., Fazzari M., Ferrer-Sueta G., Lancaster JR J.r., Freeman B.A, & Schopfer F.J. (2015). Convergence of Biological Nitration and Nitrosation via Symmetrical Nitrous Anhydride. Nature chemical biology, 11(7), 504-510.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!