Apc cy7 anti hla dr l243

DCs were stained with the following antibody panel: phycoerythrin (PE)- anti-CD80 (2D10), PerCP-Cy5.5- anti-CD86 (IT2.2), Pe-Cy7- anti-CD83 (HB15), Brilliant Violet 421- anti-PD-L1 (29E.2A3), Brilliant Violet 510- anti-CD11c (3.9), allophycocyanin (APC)- anti-CD54 (HA58) and APC-Cy7- anti-HLA-DR (L243) (Biolegend, San Diego, CA, USA). Samples were acquired on DAKO CyAn ADP flow cytometer (Beckman Coulter, Brea, CA, USA) with compensation performed with positive and negative compensation beads (BD Biosciences, San Jose, CA, USA). Gating on and analysis of CD11c⁺ cells was performed using FlowJo software (Tree Star Inc., Ashland, OR, USA).

DCs were stained with the following antibody panel: phycoerythrin (PE)- anti-CD80 (2D10), PerCP-Cy5.5- anti-CD86 (IT2.2), Pe-Cy7- anti-CD83 (HB15), Brilliant Violet 421- anti-PD-L1 (29E.2A3), Brilliant Violet 510- anti-CD11c (3.9), allophycocyanin (APC)- anti-CD54 (HA58), and APC-Cy7- anti-HLA-DR (L243) (Biolegend). DC preparations were acquired on DAKO CyAn ADP flow cytometer (Beckman Coulter) with compensation performed with positive and negative antibody capture beads (BD Biosciences). Gating on and analysis of CD11c + cells was performed using FlowJo software (Tree Star Inc.) to determine Mean Fluorescence Intensity (MFI). The gating strategy and Fluorescence Minus One (FMO) staining controls are shown (Supplementary Fig. 1 B-C) and representative histograms are graphed (Supplementary Fig. 2).